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Fluorochrome Brightness Reference for Flow Cytometry
Brightness scale is 1 (dimmest) to 10 (brightest). Brightness is computed as a function of extinction coefficient and quantum yield.
A PDF version of this chart is available for download here.
| Fluorophore | Spectrum Viewer | Ex | Em | Filter(nm) | Laser Line | Brightness |
|---|---|---|---|---|---|---|
| Alexa Fluor 350 | Link | 346 | 442 | 450/40 | 355 | 2 |
| iFluor 350 | Link | 345 | 450 | 450/40 | 355 | 6 |
| mFluor UV375 | Link | 351 | 387 | 450/40 | 355 | 2 |
| mFluor UV455 | Link | 357 | 461 | 450/40 | 355 | 2 |
| mFluor UV460 | Link | 358 | 456 | 450/40 | 355 | 2 |
References
This online resource may be cited as follows
MLA | "Quest Database™ Fluorochrome Brightness Reference for Flow Cytometry." AAT Bioquest, Inc., 8 Oct. 2025, https://www.aatbio.com/data-sets/fluorochrome-brightness-reference-for-flow-cytometry. | |
APA | AAT Bioquest, Inc. (2025, October 8). Quest Database™ Fluorochrome Brightness Reference for Flow Cytometry. AAT Bioquest. https://www.aatbio.com/data-sets/fluorochrome-brightness-reference-for-flow-cytometry. | |
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