2’,3’-cGAMP-iFluor 488 conjugate

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
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Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	1834.96
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.21
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Overview SDS Protocol

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 2’,3’-cGAMP-iFluor 488 conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	54.497 µL	272.486 µL	544.971 µL	2.725 mL	5.45 mL
5 mM	10.899 µL	54.497 µL	108.994 µL	544.971 µL	1.09 mL
10 mM	5.45 µL	27.249 µL	54.497 µL	272.486 µL	544.971 µL

Molarity calculator

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Spectrum

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Spectral properties

Correction Factor (260 nm)	0.21
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)	Correction Factor (656 nm)
2’,3’-cGAMP-iFluor 647 conjugate	656	670	250000¹	0.25¹	0.03	0.03	0.0793

Images

Figure 1. iFluor® 488-labeled cGAMP provides an excellent fluorescent probe for developing fluorescence-based assays and tests for detecting cGAMP or monitoring its activity. iFluor® 488 conjugates are significantly brighter than fluorescein conjugates and are much more photostable.

References

View all 49 references: Citation Explorer

Discovery of podofilox as a potent cGAMP-STING signaling enhancer with antitumor activity.
Authors: Zhang, Conggang and Han, Jing and Hu, Shuiqing and Hu, Yawei and Xu, Yifang and Hou, Yanfei and Yang, Yinlong and Su, Huili and Zhang, Zhengyin and Liu, Peng and Sun, Xuxu
Journal: Cancer immunology research (2023)

Improving poxvirus-mediated antitumor immune responses by deleting viral cGAMP-specific nuclease.
Authors: Riederer, Stephanie and Del Canizo, Ana and Navas, Javier and Peter, Marlowe G and Link, Ellen K and Sutter, Gerd and Rojas, Juan J
Journal: Cancer gene therapy (2023)

ESCRT-dependent STING degradation inhibits steady-state and cGAMP-induced signalling.
Authors: Gentili, Matteo and Liu, Bingxu and Papanastasiou, Malvina and Dele-Oni, Deborah and Schwartz, Marc A and Carlson, Rebecca J and Al'Khafaji, Aziz M and Krug, Karsten and Brown, Adam and Doench, John G and Carr, Steven A and Hacohen, Nir
Journal: Nature communications (2023): 611

Potent Therapeutic Strategies for COVID-19 with Single-Domain Antibody Immunoliposomes Neutralizing SARS-CoV-2 and Lip/cGAMP Enhancing Protective Immunity.
Authors: Zhou, Yajun and Lu, Xing and Wang, Xiaoqing and Ying, Tianlei and Tan, Xiangshi
Journal: International journal of molecular sciences (2023)

cGAMP-activated cGAS-STING signaling: its bacterial origins and evolutionary adaptation by metazoans.
Authors: Patel, Dinshaw J and Yu, You and Xie, Wei
Journal: Nature structural & molecular biology (2023): 245-260

Proteomic and phosphoproteomic analyses of Jurkat T-cell treated with 2'3' cGAMP reveals various signaling axes impacted by cyclic dinucleotides.
Authors: Onyedibe, Kenneth I and Mohallem, Rodrigo and Wang, Modi and Aryal, Uma K and Sintim, Herman O
Journal: Journal of proteomics (2023): 104869

STING agonist cGAMP enhances anti-tumor activity of CAR-NK cells against pancreatic cancer.
Authors: Da, Yanyan and Liu, Yuxia and Hu, Yuan and Liu, Wenzeng and Ma, Junpeng and Lu, Nan and Zhang, Chengsheng and Zhang, Cai
Journal: Oncoimmunology (2022): 2054105

LL-37 transports immunoreactive cGAMP to activate STING signaling and enhance interferon-mediated host antiviral immunity.
Authors: Wei, Xubiao and Zhang, Lulu and Yang, Yinlong and Hou, Yanfei and Xu, Yifang and Wang, Zhimeng and Su, Huili and Han, Fangping and Han, Jing and Liu, Peiyuan and Hu, Shuiqing and Koci, Matthew D and Sun, Xuxu and Zhang, Conggang
Journal: Cell reports (2022): 110880

cGAS Restricts PRRSV Replication by Sensing the mtDNA to Increase the cGAMP Activity.
Authors: Liu, Xiao-Na and Li, Li-Wei and Gao, Fei and Jiang, Yi-Feng and Yuan, Wan-Zhe and Li, Guo-Xin and Yu, Ling-Xue and Zhou, Yan-Jun and Tong, Guang-Zhi and Zhao, Kuan
Journal: Frontiers in immunology (2022): 887054

Production of 3',3'-cGAMP by a Bdellovibrio bacteriovorus promiscuous GGDEF enzyme, Bd0367, regulates exit from prey by gliding motility.
Authors: Lowry, Rebecca C and Hallberg, Zachary F and Till, Rob and Simons, Tyler J and Nottingham, Ruth and Want, Fiona and Sockett, R Elizabeth and Hammond, Ming C and Lambert, Carey
Journal: PLoS genetics (2022): e1010164