BC Succinimidyl Ester
Ordering information
Price | |
Catalog Number | |
Unit Size | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 441.44 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Alternative formats
BC-Maleimide |
Overview | ![]() ![]() |
See also: Amine Reactive Dyes and Probes for Conjugation, Bioconjugation, Chemical Reagents, Dyes by Functional Group
Molecular weight 441.44 |
CLIP-tag is a modified version of SNAP-tag. It is also a self-labeling protein derived from human O6-alkylguanine-DNA-alkyltransferase. Instead of benzylguanine derivatives, CLIP tag is engineered to react with benzylcytosine derivatives. It can be used as a protein tag for tagging your protein of interest (POI). BC Succinimidyl Ester is an amine-reactive building block for preparing of SNAP substrates from amine-containing labels or biological targets. BC Succinimidyl Ester building block facilities the development of various CLIP-tag substrates for labeling CLIP-tag fusion proteins for a wide range of biological applications.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of BC Succinimidyl Ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 226.531 µL | 1.133 mL | 2.265 mL | 11.327 mL | 22.653 mL |
5 mM | 45.306 µL | 226.531 µL | 453.063 µL | 2.265 mL | 4.531 mL |
10 mM | 22.653 µL | 113.266 µL | 226.531 µL | 1.133 mL | 2.265 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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References
View all 38 references: Citation Explorer
Modulation of emission and singlet oxygen photosensitisation in live cells utilising bioorthogonal phosphorogenic probes and protein tag technology.
Authors: Leung, Peter Kam-Keung and Lo, Kenneth Kam-Wing
Journal: Chemical communications (Cambridge, England) (2020): 6074-6077
Authors: Leung, Peter Kam-Keung and Lo, Kenneth Kam-Wing
Journal: Chemical communications (Cambridge, England) (2020): 6074-6077
Expanding the substrate selectivity of SNAP/CLIP-tagging of intracellular targets.
Authors: Macias-Contreras, Miguel and Little, Kevin N and Zhu, Lei
Journal: Methods in enzymology (2020): 233-257
Authors: Macias-Contreras, Miguel and Little, Kevin N and Zhu, Lei
Journal: Methods in enzymology (2020): 233-257
Analysis of the nucleocytoplasmic shuttling RNA-binding protein HNRNPU using optimized HITS-CLIP method.
Authors: Yugami, Masato and Okano, Hideyuki and Nakanishi, Atsushi and Yano, Masato
Journal: PloS one (2020): e0231450
Authors: Yugami, Masato and Okano, Hideyuki and Nakanishi, Atsushi and Yano, Masato
Journal: PloS one (2020): e0231450
Visualization of Spirochetes by Labeling Membrane Proteins With Fluorescent Biarsenical Dyes.
Authors: Hillman, Chadwick and Stewart, Philip E and Strnad, Martin and Stone, Hunter and Starr, Tregei and Carmody, Aaron and Evans, Tyler J and Carracoi, Valentina and Wachter, Jenny and Rosa, Patricia A
Journal: Frontiers in cellular and infection microbiology (2019): 287
Authors: Hillman, Chadwick and Stewart, Philip E and Strnad, Martin and Stone, Hunter and Starr, Tregei and Carmody, Aaron and Evans, Tyler J and Carracoi, Valentina and Wachter, Jenny and Rosa, Patricia A
Journal: Frontiers in cellular and infection microbiology (2019): 287
Quantifying transcription factor binding dynamics at the single-molecule level in live cells.
Authors: Presman, Diego M and Ball, David A and Paakinaho, Ville and Grimm, Jonathan B and Lavis, Luke D and Karpova, Tatiana S and Hager, Gordon L
Journal: Methods (San Diego, Calif.) (2017): 76-88
Authors: Presman, Diego M and Ball, David A and Paakinaho, Ville and Grimm, Jonathan B and Lavis, Luke D and Karpova, Tatiana S and Hager, Gordon L
Journal: Methods (San Diego, Calif.) (2017): 76-88
Rational Design and Applications of Semisynthetic Modular Biosensors: SNIFITs and LUCIDs.
Authors: Farrants, Helen and Hiblot, Julien and Griss, Rudolf and Johnsson, Kai
Journal: Methods in molecular biology (Clifton, N.J.) (2017): 101-117
Authors: Farrants, Helen and Hiblot, Julien and Griss, Rudolf and Johnsson, Kai
Journal: Methods in molecular biology (Clifton, N.J.) (2017): 101-117
Specifically and wash-free labeling of SNAP-tag fused proteins with a hybrid sensor to monitor local micro-viscosity.
Authors: Wang, Chao and Song, Xinbo and Chen, Lingcheng and Xiao, Yi
Journal: Biosensors & bioelectronics (2017): 313-320
Authors: Wang, Chao and Song, Xinbo and Chen, Lingcheng and Xiao, Yi
Journal: Biosensors & bioelectronics (2017): 313-320
mCarts: Genome-Wide Prediction of Clustered Sequence Motifs as Binding Sites for RNA-Binding Proteins.
Authors: Weyn-Vanhentenryck, Sebastien M and Zhang, Chaolin
Journal: Methods in molecular biology (Clifton, N.J.) (2016): 215-26
Authors: Weyn-Vanhentenryck, Sebastien M and Zhang, Chaolin
Journal: Methods in molecular biology (Clifton, N.J.) (2016): 215-26
A Fluorescent Reporter for Single Cell Analysis of Gene Expression in Clostridium difficile.
Authors: Cassona, Carolina Piçarra and Pereira, Fátima and Serrano, Mónica and Henriques, Adriano O
Journal: Methods in molecular biology (Clifton, N.J.) (2016): 69-90
Authors: Cassona, Carolina Piçarra and Pereira, Fátima and Serrano, Mónica and Henriques, Adriano O
Journal: Methods in molecular biology (Clifton, N.J.) (2016): 69-90
Dynamic internalization and recycling of a metal ion transporter: Cu homeostasis and CTR1, the human Cu⁺ uptake system.
Authors: Clifford, Rebecca J and Maryon, Edward B and Kaplan, Jack H
Journal: Journal of cell science (2016): 1711-21
Authors: Clifford, Rebecca J and Maryon, Edward B and Kaplan, Jack H
Journal: Journal of cell science (2016): 1711-21
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)