10XHis azide
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1641.71 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
10XHis Succinimidyl Ester |
10XHis maleimide |
10XHis alkyne |
Overview | SDSProtocol |
See also: Click Chemistry
Molecular weight 1641.71 |
10XHis azide is an excellent building block to make 10XHis conjugates for developing His tag detection probes and purification tools. It readily reacts with a biomolecule that contains an alkyne group, such as alkyne-modified antibodies, peptides and oligos. We found that it can be readily conjugated to a DBCO-modified molecule in extremely high yield. The 10XHis tag is one of the most common His tags and has a molecular weight of ~2kDa. His-tags (i.e., polyhistidine tags) comprise a consecutive series of six to ten histidine residues. His tags are used for many recombinant proteins to facilitate purification, allowing researchers to extract a protein of interest from thousands of other proteins found in a cell or cell lysate. The small size of the 10X-His tag has a lower possibility for the tag to affect the functionality of the fusion protein. Histidine forms coordination bonds with immobilized transition metal ions, and this property can be utilized for protein purification. His-tag protein purification is by a specialized form of affinity chromatography, called immobilized metal affinity chromatography (IMAC), where proteins or peptides are separated according to their affinity for metal ions immobilized to a solid chelating resin. During this process, a small His-tag is fused to either the N or C terminus of the target protein, enabling capture by nickel or cobalt ions coordinated on a variety of resins. The small size, low cost, and ease of use have made the His-tag the most popular affinity-tag available. AAT Bioquest offers the largest collection of 6XHis, 0XHis, NTA, bis-NTA, tris-NTA and IDA reagents for His Tag detections and purifications.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 10XHis azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 60.912 µL | 304.56 µL | 609.121 µL | 3.046 mL | 6.091 mL |
5 mM | 12.182 µL | 60.912 µL | 121.824 µL | 609.121 µL | 1.218 mL |
10 mM | 6.091 µL | 30.456 µL | 60.912 µL | 304.56 µL | 609.121 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
AMCA Azide | 346 | 434 | 19000 | 0.153 |
XFD488 azide *Same Structure to Alexa Fluor™ 488 azide* | 499 | 520 | 71000 | 0.11 |
ICG azide | 789 | 813 | 230000 | 0.076 |
Cy3B azide | 560 | 571 | 1200001 | 0.069 |
XFD647 Azide | 650 | 671 | 270000 | 0.03 |
Images
References
View all 2 references: Citation Explorer
Selective Derivatization of Hexahistidine-Tagged Recombinant Proteins.
Authors: Kadambar, Vasantha Krishna and Melman, Artem
Journal: Advances in experimental medicine and biology (2019): 237-250
Authors: Kadambar, Vasantha Krishna and Melman, Artem
Journal: Advances in experimental medicine and biology (2019): 237-250
Site-specific DOTA/europium-labeling of recombinant human relaxin-3 for receptor-ligand interaction studies.
Authors: Zhang, Wei-Jie and Luo, Xiao and Liu, Ya-Li and Shao, Xiao-Xia and Wade, John D and Bathgate, Ross A D and Guo, Zhan-Yun
Journal: Amino acids (2012): 983-92
Authors: Zhang, Wei-Jie and Luo, Xiao and Liu, Ya-Li and Shao, Xiao-Xia and Wade, John D and Bathgate, Ross A D and Guo, Zhan-Yun
Journal: Amino acids (2012): 983-92