BC Succinimidyl Ester

CLIP-tag is a modified version of SNAP-tag. It is also a self-labeling protein derived from human O6-alkylguanine-DNA-alkyltransferase. Instead of benzylguanine derivatives, CLIP tag is engineered to react with benzylcytosine derivatives. It can be used as a protein tag for tagging your protein of interest (POI). BC Succinimidyl Ester is an amine-reactive building block for preparing of SNAP substrates from amine-containing labels or biological targets. BC Succinimidyl Ester building block facilities the development of various CLIP-tag substrates for labeling CLIP-tag fusion proteins for a wide range of biological applications.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
12565	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of BC Succinimidyl Ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	226.531 µL	1.133 mL	2.265 mL	11.327 mL	22.653 mL
5 mM	45.306 µL	226.531 µL	453.063 µL	2.265 mL	4.531 mL
10 mM	22.653 µL	113.266 µL	226.531 µL	1.133 mL	2.265 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	441.44 g/mol	=	mL	x	mM	=	mmol

References

View all 38 references: Citation Explorer

Modulation of emission and singlet oxygen photosensitisation in live cells utilising bioorthogonal phosphorogenic probes and protein tag technology.

Authors:

Leung, Peter Kam-Keung and Lo, Kenneth Kam-Wing

Journal:

Chemical communications (Cambridge, England) (2020): 6074-6077

Expanding the substrate selectivity of SNAP/CLIP-tagging of intracellular targets.

Authors:

Macias-Contreras, Miguel and Little, Kevin N and Zhu, Lei

Journal:

Methods in enzymology (2020): 233-257

Analysis of the nucleocytoplasmic shuttling RNA-binding protein HNRNPU using optimized HITS-CLIP method.

Authors:

Yugami, Masato and Okano, Hideyuki and Nakanishi, Atsushi and Yano, Masato

Journal:

PloS one (2020): e0231450

Visualization of Spirochetes by Labeling Membrane Proteins With Fluorescent Biarsenical Dyes.

Authors:

Hillman, Chadwick and Stewart, Philip E and Strnad, Martin and Stone, Hunter and Starr, Tregei and Carmody, Aaron and Evans, Tyler J and Carracoi, Valentina and Wachter, Jenny and Rosa, Patricia A

Journal:

Frontiers in cellular and infection microbiology (2019): 287

Quantifying transcription factor binding dynamics at the single-molecule level in live cells.

Authors:

Presman, Diego M and Ball, David A and Paakinaho, Ville and Grimm, Jonathan B and Lavis, Luke D and Karpova, Tatiana S and Hager, Gordon L

Journal:

Methods (San Diego, Calif.) (2017): 76-88

Physical properties

Molecular weight	441.44
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on November 23, 2025