BG Succinimidyl Ester

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Physical properties

Molecular weight	481.47
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Alternative formats

Overview SDS Protocol

Molecular weight

481.47

SNAP protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a protein based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). SNAP-tag substrates are fluorophores, biotin or beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag. BG Succinimidyl Ester is an amine-reactive building block for preparing of SNAP substrates from amine-containing labels or biological targets. BG Succinimidyl Ester building block facilities the development of various SNAP-tag substrates for labeling SNAP-tag fusion proteins for a wide range of biological applications.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of BG Succinimidyl Ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	207.697 µL	1.038 mL	2.077 mL	10.385 mL	20.77 mL
5 mM	41.539 µL	207.697 µL	415.395 µL	2.077 mL	4.154 mL
10 mM	20.77 µL	103.849 µL	207.697 µL	1.038 mL	2.077 mL

Molarity calculator

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Images

Figure 1. Chemical structure for BG Succinimidyl Ester.

References

View all 50 references: Citation Explorer

Photo-SNAP-tag, a Light-Regulated Chemical Labeling System.
Authors: Cleveland, Joseph D and Tucker, Chandra L
Journal: ACS chemical biology (2020)

The covalent SNAP tag for protein display quantification and low-pH protein engineering.
Authors: Jin, Wei and Madan, Bharat and Mussman, Brooklyn K and Hailemariam, Amen T and Fahad, Ahmed S and Wolfe, Jacy R and Kwon, Young Do and Zhang, Baoshan and Shapiro, Lawrence and Kwong, Peter D and DeKosky, Brandon J
Journal: Journal of biotechnology (2020): 50-56

Visualizing and Manipulating Biological Processes by Using HaloTag and SNAP-Tag Technologies.
Authors: Hoelzel, Conner A and Zhang, Xin
Journal: Chembiochem : a European journal of chemical biology (2020): 1935-1946

[Combined application of SNAP-tag and fluorescence technique in in-situ protein analysis].
Authors: Qiao, Qinglong and Zhou, Wei and Chen, Jie and Liu, Wenjuan and Miao, Lu and Yin, Wenting and Xu, Zhaochao
Journal: Se pu = Chinese journal of chromatography (2019): 872-877

A SNAP-tag fluorogenic probe mimicking the chromophore of the red fluorescent protein Kaede.
Authors: Jung, Kwan Ho and Fares, Matthew and Grainger, Leeann S and Wolstenholme, Charles H and Hou, Anna and Liu, Yu and Zhang, Xin
Journal: Organic & biomolecular chemistry (2019): 1906-1915

One-step site-specific antibody fragment auto-conjugation using SNAP-tag technology.
Authors: Hussain, Ahmad Fawzi and Heppenstall, Paul A and Kampmeier, Florian and Meinhold-Heerlein, Ivo and Barth, Stefan
Journal: Nature protocols (2019): 3101-3125

Applications of SNAP-tag technology in skin cancer therapy.
Authors: Padayachee, Eden Rebecca and Adeola, Henry Ademola and Van Wyk, Jennifer Catherine and Nsole Biteghe, Fleury Augustine and Chetty, Shivan and Khumalo, Nonhlanhla Patience and Barth, Stefan
Journal: Health science reports (2019): e103

Beyond O6-Benzylguanine: O6-(5-Pyridylmethyl)guanine as a Substrate for the Self-Labeling Enzyme SNAP-Tag.
Authors: Macias-Contreras, Miguel and He, Huan and Zhu, Lei
Journal: Bioconjugate chemistry (2018): 4104-4109

Imaging Newly Synthesized and Old Histone Variant Dynamics Dependent on Chaperones Using the SNAP-Tag System.
Authors: Torné, Júlia and Orsi, Guillermo A and Ray-Gallet, Dominique and Almouzni, Geneviève
Journal: Methods in molecular biology (Clifton, N.J.) (2018): 207-221

SNAP-Tag-Based Subcellular Protein Labeling and Fluorescent Imaging with Naphthalimides.
Authors: Wang, Chao and Song, Xinbo and Xiao, Yi
Journal: Chembiochem : a European journal of chemical biology (2017): 1762-1769