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DNP-X acid, SE [6-(2,4-Dinitrophenyl)aminohexanoic acid, succinimidyl ester] *CAS 82321-04-8*

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Physical properties
Molecular weight394.34
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

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CAS
82321-04-8
Molecular weight
394.34
An amine-reactive building block for developing a probe that can be recognized by anti-DNP antibodies; Also an excellent amine-reactive FRET quencher paired with Trp or Tyr.

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Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DNP-X acid, SE [6-(2,4-Dinitrophenyl)aminohexanoic acid, succinimidyl ester] *CAS 82321-04-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM253.588 µL1.268 mL2.536 mL12.679 mL25.359 mL
5 mM50.718 µL253.588 µL507.177 µL2.536 mL5.072 mL
10 mM25.359 µL126.794 µL253.588 µL1.268 mL2.536 mL

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References


View all 124 references: Citation Explorer
Continuous assay of protein tyrosine phosphatases based on fluorescence resonance energy transfer
Authors: Nishikata, M.; Yoshimura, Y.; Deyama, Y.; Suzuki, K.
Journal: Biochimie (2006): 879-86
High-throughput screening of low molecular weight NS3-NS4A protease inhibitors using a fluorescence resonance energy transfer substrate
Authors: Sudo K, Yamaji K, Kawamura K, Nishijima T, Kojima N, Aibe K, Shimotohno K, Shimizu Y.
Journal: Antivir Chem Chemother (2005): 385
Recombinant human cathepsin X is a carboxymonopeptidase only: a comparison with cathepsins B and L
Authors: Puzer L, Cotrin SS, Cezari MH, Hirata IY, Juliano MA, Stefe I, Turk D, Turk B, Juliano L, Carmona AK.
Journal: Biol Chem (2005): 1191
Evaluation of an imaging platform during the development of a FRET protease assay
Authors: George J, Teear ML, Norey CG, Burns DD.
Journal: J Biomol Screen (2003): 72
Generation of heterogeneous rabbit anti-DNP antibodies by gene conversion and hypermutation of rearranged VL and VH genes during clonal expansion of B cells in splenic germinal centers
Authors: Sehgal D, Schiaffella E, Anderson AO, Mage RG.
Journal: Eur J Immunol (2000): 3634
Effects and mechanisms of environmental temperature on carp (Cyprinus carpio) anti-DNP antibody response and non-specific cytotoxic cell activity: a kinetic study
Authors: Le Morvan C, Deschaux P, Troutaud D.
Journal: Dev Comp Immunol (1996): 331
Determination of the binding of ligands containing the N-2,4-dinitrophenyl group to bivalent monoclonal rat anti-DNP antibody using affinity capillary electrophoresis
Authors: Mammen M, Gomez FA, Whitesides GM.
Journal: Anal Chem (1995): 3526
Effects of Celosia argentea and Cucurbita moschata extracts on anti-DNP IgE antibody production in mice
Authors: Imaoka K, Ushijima H, Inouye S, Takahashi T, Kojima Y.
Journal: Arerugi (1994): 652
Effects of Perilla frutescens extract on anti-DNP IgE antibody production in mice
Authors: Imaoka K, Inouye S, Takahashi T, Kojima Y.
Journal: Arerugi (1993): 74
Effect of anti-DNP IgG1- and IgG2a-secreting hybridomas in vivo on the development of an anti-DNP IgE antibody response in mice
Authors: Hagen M, Morrison B, Robbinson D, Strejan GH.
Journal: Int Arch Allergy Immunol (1992): 146