Name: iFluor® 647 succinimidyl ester
Brand: AAT Bioquest
SKU: 1031
Availability: InStock

iFluor® 647 succinimidyl ester

iFluor® 647 succinimidyl ester is an amine-reactive, far-red fluorescent dye designed for covalent labeling of proteins, particularly antibodies, for fluorescence detection applications.

Amine-Reactive Labeling: Exhibits good reactivity and selectivity toward protein amino groups.
Spectral Properties: Excitation/emission maxima of 655/670 nm.
pH Stability: Exhibits pH-independent fluorescence from pH 3 to 11.
Photostability: Provides stronger fluorescence and higher photostability than Cy5®.

<p>HeLa cells were stained with mouse anti-tubulin followed with iFluor<sup>TM</sup> 647 goat anti-mouse IgG (H+L) (red); and nuclei were stained with DAPI (blue).</p>

Protocol

Catalog	Size	Price
1031	1 mg	Price
71031	100 ug	Price
71510	5 mg	Price
71560	10 mg	Price

Overview

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). The iFluor® 647 family has spectral properties essentially identical to those of Cy5®. Compared to Cy5® probes, iFluor® 647 reagents have much stronger fluorescence and higher photostability. Their fluorescence is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family an excellent alternative to Cy5® and Alexa Fluor® 647 (Cy5® and Alexa Fluor® are the trademarks of GE Health Care and Invitrogen). iFluor® 647 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 647 succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	78.452 µL	392.261 µL	784.523 µL	3.923 mL	7.845 mL
5 mM	15.69 µL	78.452 µL	156.905 µL	784.523 µL	1.569 mL
10 mM	7.845 µL	39.226 µL	78.452 µL	392.261 µL	784.523 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1274.66 g/mol	=	mL	x	mM	=	mmol

Citations

View all 80 citations: Citation Explorer

Structural and mechanistic insights into the fungal glycosylphosphatidylinositol mannosyltransferase I complex

Authors:

Liu, Jia and Yang, Yizheng and Tan, Yi and Hua, Zhengkang and Hu, Xinlin and Ding, Xuyang and Yang, Ping and Ke, Yan and Zhang, Zhentao and Li, Tianlu and others

Journal:

Proceedings of the National Academy of Sciences (2026): e2617140123

Architecture and mechanism of the human p24 cargo receptor

Authors:

Hua, Zhengkang and Zhang, Di and Zhang, Min and Hu, Xinlin and Kang, Chengtao and Sun, Huanhuan and Yang, Ping and Qi, Chu and Lin, Xinyuan and Li, Jiameng and others

Journal:

Proceedings of the National Academy of Sciences (2026): e2610235123

Atomic Force Microscopy Based Single Molecule Recognition: Deciphering Biological Interactions at the Nanoscale

Authors:

Zhang, Huaiwei and Xu, Qianhui and Chen, Junmei and Chen, Danhong and Zhong, Haijian and Zhao, Weidong

Journal:

Journal of Molecular Recognition (2026): e70042

Immune modulatory oncolytic virus for osteosarcoma therapy

Authors:

Khan, Sumbul and Higgins, Theresa A and Shimko-Lofano, Isabella and Sandey, Maninder and Logsdon, Emma and Bunch, Gracie and Fatemi, Yasin and Hoffman, Leah MK and Smith, Bruce F and Agarwal, Payal

Systematic investigation of the effects of neural stem cell spheroid size and density on fate specification in 3D culture

Authors:

Duquette, Rebecca and Salazar, Sabrina Pietrosemoli and Wang, Ze Zhong and Sohrabi, Alireza and Seidlits, Stephanie K

Journal:

Journal of Materials Chemistry B (2026)

References

View all 49 references: Citation Explorer

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes

Authors:

Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.

Journal:

Mol Biosyst (2011): 1766

Visualizing dengue virus through Alexa Fluor labeling

Authors:

Zhang S, Tan HC, Ooi EE.

Journal:

J Vis Exp. (2011)

Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells

Authors:

Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.

Journal:

Biochem Biophys Res Commun (2011): 211

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594

Authors:

Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.

Journal:

Zhen Ci Yan Jiu (2011): 262

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers

Authors:

Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.

Journal:

J Microbiol Methods (2011): 119

Physical properties

Molecular weight	1274.66
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.03
Correction factor (280 nm)	0.03
Correction factor (656 nm)	0.0793
Extinction coefficient (cm ^-1 M ^-1)	220000 ¹
Excitation (nm)	655
Emission (nm)	670
Quantum yield	0.25 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on November 8, 2026