BC Succinimidyl Ester

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	441.44
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Alternative formats

BC-Maleimide

Overview SDS Protocol

Molecular weight

441.44

CLIP-tag is a modified version of SNAP-tag. It is also a self-labeling protein derived from human O6-alkylguanine-DNA-alkyltransferase. Instead of benzylguanine derivatives, CLIP tag is engineered to react with benzylcytosine derivatives. It can be used as a protein tag for tagging your protein of interest (POI). BC Succinimidyl Ester is an amine-reactive building block for preparing of SNAP substrates from amine-containing labels or biological targets. BC Succinimidyl Ester building block facilities the development of various CLIP-tag substrates for labeling CLIP-tag fusion proteins for a wide range of biological applications.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of BC Succinimidyl Ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	226.531 µL	1.133 mL	2.265 mL	11.327 mL	22.653 mL
5 mM	45.306 µL	226.531 µL	453.063 µL	2.265 mL	4.531 mL
10 mM	22.653 µL	113.266 µL	226.531 µL	1.133 mL	2.265 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Images

Figure 1. Chemical structure for BC Succinimidyl Ester.

References

View all 38 references: Citation Explorer

Modulation of emission and singlet oxygen photosensitisation in live cells utilising bioorthogonal phosphorogenic probes and protein tag technology.
Authors: Leung, Peter Kam-Keung and Lo, Kenneth Kam-Wing
Journal: Chemical communications (Cambridge, England) (2020): 6074-6077

Expanding the substrate selectivity of SNAP/CLIP-tagging of intracellular targets.
Authors: Macias-Contreras, Miguel and Little, Kevin N and Zhu, Lei
Journal: Methods in enzymology (2020): 233-257

Analysis of the nucleocytoplasmic shuttling RNA-binding protein HNRNPU using optimized HITS-CLIP method.
Authors: Yugami, Masato and Okano, Hideyuki and Nakanishi, Atsushi and Yano, Masato
Journal: PloS one (2020): e0231450

Visualization of Spirochetes by Labeling Membrane Proteins With Fluorescent Biarsenical Dyes.
Authors: Hillman, Chadwick and Stewart, Philip E and Strnad, Martin and Stone, Hunter and Starr, Tregei and Carmody, Aaron and Evans, Tyler J and Carracoi, Valentina and Wachter, Jenny and Rosa, Patricia A
Journal: Frontiers in cellular and infection microbiology (2019): 287

Quantifying transcription factor binding dynamics at the single-molecule level in live cells.
Authors: Presman, Diego M and Ball, David A and Paakinaho, Ville and Grimm, Jonathan B and Lavis, Luke D and Karpova, Tatiana S and Hager, Gordon L
Journal: Methods (San Diego, Calif.) (2017): 76-88

Rational Design and Applications of Semisynthetic Modular Biosensors: SNIFITs and LUCIDs.
Authors: Farrants, Helen and Hiblot, Julien and Griss, Rudolf and Johnsson, Kai
Journal: Methods in molecular biology (Clifton, N.J.) (2017): 101-117

Specifically and wash-free labeling of SNAP-tag fused proteins with a hybrid sensor to monitor local micro-viscosity.
Authors: Wang, Chao and Song, Xinbo and Chen, Lingcheng and Xiao, Yi
Journal: Biosensors & bioelectronics (2017): 313-320

mCarts: Genome-Wide Prediction of Clustered Sequence Motifs as Binding Sites for RNA-Binding Proteins.
Authors: Weyn-Vanhentenryck, Sebastien M and Zhang, Chaolin
Journal: Methods in molecular biology (Clifton, N.J.) (2016): 215-26

A Fluorescent Reporter for Single Cell Analysis of Gene Expression in Clostridium difficile.
Authors: Cassona, Carolina Piçarra and Pereira, Fátima and Serrano, Mónica and Henriques, Adriano O
Journal: Methods in molecular biology (Clifton, N.J.) (2016): 69-90

Dynamic internalization and recycling of a metal ion transporter: Cu homeostasis and CTR1, the human Cu⁺ uptake system.
Authors: Clifford, Rebecca J and Maryon, Edward B and Kaplan, Jack H
Journal: Journal of cell science (2016): 1711-21