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2',3'-cGAMP-Biotin conjugate

Chemical structure for 2',3'-cGAMP-Biotin conjugate.
Chemical structure for 2',3'-cGAMP-Biotin conjugate.
Ordering information
Price ()
Catalog Number20316
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
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Physical properties
Molecular weight1434.51
SolventDMSO
Storage, safety and handling
Intended useResearch Use Only (RUO)
StorageFreeze (< -15 °C); Minimize light exposure
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OverviewpdfSDSpdfProtocol


Molecular weight
1434.51
2',3'-cGAMP or cGAMP (Cyclic guanosine monophosphate"adenosine monophosphate) is produced in mammalian cells by cGAS (cGAMP synthase) from ATP and GTP upon cytosolic DNA stimulation. cGAMP produced by cGAS contains mixed phosphodiester linkages, with one between 2\'-OH of GMP and 5\'-phosphate of AMP and the other between 3\'-OH of AMP and 5\'-phosphate of GMP. 2',3'-cGAMP is also referred to as "noncanonical" cGAMP due to the presence of the atypical 2'-5' phosphodiester linkage between the guanosine and the adenosine. Similar to the canonical 3',3'-cGAMP, 2'3'-cGAMP serves as a second messenger to activate innate immune responses by binding to STING (stimulator of IFN genes) and subsequently inducing the TBK1-IRF3-dependent production of IFN-β. This biotin-labeled cGAMP provides an excellent probe for developing assays and test for detecting cGAMP and monitoring its activity.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 2',3'-cGAMP-Biotin conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM69.71 µL348.551 µL697.102 µL3.486 mL6.971 mL
5 mM13.942 µL69.71 µL139.42 µL697.102 µL1.394 mL
10 mM6.971 µL34.855 µL69.71 µL348.551 µL697.102 µL

Molarity calculator

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Product family


Name
2',3'-cGAMP-Cy5 conjugate

Citations


View all 55 citations: Citation Explorer
African Swine Fever Virus EP364R and C129R Target Cyclic GMP-AMP To Inhibit the cGAS-STING Signaling Pathway
Authors: Dodantenna, Niranjan and Ranathunga, Lakmal and Chathuranga, WA Gayan and Weerawardhana, Asela and Cha, Ji-Won and Subasinghe, Ashan and Gamage, Nuwan and Haluwana, DK and Kim, YongKwan and Jheong, WeonHwa and others,
Journal: Journal of Virology (2022): e01022--22
The cGAS-cGAMP-STING Pathway: A Molecular Link Between Immunity and Metabolism
Authors: Bai, J., Liu, F.
Journal: Diabetes (2019): 1099-1108
Efficacy and mechanism of cGAMP to suppress Alzheimer's disease by elevating TREM2
Authors: Xu, Q., Xu, W., Cheng, H., Yuan, H., Tan, X.
Journal: Brain Behav Immun (2019): se name="20310.enl" path="C:\Users\aatbi\Drop
Discovery of a Novel cGAMP Competitive Ligand of the Inactive Form of STING
Authors: Siu, T., Altman, M. D., Baltus, G. A., Childers, M., Ellis, J. M., Gunaydin, H., Hatch, H., Ho, T., Jewell, J., Lacey, B. M., Lesburg, C. A., Pan, B. S., Sauvagnat, B., Schroeder, G. K., Xu, S.
Journal: ACS Med Chem Lett (2019): 92-97
Publisher Correction: Viral and metazoan poxins are cGAMP-specific nucleases that restrict cGAS-STING signalling
Authors: Eaglesham, J. B., Pan, Y., Kupper, T. S., Kranzusch, P. J.
Journal: Nature (2019): E12
The STING ligand cGAMP potentiates the efficacy of vaccine-induced CD8+ T cells
Authors: Gutjahr, A., Papagno, L., Nicoli, F., Kanuma, T., Kuse, N., Cabral-Piccin, M. P., Rochereau, N., Gostick, E., Lioux, T., Perouzel, E., Price, D. A., Takiguchi, M., Verrier, B., Yamamoto, T., Paul, S., Appay, V.
Journal: JCI Insight (2019): se name="20310.enl" path="C:\Users\aatbi\Drop
SLC19A1 Is an Importer of the Immunotransmitter cGAMP
Authors: Ritchie, C., Cordova, A. F., Hess, G. T., Bassik, M. C., Li, L.
Journal: Mol Cell (2019): 372-381 e5
A Click-Chemistry Linked 2'3'-cGAMP Analogue
Authors: Dialer, C. R., Stazzoni, S., Drexler, D. J., Muller, F. M., Veth, S., Pichler, A., Okamura, H., Witte, G., Hopfner, K. P., Carell, T.
Journal: Chemistry (2019): 2089-2095
Distinct Dynamic and Conformational Features of Human STING in Response to 2'3'-cGAMP and c-di-GMP
Authors: Guo, J., Wang, J., Fan, J., Zhang, Y., Dong, W., Chen, C. P.
Journal: Chembiochem (2019): 1838-1847
A Poxin on Both of Your Houses: Poxviruses Degrade the Immune Signal cGAMP
Authors: Dippel, A. B., Hammond, M. C.
Journal: Biochemistry (2019): 2387-2388