iFluor® 790 succinimidyl ester

iFluor® 790 succinimidyl ester is an amine-reactive, near-infrared fluorescent dye designed for covalent labeling of proteins, particularly antibodies, for fluorescence imaging and detection applications.

Amine-Reactive Labeling: Covalently conjugates to primary amino groups of proteins.
Spectral Properties: Excitation/emission maxima of 787/812 nm.
Spectral Compatibility: Emission is well separated from Cy5, Cy7, and APC fluorophores.
Applications: Suitable for near-infrared animal imaging and two-color Western blot detection.

<p>HL-60 cells were incubated with (Red, +) or without (Green, -) Anti-human HLA-ABC (W6/32 mAb), followed by iFluor® 790 goat anti-mouse IgG conjugate. The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in APC-Cy7 channel.</p>

Protocol

Catalog	Size	Price
1368	1 mg	Price
71368	100 ug	Price
71524	5 mg	Price
71574	10 mg	Price

Overview

In vivo fluorescence imaging uses a sensitive camera to detect the fluorescence emission from fluorophores in whole-body living small animals. To overcome the photon attenuation in living tissue, fluorophores with long emission at the near-infrared (NIR) region are generally preferred, including widely used small indocarbocyanine dyes. Recent advances in imaging strategies and reporter techniques for in vivo fluorescence imaging include novel approaches to improve the specificity and affinity of the probes and to modulate and amplify the signal at target sites for enhanced sensitivity. Further emerging developments aim to achieve high-resolution, multimodality, and lifetime-based in vivo fluorescence imaging. Our iFluor® 790 is designed to label proteins and other biomolecules with near-infrared fluorescence. Conjugates prepared with iFluor® 790 have excitation and emission spectra similar to that of indocyanine green (ICG) and the IRDye® 800, with 787/812 nm excitation/emission maxima. iFluor® 790 dye emission is well separated from commonly used far-red fluorophores such as Cy5, Cy7, or allophycocyanin (APC), facilitating multicolor analysis. This fluorophore is also useful for small animal in-vivo imaging applications or other imaging applications requiring NIR detections, such as the two-color western applications with the LI-COR® Odyssey® infrared imaging system.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 790 succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	56.551 µL	282.757 µL	565.515 µL	2.828 mL	5.655 mL
5 mM	11.31 µL	56.551 µL	113.103 µL	565.515 µL	1.131 mL
10 mM	5.655 µL	28.276 µL	56.551 µL	282.757 µL	565.515 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1768.3 g/mol	=	mL	x	mM	=	mmol

Citations

View all 4 citations: Citation Explorer

Multifunctional lipid-based nanocarriers with antibacterial and anti-inflammatory activities for treating MRSA bacteremia in mice

Authors:

Liao, Chia-Chih and Yu, Huang-Ping and Yang, Shih-Chun and Alalaiwe, Ahmed and Dai, You-Shan and Liu, Fu-Chao and Fang, Jia-You

Journal:

Journal of Nanobiotechnology (2021): 1--18

Concentrated growth factor matrices prepared using silica-coated plastic tubes are distinguishable from those prepared using glass tubes in platelet distribution: Application of a novel near-infrared imaging-based, quantitative technique

Authors:

Yamaguchi, Sadahiro and Aizawa, Hachidai and Sato, Atsushi and Tsujino, Tetsuhiro and Isobe, Kazushige and Kitamura, Yutaka and Watanabe, Taisuke and Okudera, Hajime and Mour{\~a}o, Carlos Fernando and Kawase, Tomoyuki

Journal:

Frontiers in Bioengineering and Biotechnology (2020): 600

Squarticles as the nanoantidotes to sequester the overdosed antidepressant for detoxification

Authors:

Chen, Chun-Han and Huang, Tse-Hung and Elzoghby, Ahmed O and Wang, Pei-Wen and Chang, Chia-Wen and Fang, Jia-You

Journal:

International journal of nanomedicine (2017): 8071

Nanovesicle delivery to the liver via retinol binding protein and platelet-derived growth factor receptors: how targeting ligands affect biodistribution

Authors:

Hsu, Ching-Yun and Chen, Chun-Han and Aljuffali, Ibrahim A and Dai, You-Shan and Fang, Jia-You

Journal:

Nanomedicine (2017)

References

View all 18 references: Citation Explorer

In Vivo Fluorescence Microscopic Imaging for Dynamic Quantitative Assessment of Intestinal Mucosa Permeability in Mice

Authors:

Szabo A, Vollmar B, Boros M, Menger MD.

Journal:

J Surg Res. (2007)

A target cell-specific activatable fluorescence probe for in vivo molecular imaging of cancer based on a self-quenched avidin-rhodamine conjugate

Authors:

Hama Y, Urano Y, Koyama Y, Kamiya M, Bernardo M, Paik RS, Shin IS, Paik CH, Choyke PL, Kobayashi H.

Journal:

Cancer Res (2007): 2791

Fluorescence imaging in vivo: recent advances

Authors:

Rao J, Dragulescu-Andrasi A, Yao H.

Journal:

Curr Opin Biotechnol (2007): 17

Ex vivo fluorescence imaging of normal and malignant urothelial cells to enhance early diagnosis

Authors:

Steenkeste K, Lecart S, Deniset A, Pernot P, Eschwege P, Ferlicot S, Leveque-Fort S, Bri and et R, Fontaine-Aupart MP.

Journal:

Photochem Photobiol (2007): 1157

In vivo monitoring the fate of Cy5.5-Tat labeled T lymphocytes by quantitative near-infrared fluorescence imaging during acute brain inflammation in a rat model of experimental autoimmune encephalomyelitis

Authors:

Berger C, Gremlich HU, Schmidt P, Cannet C, Kneuer R, Hiest and P, Rausch M, Rudin M.

Journal:

J Immunol Methods (2007): 65

Physical properties

Molecular weight	1768.30
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.1
Correction factor (280 nm)	0.09
Extinction coefficient (cm ^-1 M ^-1)	250000 ¹
Excitation (nm)	787
Emission (nm)	812
Quantum yield	0.13 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on November 8, 2026