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BC Succinimidyl Ester

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Physical properties
Molecular weight441.44
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Alternative formats
BC-Maleimide

OverviewpdfSDSpdfProtocol


Molecular weight
441.44
CLIP-tag is a modified version of SNAP-tag. It is also a self-labeling protein derived from human O6-alkylguanine-DNA-alkyltransferase. Instead of benzylguanine derivatives, CLIP tag is engineered to react with benzylcytosine derivatives. It can be used as a protein tag for tagging your protein of interest (POI). BC Succinimidyl Ester is an amine-reactive building block for preparing of SNAP substrates from amine-containing labels or biological targets. BC Succinimidyl Ester building block facilities the development of various CLIP-tag substrates for labeling CLIP-tag fusion proteins for a wide range of biological applications.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of BC Succinimidyl Ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM226.531 µL1.133 mL2.265 mL11.327 mL22.653 mL
5 mM45.306 µL226.531 µL453.063 µL2.265 mL4.531 mL
10 mM22.653 µL113.266 µL226.531 µL1.133 mL2.265 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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References


View all 38 references: Citation Explorer
Modulation of emission and singlet oxygen photosensitisation in live cells utilising bioorthogonal phosphorogenic probes and protein tag technology.
Authors: Leung, Peter Kam-Keung and Lo, Kenneth Kam-Wing
Journal: Chemical communications (Cambridge, England) (2020): 6074-6077
Expanding the substrate selectivity of SNAP/CLIP-tagging of intracellular targets.
Authors: Macias-Contreras, Miguel and Little, Kevin N and Zhu, Lei
Journal: Methods in enzymology (2020): 233-257
Analysis of the nucleocytoplasmic shuttling RNA-binding protein HNRNPU using optimized HITS-CLIP method.
Authors: Yugami, Masato and Okano, Hideyuki and Nakanishi, Atsushi and Yano, Masato
Journal: PloS one (2020): e0231450
Visualization of Spirochetes by Labeling Membrane Proteins With Fluorescent Biarsenical Dyes.
Authors: Hillman, Chadwick and Stewart, Philip E and Strnad, Martin and Stone, Hunter and Starr, Tregei and Carmody, Aaron and Evans, Tyler J and Carracoi, Valentina and Wachter, Jenny and Rosa, Patricia A
Journal: Frontiers in cellular and infection microbiology (2019): 287
Quantifying transcription factor binding dynamics at the single-molecule level in live cells.
Authors: Presman, Diego M and Ball, David A and Paakinaho, Ville and Grimm, Jonathan B and Lavis, Luke D and Karpova, Tatiana S and Hager, Gordon L
Journal: Methods (San Diego, Calif.) (2017): 76-88
Rational Design and Applications of Semisynthetic Modular Biosensors: SNIFITs and LUCIDs.
Authors: Farrants, Helen and Hiblot, Julien and Griss, Rudolf and Johnsson, Kai
Journal: Methods in molecular biology (Clifton, N.J.) (2017): 101-117
Specifically and wash-free labeling of SNAP-tag fused proteins with a hybrid sensor to monitor local micro-viscosity.
Authors: Wang, Chao and Song, Xinbo and Chen, Lingcheng and Xiao, Yi
Journal: Biosensors & bioelectronics (2017): 313-320
mCarts: Genome-Wide Prediction of Clustered Sequence Motifs as Binding Sites for RNA-Binding Proteins.
Authors: Weyn-Vanhentenryck, Sebastien M and Zhang, Chaolin
Journal: Methods in molecular biology (Clifton, N.J.) (2016): 215-26
A Fluorescent Reporter for Single Cell Analysis of Gene Expression in Clostridium difficile.
Authors: Cassona, Carolina Piçarra and Pereira, Fátima and Serrano, Mónica and Henriques, Adriano O
Journal: Methods in molecular biology (Clifton, N.J.) (2016): 69-90
Dynamic internalization and recycling of a metal ion transporter: Cu homeostasis and CTR1, the human Cu⁺ uptake system.
Authors: Clifford, Rebecca J and Maryon, Edward B and Kaplan, Jack H
Journal: Journal of cell science (2016): 1711-21