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2,3-Diaminonaphthalene *CAS 771-97-1*
Ordering information
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Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
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| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 158.20 |
| Solvent | DMSO |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12352200 |
| Overview |  SDSProtocol |
See also: Reactive Oxygen Species (ROS)
CAS 771-97-1 | Molecular weight 158.20 |
The role of nitric oxide (NO) as a putative mediator of neuronal death can be understood best if NO is detected directly. 1,2-Diaminonaphthalene (DAN) is a fluorogenic probe that is used to detect nitric oxide (NO) productions in live cell and animals. The reaction product of DAN with NO is a triazole with blue fluorescence. The DAN-based methodology of NO assessment is convenient and applicable to numerous living systems both in vivo and in vitro. As low as 10 nM nitrite can be detected using DAN.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 2,3-Diaminonaphthalene *CAS 771-97-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 632.111 µL | 3.161 mL | 6.321 mL | 31.606 mL | 63.211 mL |
| 5 mM | 126.422 µL | 632.111 µL | 1.264 mL | 6.321 mL | 12.642 mL |
| 10 mM | 63.211 µL | 316.056 µL | 632.111 µL | 3.161 mL | 6.321 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Images
Figure 1. Chemical structure for 2,3-Diaminonaphthalene *CAS 771-97-1*
References
View all 22 references: Citation Explorer
Immobilization and characterization of 2,3-diaminonaphthalene/cyclodextrin complexes in a sol-gel matrix: a new fluorimetric sensor for nitrite
Authors: Martinez-Tome MJ, Esquembre R, Mallavia R, Mateo CR.
Journal: J Fluoresc (2009): 119
Authors: Martinez-Tome MJ, Esquembre R, Mallavia R, Mateo CR.
Journal: J Fluoresc (2009): 119
Nitrogen oxide air pollutants interfere with the measurement of nitric oxide using 2,3-diaminonaphthalene: reduction of background interference
Authors: Fang YI, Hatori Y, Ohata H, Honda K.
Journal: Anal Biochem (2009): 132
Authors: Fang YI, Hatori Y, Ohata H, Honda K.
Journal: Anal Biochem (2009): 132
Fluorometric determination of nitrite with 2,3-diaminonaphthalene by reverse phase HPLC under alkaline conditions
Authors: Fang YI, Ohata H, Honda K.
Journal: J Pharmacol Toxicol Methods (2009): 153
Authors: Fang YI, Ohata H, Honda K.
Journal: J Pharmacol Toxicol Methods (2009): 153
Fluorometric measurement of nitrite/nitrate by 2,3-diaminonaphthalene
Authors: Nussler AK, Glanemann M, Schirmeier A, Liu L, Nussler NC.
Journal: Nat Protoc (2006): 2223
Authors: Nussler AK, Glanemann M, Schirmeier A, Liu L, Nussler NC.
Journal: Nat Protoc (2006): 2223
Determination of selenium in human serum by liquid chromatography/electron capture atmospheric pressure chemical ionization mass spectrometry after acid digestion and derivatization using 2,3-diaminonaphthalene
Authors: Ando M, Takizawa M, Suwabe S, Yamato S, Shimada K.
Journal: Eur J Mass Spectrom (Chichester, Eng) (2003): 619
Authors: Ando M, Takizawa M, Suwabe S, Yamato S, Shimada K.
Journal: Eur J Mass Spectrom (Chichester, Eng) (2003): 619
Study on the molecular recognitions of calix[n]arenes to 2,3-diaminonaphthalene by using fluorometric technique
Authors: Yang J, Huang F, Wang M, Wu X, Sun C.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2002): 1775
Authors: Yang J, Huang F, Wang M, Wu X, Sun C.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2002): 1775
A simple HPLC-fluorescence detection of nitric oxide in cultivated plant cells by in situ derivatization with 2,3-diaminonaphthalene
Authors: Wada M, Morinaka C, Ikenaga T, Kuroda N, Nakashima K.
Journal: Anal Sci (2002): 631
Authors: Wada M, Morinaka C, Ikenaga T, Kuroda N, Nakashima K.
Journal: Anal Sci (2002): 631
Sources of interference in the use of 2,3-diaminonaphthalene for the fluorimetric determination of nitric oxide synthase activity in biological samples
Authors: Fern, undefined and ez-Cancio M, Fern and ez-Vitos EM, Centelles JJ, Imperial S.
Journal: Clin Chim Acta (2001): 205
Authors: Fern, undefined and ez-Cancio M, Fern and ez-Vitos EM, Centelles JJ, Imperial S.
Journal: Clin Chim Acta (2001): 205
Successive determinations of platinum(II) and selenium(IV) with 1,4-dibromo-2,3-diaminonaphthalene in aqueous micellar solutions
Authors: Warashina T, Hoshino H, Yotsuyanagi T.
Journal: Anal Sci (2001): 859
Authors: Warashina T, Hoshino H, Yotsuyanagi T.
Journal: Anal Sci (2001): 859
Fluorescent derivatization of nitrite ions with 2,3-diaminonaphthalene utilizing a pH gradient in a Y-shaped microchannel
Authors: Odake T, Tabuchi M, Sato T, Susaki H, Korenaga T.
Journal: Anal Sci (2001): 535
Authors: Odake T, Tabuchi M, Sato T, Susaki H, Korenaga T.
Journal: Anal Sci (2001): 535
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FAQ
How do you prepare a stock solution of 2,3-Diaminonapthalene?
Why should I use an absorbance ratio at A575nm/A605nm when using most of your Amplite® Colorimetric Assay Kits?
How should I reconstitute an NADPH standard?
How can I lyse my cells without lysing the nuclear membrane?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
Why should I use an absorbance ratio at A575nm/A605nm when using most of your Amplite® Colorimetric Assay Kits?
How should I reconstitute an NADPH standard?
How can I lyse my cells without lysing the nuclear membrane?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?