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AAT Bioquest

Overview of Apoptosis Assays and Key Features

Parameters Measured
Probes
Key Features
Plasma Membrane Alterations (PS Exposure)Annexin Binding Assay
  • Detect early apoptosis markers
  • Flow cytometry or immunofluorescence application
Caspase Activation (Cytoplasm)Caspase Activity Assay
  • Quick, easy and high throughput
Caspase Binding (Cytoplasm)Fluorescent Caspase Inhibitors
  • ELISA, Flow Ctometry, or Western Blot
DNA Fragmentation (Nucleus)BrdU Assay and TUNEL Assay
  • Work with adherent cells, conjugated single cell resolution with cell cycle analysis by flow cytometry
Mitochondrial ChangesMitochondrial Stains
  • Fast, easy, single cell resolution using flow cytometry, fluorescence microscopy or fluorescence microplate reader



References

This online tool may be cited as follows

MLA

"Quest Database™ Overview of Apoptosis Assays and Key Features." AAT Bioquest, Inc.17 Jul2025https://www.aatbio.com/data-sets/overview-of-apoptosis-assays-and-key-features.

APA

AAT Bioquest, Inc. (2025July 17). Quest Database™ Overview of Apoptosis Assays and Key Features. AAT Bioquest. https://www.aatbio.com/data-sets/overview-of-apoptosis-assays-and-key-features.
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