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Properties and main applications of common detergents.

Detergent
Properties
Type
Aggregation No.
MW mono (micelle)
CMC mM (%w/v)
Cloud point °C
Dialyzable
Application
SDS (sodium dodecyl sulfate)Denaturing, strong lysis agentAnionic6218,0006-8 (0.17-0.23)>100NoCell lysis, Electrophoresis, WB, Hybridization
Triton X-100Non-denaturing, mild lysis agentNon-ionic14090,0000.24 (0.0155)64NoCell culture, Enzyme immunoassays, IP, Membrane protein solubilization
Triton X-114Non-denaturing, mild lysis agentNon-ionic--0.21 (0.0113)23NoCell lysis, Various routine protein and molecular biology techniques
NP-40Non-denaturing, mild lysis agentNon-ionic14990,0000.29 (0.0179)80NoIsoelectric focusing
n-dodecyl-Β-D-maltoside-Non-ionic9850,0000.17 (0.009)-NoProtein Crystallization
Brij 35-Non-ionic4049,0000.09 (0.011)>100NoCell lysis, Chromatography, Electrophoresis, HPLC, Membrane protein solubilization
DigitoninNon-denaturing, mild lysis agentNon-ionic6070,000--NoMembrane solubilization, Protein solubilization, Enzymology, Chromatography, Cell culture
Tween-20Mild lysis agentNon-ionic--0.06 (0.0074)95NoCell lysis (mammalian cells), WB, ELISA, Enzyme immunoassays, Membrane protein solubilization
Tween-80Mild lysis agentNon-ionic6076,0000.01 (0.0016)-NoELISA, WB, Immunoassays
Octyl-beta-GlucosideNon-denaturing, mild lysis agentNon-ionic278,00023-24 (∼0.70)>100YesMembrane protein solubilization
Octylthio GlucosideNon-denaturing, mild lysis agentNon-ionic-9 (0.2772)>100YesCell lysis, Protein solubilization
CHAPSMild lysis agentZwitterionic106,0008-10 (0.5-0.6)>100YesCell lysis, 2D electrophoresis, isoelectric focusing, IP, glycoprotein electrophoresis, membrane protein solubilization, DNA extraction
CHAPSOMild lysis agentZwitterionic117,0008-10 (∼0.5-0.6)90YesSolubilization of integral membrane proteins.



References

This online tool may be cited as follows

MLA

"Quest Database™ Properties and main applications of common detergents.." AAT Bioquest, Inc.16 Jun2024https://www.aatbio.com/data-sets/properties-and-main-applications-of-common-detergents.

APA

AAT Bioquest, Inc. (2024June 16). Quest Database™ Properties and main applications of common detergents.. AAT Bioquest. https://www.aatbio.com/data-sets/properties-and-main-applications-of-common-detergents.
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