2,3-Diaminonaphthalene *CAS 771-97-1*

2,3-Diaminonaphthalene

CAS 771-97-1

The role of nitric oxide (NO) as a putative mediator of neuronal death can be understood best if NO is detected directly. 1,2-Diaminonaphthalene (DAN) is a fluorogenic probe that is used to detect nitric oxide (NO) productions in live cell and animals. The reaction product of DAN with NO is a triazole with blue fluorescence. The DAN-based methodology of NO assessment is convenient and applicable to numerous living systems both in vivo and in vitro. As low as 10 nM nitrite can be detected using DAN.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
15221	100 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 2,3-Diaminonaphthalene *CAS 771-97-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	632.111 µL	3.161 mL	6.321 mL	31.606 mL	63.211 mL
5 mM	126.422 µL	632.111 µL	1.264 mL	6.321 mL	12.642 mL
10 mM	63.211 µL	316.056 µL	632.111 µL	3.161 mL	6.321 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	158.2 g/mol	=	mL	x	mM	=	mmol

References

View all 22 references: Citation Explorer

Immobilization and characterization of 2,3-diaminonaphthalene/cyclodextrin complexes in a sol-gel matrix: a new fluorimetric sensor for nitrite

Authors:

Martinez-Tome MJ, Esquembre R, Mallavia R, Mateo CR.

Journal:

J Fluoresc (2009): 119

Nitrogen oxide air pollutants interfere with the measurement of nitric oxide using 2,3-diaminonaphthalene: reduction of background interference

Authors:

Fang YI, Hatori Y, Ohata H, Honda K.

Journal:

Anal Biochem (2009): 132

Fluorometric determination of nitrite with 2,3-diaminonaphthalene by reverse phase HPLC under alkaline conditions

Authors:

Fang YI, Ohata H, Honda K.

Journal:

J Pharmacol Toxicol Methods (2009): 153

Fluorometric measurement of nitrite/nitrate by 2,3-diaminonaphthalene

Authors:

Nussler AK, Glanemann M, Schirmeier A, Liu L, Nussler NC.

Journal:

Nat Protoc (2006): 2223

Determination of selenium in human serum by liquid chromatography/electron capture atmospheric pressure chemical ionization mass spectrometry after acid digestion and derivatization using 2,3-diaminonaphthalene

Authors:

Ando M, Takizawa M, Suwabe S, Yamato S, Shimada K.

Journal:

Eur J Mass Spectrom (Chichester, Eng) (2003): 619

Physical properties

Molecular weight	158.20
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	771-97-1

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 22, 2025