10XHis maleimide
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1709.78 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
10XHis Succinimidyl Ester |
10XHis azide |
10XHis alkyne |
Overview | SDSProtocol |
Molecular weight 1709.78 |
10XHis maleimide is an excellent building block to make 10XHis conjugates for developing His tag detection probes and purification tools. It readily reacts with a biomolecule that contains a thiol group such as antibodies, peptides and thiol-modified oligos. The 10XHis tag is one of the most common His tags and has a molecular weight of ~2kDa. His-tags (i.e., polyhistidine tags) comprise a consecutive series of six to ten histidine residues. His tags are used for many recombinant proteins to facilitate purification, allowing researchers to extract a protein of interest from thousands of other proteins found in a cell or cell lysate. The small size of the 10X-His tag has a lower possibility for the tag to affect the functionality of the fusion protein. Histidine forms coordination bonds with immobilized transition metal ions, and this property can be utilized for protein purification. His-tag protein purification is by a specialized form of affinity chromatography, called immobilized metal affinity chromatography (IMAC), where proteins or peptides are separated according to their affinity for metal ions immobilized to a solid chelating resin. During this process, a small His-tag is fused to either the N or C terminus of the target protein, enabling capture by nickel or cobalt ions coordinated on a variety of resins. The small size, low cost, and ease of use have made the His-tag the most popular affinity-tag available. AAT Bioquest offers the largest collection of 6XHis, 0XHis, NTA, bis-NTA, tris-NTA and IDA reagents for His Tag detections and purifications.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 10XHis maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 58.487 µL | 292.435 µL | 584.871 µL | 2.924 mL | 5.849 mL |
5 mM | 11.697 µL | 58.487 µL | 116.974 µL | 584.871 µL | 1.17 mL |
10 mM | 5.849 µL | 29.244 µL | 58.487 µL | 292.435 µL | 584.871 µL |
Molarity calculator
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Images
References
View all 44 references: Citation Explorer
Selective APC-targeting of a novel Fc-fusion multi-immunodominant recombinant protein (tTax-tEnv:mFcγ2a) for HTLV-1 vaccine development.
Authors: Shafifar, Mina and Mozhgani, Sayed-Hamidreza and Razavi Pashabayg, Kobra and Mosavat, Arman and Karbalaei, Mohsen and Norouzi, Mehdi and Rezaee, Seyed Abdolrahim
Journal: Life sciences (2022): 120920
Authors: Shafifar, Mina and Mozhgani, Sayed-Hamidreza and Razavi Pashabayg, Kobra and Mosavat, Arman and Karbalaei, Mohsen and Norouzi, Mehdi and Rezaee, Seyed Abdolrahim
Journal: Life sciences (2022): 120920
Reshaping nanobodies for affinity purification on protein a.
Authors: Crauwels, Maxine and Van Vaerenbergh, Nele and Kulaya, Neeme Benedict and Vincke, Cécile and D'Huyvetter, Matthias and Devoogdt, Nick and Muyldermans, Serge and Xavier, Catarina
Journal: New biotechnology (2020): 20-28
Authors: Crauwels, Maxine and Van Vaerenbergh, Nele and Kulaya, Neeme Benedict and Vincke, Cécile and D'Huyvetter, Matthias and Devoogdt, Nick and Muyldermans, Serge and Xavier, Catarina
Journal: New biotechnology (2020): 20-28
Simplified detection of polyhistidine-tagged proteins in gels and membranes using a UV-excitable dye and a multiple chelator head pair.
Authors: Raducanu, Vlad-Stefan and Isaioglou, Ioannis and Raducanu, Daniela-Violeta and Merzaban, Jasmeen S and Hamdan, Samir M
Journal: The Journal of biological chemistry (2020): 12214-12223
Authors: Raducanu, Vlad-Stefan and Isaioglou, Ioannis and Raducanu, Daniela-Violeta and Merzaban, Jasmeen S and Hamdan, Samir M
Journal: The Journal of biological chemistry (2020): 12214-12223
A bispecific circular aptamer tethering a built-in universal molecular tag for functional protein delivery.
Authors: Pan, Xiaoshu and Yang, Yu and Li, Long and Li, Xiaowei and Li, Qiang and Cui, Cheng and Wang, Bang and Kuai, Hailan and Jiang, Jianhui and Tan, Weihong
Journal: Chemical science (2020): 9648-9654
Authors: Pan, Xiaoshu and Yang, Yu and Li, Long and Li, Xiaowei and Li, Qiang and Cui, Cheng and Wang, Bang and Kuai, Hailan and Jiang, Jianhui and Tan, Weihong
Journal: Chemical science (2020): 9648-9654
The surface syndecan protein from Macrobrachium rosenbergii could function as mediator in bacterial infections.
Authors: Yang, Hui and Xiong, Haoran and Mi, Kaihang and Zhang, Yingying and Zhang, Xiaojun and Chen, Guohong
Journal: Fish & shellfish immunology (2020): 62-68
Authors: Yang, Hui and Xiong, Haoran and Mi, Kaihang and Zhang, Yingying and Zhang, Xiaojun and Chen, Guohong
Journal: Fish & shellfish immunology (2020): 62-68
[Cloning and expression of SmDXS2 gene in Swertia mussotii].
Authors: Li, Wen-Jing and Xiang, Bei-Bei and Sun, Yan-Xiang and Hou, Xiao-Qiang and Han, Mei-Ling and Li, Xiao-Xue and Wang, Yong and Guo, Shuo
Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (2019): 935-941
Authors: Li, Wen-Jing and Xiang, Bei-Bei and Sun, Yan-Xiang and Hou, Xiao-Qiang and Han, Mei-Ling and Li, Xiao-Xue and Wang, Yong and Guo, Shuo
Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (2019): 935-941
Overexpression, Purification and Functional Characterisation of Wild-Type HIV-1 Subtype C Protease and Two Variants Using a Thioredoxin and His-Tag Protein Fusion System.
Authors: Zondagh, Jake and Williams, Alison and Achilonu, Ikechukwu and Dirr, Heini W and Sayed, Yasien
Journal: The protein journal (2018): 369-379
Authors: Zondagh, Jake and Williams, Alison and Achilonu, Ikechukwu and Dirr, Heini W and Sayed, Yasien
Journal: The protein journal (2018): 369-379
Molecular characterization of Babesia microti thioredoxin (BmTrx2) and its expression patterns induced by antiprotozoal drugs.
Authors: Huang, Jingwei and Xiong, Kang and Zhang, Houshuang and Zhao, Yanzhen and Cao, Jie and Gong, Haiyan and Zhou, Yongzhi and Zhou, Jinlin
Journal: Parasites & vectors (2018): 38
Authors: Huang, Jingwei and Xiong, Kang and Zhang, Houshuang and Zhao, Yanzhen and Cao, Jie and Gong, Haiyan and Zhou, Yongzhi and Zhou, Jinlin
Journal: Parasites & vectors (2018): 38
Characterization of Recombinant His-Tag Protein Immobilized onto Functionalized Gold Nanoparticles.
Authors: Torres-González, Lisa and Díaz-Ayala, Ramonita and Vega-Olivencia, Carmen A and López-Garriga, Juan
Journal: Sensors (Basel, Switzerland) (2018)
Authors: Torres-González, Lisa and Díaz-Ayala, Ramonita and Vega-Olivencia, Carmen A and López-Garriga, Juan
Journal: Sensors (Basel, Switzerland) (2018)
Facile fabrication of nickel immobilized on magnetic nanoparticles as an efficient affinity adsorbent for purification of his-tagged protein.
Authors: Rashid, Zahra and Naeimi, Hossein and Zarnani, Amir-Hassan and Mohammadi, Fereshteh and Ghahremanzadeh, Ramin
Journal: Materials science & engineering. C, Materials for biological applications (2017): 670-676
Authors: Rashid, Zahra and Naeimi, Hossein and Zarnani, Amir-Hassan and Mohammadi, Fereshteh and Ghahremanzadeh, Ramin
Journal: Materials science & engineering. C, Materials for biological applications (2017): 670-676