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2,3-Diaminonaphthalene *CAS 771-97-1*
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Application notes
The role of nitric oxide (NO) as a putative mediator of neuronal death can be understood best if NO is detected directly. 1,2-Diaminonaphthalene (DAN) is a fluorogenic probe that is used to detect nitric oxide (NO) productions in live cell and animals. The reaction product of DAN with NO is a triazole with blue fluorescence. The DAN-based methodology of NO assessment is convenient and applicable to numerous living systems both in vivo and in vitro. As low as 10 nM nitrite can be detected using DAN.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 2,3-Diaminonaphthalene *CAS 771-97-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 632.111 µL | 3.161 mL | 6.321 mL | 31.606 mL | 63.211 mL |
| 5 mM | 126.422 µL | 632.111 µL | 1.264 mL | 6.321 mL | 12.642 mL |
| 10 mM | 63.211 µL | 316.056 µL | 632.111 µL | 3.161 mL | 6.321 mL |
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References
View all 22 references: Citation Explorer
Immobilization and characterization of 2,3-diaminonaphthalene/cyclodextrin complexes in a sol-gel matrix: a new fluorimetric sensor for nitrite
Authors: Martinez-Tome MJ, Esquembre R, Mallavia R, Mateo CR.
Journal: J Fluoresc (2009): 119
Authors: Martinez-Tome MJ, Esquembre R, Mallavia R, Mateo CR.
Journal: J Fluoresc (2009): 119
Nitrogen oxide air pollutants interfere with the measurement of nitric oxide using 2,3-diaminonaphthalene: reduction of background interference
Authors: Fang YI, Hatori Y, Ohata H, Honda K.
Journal: Anal Biochem (2009): 132
Authors: Fang YI, Hatori Y, Ohata H, Honda K.
Journal: Anal Biochem (2009): 132
Fluorometric determination of nitrite with 2,3-diaminonaphthalene by reverse phase HPLC under alkaline conditions
Authors: Fang YI, Ohata H, Honda K.
Journal: J Pharmacol Toxicol Methods (2009): 153
Authors: Fang YI, Ohata H, Honda K.
Journal: J Pharmacol Toxicol Methods (2009): 153
Fluorometric measurement of nitrite/nitrate by 2,3-diaminonaphthalene
Authors: Nussler AK, Glanemann M, Schirmeier A, Liu L, Nussler NC.
Journal: Nat Protoc (2006): 2223
Authors: Nussler AK, Glanemann M, Schirmeier A, Liu L, Nussler NC.
Journal: Nat Protoc (2006): 2223
Determination of selenium in human serum by liquid chromatography/electron capture atmospheric pressure chemical ionization mass spectrometry after acid digestion and derivatization using 2,3-diaminonaphthalene
Authors: Ando M, Takizawa M, Suwabe S, Yamato S, Shimada K.
Journal: Eur J Mass Spectrom (Chichester, Eng) (2003): 619
Authors: Ando M, Takizawa M, Suwabe S, Yamato S, Shimada K.
Journal: Eur J Mass Spectrom (Chichester, Eng) (2003): 619