Difference Between Western, Northern, and Southern Blot

Name: Difference Between Western, Northern, and Southern Blot
Creator: AAT Bioquest
License: https://www.aatbio.com/tou.html

Technique ▲ ▼	Western Blot ▲ ▼	Northern Blot ▲ ▼	Southern Blot ▲ ▼
Target Molecule	Protein	RNA	DNA
Sample Prep	Protein extraction	RNA isolation	DNA extraction
Separation	Gel Electrophoresis/SDS-PAGE	Gel Electrophoresis	Gel Electrophoresis
Membrane Material	Nitrocellulose/PVDF	Nylon	Nylon
Probe	Uses a single primary antibody or a combination of primary and secondary antibodies conjugated to either a fluorophore or enzyme	RNA, DNA or oligodeoxynucleotide	Nucleic acid probe
Detection	Film, CCD camera, LED or infrared imaging system	X-Ray film, chemiluminescence	X-Ray film, chemiluminescence

References

MLA	"Quest Database™ Difference Between Western, Northern, and Southern Blot." AAT Bioquest, Inc., 21 Aug. 2025, https://www.aatbio.com/data-sets/difference-between-western-northern-and-southern-blot.
APA	AAT Bioquest, Inc. (2025, August 21). Quest Database™ Difference Between Western, Northern, and Southern Blot. AAT Bioquest. https://www.aatbio.com/data-sets/difference-between-western-northern-and-southern-blot.
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