2',3'-cGAMP-Biotin conjugate

2',3'-cGAMP or cGAMP (Cyclic guanosine monophosphate“adenosine monophosphate) is produced in mammalian cells by cGAS (cGAMP synthase) from ATP and GTP upon cytosolic DNA stimulation. cGAMP produced by cGAS contains mixed phosphodiester linkages, with one between 2'-OH of GMP and 5'-phosphate of AMP and the other between 3'-OH of AMP and 5'-phosphate of GMP. 2',3'-cGAMP is also referred to as "noncanonical" cGAMP due to the presence of the atypical 2'-5' phosphodiester linkage between the guanosine and the adenosine. Similar to the canonical 3',3'-cGAMP, 2'3'-cGAMP serves as a second messenger to activate innate immune responses by binding to STING (stimulator of IFN genes) and subsequently inducing the TBK1-IRF3-dependent production of IFN-β. This biotin-labeled cGAMP provides an excellent probe for developing assays and test for detecting cGAMP and monitoring its activity.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
20316	100 ug	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 2',3'-cGAMP-Biotin conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	69.71 µL	348.551 µL	697.102 µL	3.486 mL	6.971 mL
5 mM	13.942 µL	69.71 µL	139.42 µL	697.102 µL	1.394 mL
10 mM	6.971 µL	34.855 µL	69.71 µL	348.551 µL	697.102 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1434.51 g/mol	=	mL	x	mM	=	mmol

Citations

View all 56 citations: Citation Explorer

African swine fever virus B175L inhibits the type I interferon pathway by targeting STING and 2′ 3′-cGAMP

Authors:

Ranathunga, Lakmal and Dodantenna, Niranjan and Cha, Ji-Won and Chathuranga, Kiramage and Chathuranga, WA Gayan and Weerawardhana, Asela and Subasinghe, Ashan and Haluwana, DK and Gamage, Nuwan and Lee, Jong-Soo

Journal:

Journal of Virology (2023): e00795--23

African Swine Fever Virus EP364R and C129R Target Cyclic GMP-AMP To Inhibit the cGAS-STING Signaling Pathway

Authors:

Dodantenna, Niranjan and Ranathunga, Lakmal and Chathuranga, WA Gayan and Weerawardhana, Asela and Cha, Ji-Won and Subasinghe, Ashan and Gamage, Nuwan and Haluwana, DK and Kim, YongKwan and Jheong, WeonHwa and others,

Journal:

Journal of Virology (2022): e01022--22

A Click-Chemistry Linked 2'3'-cGAMP Analogue

Authors:

Dialer, C. R., Stazzoni, S., Drexler, D. J., Muller, F. M., Veth, S., Pichler, A., Okamura, H., Witte, G., Hopfner, K. P., Carell, T.

Journal:

Chemistry (2019): 2089-2095

Viral and metazoan poxins are cGAMP-specific nucleases that restrict cGAS-STING signalling

Authors:

Eaglesham, J. B., Pan, Y., Kupper, T. S., Kranzusch, P. J.

Journal:

Nature (2019): 259-263

Structure and mechanism of a Hypr GGDEF enzyme that activates cGAMP signaling to control extracellular metal respiration

Authors:

Hallberg, Z. F., Chan, C. H., Wright, T. A., Kranzusch, P. J., Doxzen, K. W., Park, J. J., Bond, D. R., Hammond, M. C.

Journal:

Elife (2019): se name="20310.enl" path="C:\Users\aatbi\Drop

Physical properties

Molecular weight	1434.51
Solvent	DMSO

Storage, safety and handling

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 6, 2025