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Paraffin-Embedded Tissue vs. Frozen Tissue
Paraffin-embedded tissue
Frozen tissue
FixationThe tissue sample is fixed in formaldehyde before being embedded in paraffin wax.The tissue sample is snap-frozen by immersing in liquid nitrogen or isopentane and then fixed in alcohol.
Sectioning InstrumentMicrotomeCryostat
StorageStable for multiple years at room temperatureStore at -80 °C for 12 months
Advantages
  • Preserves tissue morphology
  • Cost-effective, ambient storage conditions
  • Best suited for tissue-archiving
  • Quicker protocol does not require de-paraffinization or antigen retrieval steps
  • Preserves enzyme and antigen function
  • Does not require an initial fixation step like FFPE tissues
Limitations
  • The procedure is laborious and time-consuming
  • Proteins become denatured
  • Poor morphology
  • Decreased resolution at high magnification
  • Special storage requirements

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References
This online resource may be cited as follows
MLA
"Quest Database™ Paraffin-Embedded Tissue vs. Frozen Tissue." AAT Bioquest, Inc., 28 May. 2026, https://www.aatbio.com/data-sets/paraffin-embedded-tissue-vs-frozen-tissue.
APA
AAT Bioquest, Inc. (2026, May 28). Quest Database™ Paraffin-Embedded Tissue vs. Frozen Tissue. AAT Bioquest. https://www.aatbio.com/data-sets/paraffin-embedded-tissue-vs-frozen-tissue.
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