DNP-PEG4 NHS ester

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Physical properties

Molecular weight	528.47
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

858126-78-0

Molecular weight

528.47

Compared to the commonly used DNP-X SE (#2021), DNP-PEG4 NHS ester has much better water solubility. It is an excellent amine-reactive building block for developing DNP probes that can be recognized by anti-DNP antibodies. It might be also used as an excellent amine-reactive FRET quencher paired with Trp or Tyr. Its terminal NHS ester group readily reacts with primary amines to form a stable amide bond. The hydrophilic PEG linker increases enable increasing number of DNP groups conjugated to a biological molecule without causing precipitation.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DNP-PEG4 NHS ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	189.226 µL	946.128 µL	1.892 mL	9.461 mL	18.923 mL
5 mM	37.845 µL	189.226 µL	378.451 µL	1.892 mL	3.785 mL
10 mM	18.923 µL	94.613 µL	189.226 µL	946.128 µL	1.892 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Images

Figure 1. Chemical structure for DNP-PEG4 NHS ester.

References

View all 50 references: Citation Explorer

Preventative and Therapeutic Effects of Low-dose Ionizing Radiation on the Allergic Response of Rat Basophilic Leukemia Cells.
Authors: Joo, Hae Mi and Hong, Eun Hee and Cho, Seong-Jun and Nam, Seon Young and Kim, Ji Young
Journal: Scientific reports (2019): 16079

Age-associated oxidative modifications of mitochondrial α-subunit of F1 ATP synthase from mouse skeletal muscles.
Authors: Das, N and Jana, C K
Journal: Free radical research (2015): 954-61

Rapid desensitization induces internalization of antigen-specific IgE on mouse mast cells.
Authors: Oka, Tatsuya and Rios, Eon J and Tsai, Mindy and Kalesnikoff, Janet and Galli, Stephen J
Journal: The Journal of allergy and clinical immunology (2013): 922-32.e1-16

IgG immune complex induces the recruitment of inflammatory cells into the airway and TNF-mediated late airway hyperresponsiveness via NF-κB activation in mice.
Authors: Lee, Chang-Hoon and Kim, Young-Suk and Kang, Nam-In and Lee, Young-Man and Kim, Kyoung-Jin and Chai, Ok-Hee and Song, Chang-Ho and Kim, Hae-Kyoung and Im, Suhn-Young and Oh, Dae-Kyu and Lee, Hern-Ku
Journal: The Journal of asthma : official journal of the Association for the Care of Asthma (2011): 757-66

Detection and localization of markers of oxidative stress by in situ methods: application in the study of Alzheimer disease.
Authors: Moreira, Paula I and Sayre, Lawrence M and Zhu, Xiongwei and Nunomura, Akihiko and Smith, Mark A and Perry, George
Journal: Methods in molecular biology (Clifton, N.J.) (2010): 419-34

A non-chromatographic method for the purification of a bivalently active monoclonal IgG antibody from biological fluids.
Authors: Bilgiçer, Başar and Thomas, Samuel W and Shaw, Bryan F and Kaufman, George K and Krishnamurthy, Vijay M and Estroff, Lara A and Yang, Jerry and Whitesides, George M
Journal: Journal of the American Chemical Society (2009): 9361-7

An antibody-recruiting small molecule that targets HIV gp120.
Authors: Parker, Christopher G and Domaoal, Robert A and Anderson, Karen S and Spiegel, David A
Journal: Journal of the American Chemical Society (2009): 16392-4

Mutation of superoxide dismutase elevates reactive species: comparison of nitration and oxidation of proteins in different brain regions of transgenic mice with amyotrophic lateral sclerosis.
Authors: Liu, D and Bao, F and Wen, J and Liu, J
Journal: Neuroscience (2007): 255-64

Nonprotein-bound iron and plasma protein oxidative stress at birth.
Authors: Marzocchi, Barbara and Perrone, Serafina and Paffetti, Patrizia and Magi, Barbara and Bini, Luca and Tani, Chiara and Longini, Mariangela and Buonocore, Giuseppe
Journal: Pediatric research (2005): 1295-9

Marginal zone B cell enrichment and strong follicular B cell reduction correlate with a delayed IgG response in a light chain diversity restricted mouse model.
Authors: Amrani, Yacine M and Voegtlé, Danielle and Barbier, Eliane and Six, Adrien and Cazenave, Pierre-André
Journal: European journal of immunology (2004): 2812-23