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2',3'-cGAMP-Cy5 conjugate

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Physical properties
Molecular weight1940.24
SolventDMSO
Storage, safety and handling
Intended useResearch Use Only (RUO)
StorageFreeze (< -15 °C); Minimize light exposure

OverviewpdfSDSpdfProtocol


Molecular weight
1940.24
2',3'-cGAMP or cGAMP (Cyclic guanosine monophosphate"adenosine monophosphate) is produced in mammalian cells by cGAS (cGAMP synthase) from ATP and GTP upon cytosolic DNA stimulation. cGAMP produced by cGAS contains mixed phosphodiester linkages, with one between 2\'-OH of GMP and 5\'-phosphate of AMP and the other between 3\'-OH of AMP and 5\'-phosphate of GMP. 2',3'-cGAMP is also referred to as "noncanonical" cGAMP due to the presence of the atypical 2'-5' phosphodiester linkage between the guanosine and the adenosine. Similar to the canonical 3',3'-cGAMP, 2'3'-cGAMP serves as a second messenger to activate innate immune responses by binding to STING (stimulator of IFN genes) and subsequently inducing the TBK1-IRF3-dependent production of IFN-β. This Cy5-labeled cGAMP provides an excellent probe for developing fluorescence-based assays and test for detecting cGAMP and monitoring its activity.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 2',3'-cGAMP-Cy5 conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM51.54 µL257.7 µL515.4 µL2.577 mL5.154 mL
5 mM10.308 µL51.54 µL103.08 µL515.4 µL1.031 mL
10 mM5.154 µL25.77 µL51.54 µL257.7 µL515.4 µL

Molarity calculator

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Citations


View all 55 citations: Citation Explorer
African Swine Fever Virus EP364R and C129R Target Cyclic GMP-AMP To Inhibit the cGAS-STING Signaling Pathway
Authors: Dodantenna, Niranjan and Ranathunga, Lakmal and Chathuranga, WA Gayan and Weerawardhana, Asela and Cha, Ji-Won and Subasinghe, Ashan and Gamage, Nuwan and Haluwana, DK and Kim, YongKwan and Jheong, WeonHwa and others,
Journal: Journal of Virology (2022): e01022--22
The cGAS-cGAMP-STING Pathway: A Molecular Link Between Immunity and Metabolism
Authors: Bai, J., Liu, F.
Journal: Diabetes (2019): 1099-1108
Efficacy and mechanism of cGAMP to suppress Alzheimer&apos;s disease by elevating TREM2
Authors: Xu, Q., Xu, W., Cheng, H., Yuan, H., Tan, X.
Journal: Brain Behav Immun (2019): se name="20310.enl" path="C:\Users\aatbi\Drop
Discovery of a Novel cGAMP Competitive Ligand of the Inactive Form of STING
Authors: Siu, T., Altman, M. D., Baltus, G. A., Childers, M., Ellis, J. M., Gunaydin, H., Hatch, H., Ho, T., Jewell, J., Lacey, B. M., Lesburg, C. A., Pan, B. S., Sauvagnat, B., Schroeder, G. K., Xu, S.
Journal: ACS Med Chem Lett (2019): 92-97
Publisher Correction: Viral and metazoan poxins are cGAMP-specific nucleases that restrict cGAS-STING signalling
Authors: Eaglesham, J. B., Pan, Y., Kupper, T. S., Kranzusch, P. J.
Journal: Nature (2019): E12
The STING ligand cGAMP potentiates the efficacy of vaccine-induced CD8+ T cells
Authors: Gutjahr, A., Papagno, L., Nicoli, F., Kanuma, T., Kuse, N., Cabral-Piccin, M. P., Rochereau, N., Gostick, E., Lioux, T., Perouzel, E., Price, D. A., Takiguchi, M., Verrier, B., Yamamoto, T., Paul, S., Appay, V.
Journal: JCI Insight (2019): se name="20310.enl" path="C:\Users\aatbi\Drop
SLC19A1 Is an Importer of the Immunotransmitter cGAMP
Authors: Ritchie, C., Cordova, A. F., Hess, G. T., Bassik, M. C., Li, L.
Journal: Mol Cell (2019): 372-381 e5
A Click-Chemistry Linked 2&apos;3&apos;-cGAMP Analogue
Authors: Dialer, C. R., Stazzoni, S., Drexler, D. J., Muller, F. M., Veth, S., Pichler, A., Okamura, H., Witte, G., Hopfner, K. P., Carell, T.
Journal: Chemistry (2019): 2089-2095
Distinct Dynamic and Conformational Features of Human STING in Response to 2&apos;3&apos;-cGAMP and c-di-GMP
Authors: Guo, J., Wang, J., Fan, J., Zhang, Y., Dong, W., Chen, C. P.
Journal: Chembiochem (2019): 1838-1847
A Poxin on Both of Your Houses: Poxviruses Degrade the Immune Signal cGAMP
Authors: Dippel, A. B., Hammond, M. C.
Journal: Biochemistry (2019): 2387-2388