10XHis Succinimidyl Ester
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 2044.12 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
10XHis maleimide |
10XHis azide |
10XHis alkyne |
Overview | SDSProtocol |
Molecular weight 2044.12 |
10XHis succinimidyl ester is an excellent building block to make 10XHis conjugates for developing His tag detection probes and purification tools. It readily reacts with a biomolecule that contains an amino group such as antibodies, peptides and amino-modified oligos. The 10XHis tag is one of the most common His tags and has a molecular weight of ~2kDa. His-tags (i.e., polyhistidine tags) comprise a consecutive series of six to ten histidine residues. His tags are used for many recombinant proteins to facilitate purification, allowing researchers to extract a protein of interest from thousands of other proteins found in a cell or cell lysate. The small size of the 10X-His tag has a lower possibility for the tag to affect the functionality of the fusion protein. Histidine forms coordination bonds with immobilized transition metal ions, and this property can be utilized for protein purification. His-tag protein purification is by a specialized form of affinity chromatography, called immobilized metal affinity chromatography (IMAC), where proteins or peptides are separated according to their affinity for metal ions immobilized to a solid chelating resin. During this process, a small His-tag is fused to either the N or C terminus of the target protein, enabling capture by nickel or cobalt ions coordinated on a variety of resins. The small size, low cost, and ease of use have made the His-tag the most popular affinity-tag available. AAT Bioquest offers the largest collection of 6XHis, 0XHis, NTA, bis-NTA, tris-NTA and IDA reagents for His Tag detections and purifications.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 10XHis Succinimidyl Ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 48.921 µL | 244.604 µL | 489.208 µL | 2.446 mL | 4.892 mL |
5 mM | 9.784 µL | 48.921 µL | 97.842 µL | 489.208 µL | 978.416 µL |
10 mM | 4.892 µL | 24.46 µL | 48.921 µL | 244.604 µL | 489.208 µL |
Molarity calculator
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References
View all 50 references: Citation Explorer
Structural and biochemical insights into His-tag-induced higher-order oligomerization of membrane proteins by cryo-EM and size exclusion chromatography.
Authors: Ayoub, Nooraldeen and Roth, Patrick and Ucurum, Zöhre and Fotiadis, Dimitrios and Hirschi, Stephan
Journal: Journal of structural biology (2023): 107924
Authors: Ayoub, Nooraldeen and Roth, Patrick and Ucurum, Zöhre and Fotiadis, Dimitrios and Hirschi, Stephan
Journal: Journal of structural biology (2023): 107924
Ni aptamer: DNA mimic of His-tag to recognize Ni-NTA.
Authors: Jahan, Raunak and Silwal, Achut Prasad and Thennakoon, Siddhartha Kalpa Samadhi and Arya, Satya Prakash and Postema, Rick Mason and Timilsina, Hari and Reynolds, Andrew Michael and Tan, Xiaohong
Journal: Chemical communications (Cambridge, England) (2023): 12851-12854
Authors: Jahan, Raunak and Silwal, Achut Prasad and Thennakoon, Siddhartha Kalpa Samadhi and Arya, Satya Prakash and Postema, Rick Mason and Timilsina, Hari and Reynolds, Andrew Michael and Tan, Xiaohong
Journal: Chemical communications (Cambridge, England) (2023): 12851-12854
N-terminal LysSN-His-tag improves the production of intracellular recombinant protein in Bacillus subtilis.
Authors: Le, Ngan Thi Phuong and Phan, Trang Thi Phuong and Truong, Tuom Thi Tinh and Schumann, Wolfgang and Nguyen, Hoang Duc
Journal: Cell biochemistry and function (2023): 823-832
Authors: Le, Ngan Thi Phuong and Phan, Trang Thi Phuong and Truong, Tuom Thi Tinh and Schumann, Wolfgang and Nguyen, Hoang Duc
Journal: Cell biochemistry and function (2023): 823-832
Expression of a Copper Activated Xylanase in Yeast: Location of the His-Tag in the Protein Significantly Affects the Enzymatic Properties.
Authors: Elgharbi, Fatma and Ben Hlima, Hajer and Ben Mabrouk, Sameh and Hmida-Sayari, Aïda
Journal: Molecular biotechnology (2023): 1109-1118
Authors: Elgharbi, Fatma and Ben Hlima, Hajer and Ben Mabrouk, Sameh and Hmida-Sayari, Aïda
Journal: Molecular biotechnology (2023): 1109-1118
Development of a Modular NTA:His Tag Viral Vaccine for Co-delivery of Antigen and Adjuvant.
Authors: Chung, Young Hun and Volckaert, Britney A and Steinmetz, Nicole F
Journal: Bioconjugate chemistry (2023): 269-278
Authors: Chung, Young Hun and Volckaert, Britney A and Steinmetz, Nicole F
Journal: Bioconjugate chemistry (2023): 269-278
A novel His-tag-binding aptamer for recombinant protein detection and T cell-based immunotherapy.
Authors: Yang, Li-Ting and Abudureheman, Tuersunayi and Zheng, Wei-Wei and Zhou, Hang and Chen, Jing and Duan, Cai-Wen and Chen, Kai-Ming
Journal: Talanta (2023): 124722
Authors: Yang, Li-Ting and Abudureheman, Tuersunayi and Zheng, Wei-Wei and Zhou, Hang and Chen, Jing and Duan, Cai-Wen and Chen, Kai-Ming
Journal: Talanta (2023): 124722
The Highly Efficient Expression System of Recombinant Human Prolidase and the Effect of N-Terminal His-Tag on the Enzyme Activity.
Authors: Czyrko-Horczak, Justyna and Nizioł, Magdalena and Forlino, Antonella and Besio, Roberta and Miltyk, Wojciech
Journal: Cells (2022)
Authors: Czyrko-Horczak, Justyna and Nizioł, Magdalena and Forlino, Antonella and Besio, Roberta and Miltyk, Wojciech
Journal: Cells (2022)
Application of temperature-responsive HIS-tag fluorophores to differential scanning fluorimetry screening of small molecule libraries.
Authors: Ronzetti, Michael H and Baljinnyam, Bolormaa and Itkin, Zina and Jain, Sankalp and Rai, Ganesha and Zakharov, Alexey V and Pal, Utpal and Simeonov, Anton
Journal: Frontiers in pharmacology (2022): 1040039
Authors: Ronzetti, Michael H and Baljinnyam, Bolormaa and Itkin, Zina and Jain, Sankalp and Rai, Ganesha and Zakharov, Alexey V and Pal, Utpal and Simeonov, Anton
Journal: Frontiers in pharmacology (2022): 1040039
The His-tag as a decoy modulating preferred orientation in cryoEM.
Authors: Bromberg, Raquel and Cai, Kai and Guo, Yirui and Plymire, Daniel and Emde, Tabitha and Puzio, Maciej and Borek, Dominika and Otwinowski, Zbyszek
Journal: Frontiers in molecular biosciences (2022): 912072
Authors: Bromberg, Raquel and Cai, Kai and Guo, Yirui and Plymire, Daniel and Emde, Tabitha and Puzio, Maciej and Borek, Dominika and Otwinowski, Zbyszek
Journal: Frontiers in molecular biosciences (2022): 912072
Highly Selective Lysine Acylation in Proteins Using a Lys-His Tag Sequence.
Authors: Kofoed, Christian and Wu, Shunliang and Sørensen, Kasper K and Treiberg, Tuule and Arnsdorf, Johnny and Bjørn, Sara P and Jensen, Tanja L and Voldborg, Bjørn G and Thygesen, Mikkel B and Jensen, Knud J and Schoffelen, Sanne
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2022): e202200147
Authors: Kofoed, Christian and Wu, Shunliang and Sørensen, Kasper K and Treiberg, Tuule and Arnsdorf, Johnny and Bjørn, Sara P and Jensen, Tanja L and Voldborg, Bjørn G and Thygesen, Mikkel B and Jensen, Knud J and Schoffelen, Sanne
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2022): e202200147