DNP-X acid, SE [6-(2,4-Dinitrophenyl)aminohexanoic acid, succinimidyl ester] *CAS 82321-04-8*

DNP-X acid, SE

6-(2,4-Dinitrophenyl)aminohexanoic acid, succinimidyl ester; CAS 82321-04-8

An amine-reactive building block for developing a probe that can be recognized by anti-DNP antibodies; Also an excellent amine-reactive FRET quencher paired with Trp or Tyr.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
2021	25 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DNP-X acid, SE [6-(2,4-Dinitrophenyl)aminohexanoic acid, succinimidyl ester] *CAS 82321-04-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	253.588 µL	1.268 mL	2.536 mL	12.679 mL	25.359 mL
5 mM	50.718 µL	253.588 µL	507.177 µL	2.536 mL	5.072 mL
10 mM	25.359 µL	126.794 µL	253.588 µL	1.268 mL	2.536 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	394.34 g/mol	=	mL	x	mM	=	mmol

References

View all 124 references: Citation Explorer

Continuous assay of protein tyrosine phosphatases based on fluorescence resonance energy transfer

Authors:

Nishikata, M.; Yoshimura, Y.; Deyama, Y.; Suzuki, K.

Journal:

Biochimie (2006): 879-86

High-throughput screening of low molecular weight NS3-NS4A protease inhibitors using a fluorescence resonance energy transfer substrate

Authors:

Sudo K, Yamaji K, Kawamura K, Nishijima T, Kojima N, Aibe K, Shimotohno K, Shimizu Y.

Journal:

Antivir Chem Chemother (2005): 385

Recombinant human cathepsin X is a carboxymonopeptidase only: a comparison with cathepsins B and L

Authors:

Puzer L, Cotrin SS, Cezari MH, Hirata IY, Juliano MA, Stefe I, Turk D, Turk B, Juliano L, Carmona AK.

Journal:

Biol Chem (2005): 1191

Evaluation of an imaging platform during the development of a FRET protease assay

Authors:

George J, Teear ML, Norey CG, Burns DD.

Journal:

J Biomol Screen (2003): 72

Generation of heterogeneous rabbit anti-DNP antibodies by gene conversion and hypermutation of rearranged VL and VH genes during clonal expansion of B cells in splenic germinal centers

Authors:

Sehgal D, Schiaffella E, Anderson AO, Mage RG.

Journal:

Eur J Immunol (2000): 3634

Physical properties

Molecular weight	394.34
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	82321-04-8

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