AAT Bioquest

2,3-Diaminonaphthalene *CAS 771-97-1*

Product Image
Product Image
Gallery Image 1
Ordering information
Catalog Number
Unit Size
Add to cart
Additional ordering information
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
Request quotation
Physical properties
Molecular weight158.20
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
The role of nitric oxide (NO) as a putative mediator of neuronal death can be understood best if NO is detected directly. 1,2-Diaminonaphthalene (DAN) is a fluorogenic probe that is used to detect nitric oxide (NO) productions in live cell and animals. The reaction product of DAN with NO is a triazole with blue fluorescence. The DAN-based methodology of NO assessment is convenient and applicable to numerous living systems both in vivo and in vitro. As low as 10 nM nitrite can be detected using DAN.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 2,3-Diaminonaphthalene *CAS 771-97-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM632.111 µL3.161 mL6.321 mL31.606 mL63.211 mL
5 mM126.422 µL632.111 µL1.264 mL6.321 mL12.642 mL
10 mM63.211 µL316.056 µL632.111 µL3.161 mL6.321 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles



View all 22 references: Citation Explorer
Immobilization and characterization of 2,3-diaminonaphthalene/cyclodextrin complexes in a sol-gel matrix: a new fluorimetric sensor for nitrite
Authors: Martinez-Tome MJ, Esquembre R, Mallavia R, Mateo CR.
Journal: J Fluoresc (2009): 119
Nitrogen oxide air pollutants interfere with the measurement of nitric oxide using 2,3-diaminonaphthalene: reduction of background interference
Authors: Fang YI, Hatori Y, Ohata H, Honda K.
Journal: Anal Biochem (2009): 132
Fluorometric determination of nitrite with 2,3-diaminonaphthalene by reverse phase HPLC under alkaline conditions
Authors: Fang YI, Ohata H, Honda K.
Journal: J Pharmacol Toxicol Methods (2009): 153
Fluorometric measurement of nitrite/nitrate by 2,3-diaminonaphthalene
Authors: Nussler AK, Glanemann M, Schirmeier A, Liu L, Nussler NC.
Journal: Nat Protoc (2006): 2223
Determination of selenium in human serum by liquid chromatography/electron capture atmospheric pressure chemical ionization mass spectrometry after acid digestion and derivatization using 2,3-diaminonaphthalene
Authors: Ando M, Takizawa M, Suwabe S, Yamato S, Shimada K.
Journal: Eur J Mass Spectrom (Chichester, Eng) (2003): 619
Study on the molecular recognitions of calix[n]arenes to 2,3-diaminonaphthalene by using fluorometric technique
Authors: Yang J, Huang F, Wang M, Wu X, Sun C.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2002): 1775
A simple HPLC-fluorescence detection of nitric oxide in cultivated plant cells by in situ derivatization with 2,3-diaminonaphthalene
Authors: Wada M, Morinaka C, Ikenaga T, Kuroda N, Nakashima K.
Journal: Anal Sci (2002): 631
Sources of interference in the use of 2,3-diaminonaphthalene for the fluorimetric determination of nitric oxide synthase activity in biological samples
Authors: Fern, undefined and ez-Cancio M, Fern and ez-Vitos EM, Centelles JJ, Imperial S.
Journal: Clin Chim Acta (2001): 205
Successive determinations of platinum(II) and selenium(IV) with 1,4-dibromo-2,3-diaminonaphthalene in aqueous micellar solutions
Authors: Warashina T, Hoshino H, Yotsuyanagi T.
Journal: Anal Sci (2001): 859
Fluorescent derivatization of nitrite ions with 2,3-diaminonaphthalene utilizing a pH gradient in a Y-shaped microchannel
Authors: Odake T, Tabuchi M, Sato T, Susaki H, Korenaga T.
Journal: Anal Sci (2001): 535