Properties and main applications of common detergents.

Name: Properties and main applications of common detergents.
Creator: AAT Bioquest
License: https://www.aatbio.com/tou.html

Detergent ▲ ▼	Properties ▲ ▼	Type ▲ ▼	Aggregation No. ▲ ▼	MW mono (micelle) ▲ ▼	CMC mM (%w/v) ▲ ▼	Cloud point °C ▲ ▼	Dialyzable ▲ ▼	Application ▲ ▼
SDS (sodium dodecyl sulfate)	Denaturing, strong lysis agent	Anionic	62	18,000	6-8 (0.17-0.23)	>100	No	Cell lysis, Electrophoresis, WB, Hybridization
Triton X-100	Non-denaturing, mild lysis agent	Non-ionic	140	90,000	0.24 (0.0155)	64	No	Cell culture, Enzyme immunoassays, IP, Membrane protein solubilization
Triton X-114	Non-denaturing, mild lysis agent	Non-ionic	-	-	0.21 (0.0113)	23	No	Cell lysis, Various routine protein and molecular biology techniques
NP-40	Non-denaturing, mild lysis agent	Non-ionic	149	90,000	0.29 (0.0179)	80	No	Isoelectric focusing
n-dodecyl-Β-D-maltoside	-	Non-ionic	98	50,000	0.17 (0.009)	-	No	Protein Crystallization
Brij 35	-	Non-ionic	40	49,000	0.09 (0.011)	>100	No	Cell lysis, Chromatography, Electrophoresis, HPLC, Membrane protein solubilization
Digitonin	Non-denaturing, mild lysis agent	Non-ionic	60	70,000	-	-	No	Membrane solubilization, Protein solubilization, Enzymology, Chromatography, Cell culture
Tween-20	Mild lysis agent	Non-ionic	-	-	0.06 (0.0074)	95	No	Cell lysis (mammalian cells), WB, ELISA, Enzyme immunoassays, Membrane protein solubilization
Tween-80	Mild lysis agent	Non-ionic	60	76,000	0.01 (0.0016)	-	No	ELISA, WB, Immunoassays
Octyl-beta-Glucoside	Non-denaturing, mild lysis agent	Non-ionic	27	8,000	23-24 (∼0.70)	>100	Yes	Membrane protein solubilization
Octylthio Glucoside	Non-denaturing, mild lysis agent	Non-ionic	-		9 (0.2772)	>100	Yes	Cell lysis, Protein solubilization
CHAPS	Mild lysis agent	Zwitterionic	10	6,000	8-10 (0.5-0.6)	>100	Yes	Cell lysis, 2D electrophoresis, isoelectric focusing, IP, glycoprotein electrophoresis, membrane protein solubilization, DNA extraction
CHAPSO	Mild lysis agent	Zwitterionic	11	7,000	8-10 (∼0.5-0.6)	90	Yes	Solubilization of integral membrane proteins.

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APA	AAT Bioquest, Inc. (2025, September 1). Quest Database™ Properties and main applications of common detergents.. AAT Bioquest. https://www.aatbio.com/data-sets/properties-and-main-applications-of-common-detergents.
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Properties and main applications of common detergents.

References

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