DNP-PEG4 NHS ester
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 528.47 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
CAS 858126-78-0 | Molecular weight 528.47 |
Compared to the commonly used DNP-X SE (#2021), DNP-PEG4 NHS ester has much better water solubility. It is an excellent amine-reactive building block for developing DNP probes that can be recognized by anti-DNP antibodies. It might be also used as an excellent amine-reactive FRET quencher paired with Trp or Tyr. Its terminal NHS ester group readily reacts with primary amines to form a stable amide bond. The hydrophilic PEG linker increases enable increasing number of DNP groups conjugated to a biological molecule without causing precipitation.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DNP-PEG4 NHS ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 189.226 µL | 946.128 µL | 1.892 mL | 9.461 mL | 18.923 mL |
5 mM | 37.845 µL | 189.226 µL | 378.451 µL | 1.892 mL | 3.785 mL |
10 mM | 18.923 µL | 94.613 µL | 189.226 µL | 946.128 µL | 1.892 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
References
View all 50 references: Citation Explorer
Preventative and Therapeutic Effects of Low-dose Ionizing Radiation on the Allergic Response of Rat Basophilic Leukemia Cells.
Authors: Joo, Hae Mi and Hong, Eun Hee and Cho, Seong-Jun and Nam, Seon Young and Kim, Ji Young
Journal: Scientific reports (2019): 16079
Authors: Joo, Hae Mi and Hong, Eun Hee and Cho, Seong-Jun and Nam, Seon Young and Kim, Ji Young
Journal: Scientific reports (2019): 16079
Age-associated oxidative modifications of mitochondrial α-subunit of F1 ATP synthase from mouse skeletal muscles.
Authors: Das, N and Jana, C K
Journal: Free radical research (2015): 954-61
Authors: Das, N and Jana, C K
Journal: Free radical research (2015): 954-61
Rapid desensitization induces internalization of antigen-specific IgE on mouse mast cells.
Authors: Oka, Tatsuya and Rios, Eon J and Tsai, Mindy and Kalesnikoff, Janet and Galli, Stephen J
Journal: The Journal of allergy and clinical immunology (2013): 922-32.e1-16
Authors: Oka, Tatsuya and Rios, Eon J and Tsai, Mindy and Kalesnikoff, Janet and Galli, Stephen J
Journal: The Journal of allergy and clinical immunology (2013): 922-32.e1-16
IgG immune complex induces the recruitment of inflammatory cells into the airway and TNF-mediated late airway hyperresponsiveness via NF-κB activation in mice.
Authors: Lee, Chang-Hoon and Kim, Young-Suk and Kang, Nam-In and Lee, Young-Man and Kim, Kyoung-Jin and Chai, Ok-Hee and Song, Chang-Ho and Kim, Hae-Kyoung and Im, Suhn-Young and Oh, Dae-Kyu and Lee, Hern-Ku
Journal: The Journal of asthma : official journal of the Association for the Care of Asthma (2011): 757-66
Authors: Lee, Chang-Hoon and Kim, Young-Suk and Kang, Nam-In and Lee, Young-Man and Kim, Kyoung-Jin and Chai, Ok-Hee and Song, Chang-Ho and Kim, Hae-Kyoung and Im, Suhn-Young and Oh, Dae-Kyu and Lee, Hern-Ku
Journal: The Journal of asthma : official journal of the Association for the Care of Asthma (2011): 757-66
Detection and localization of markers of oxidative stress by in situ methods: application in the study of Alzheimer disease.
Authors: Moreira, Paula I and Sayre, Lawrence M and Zhu, Xiongwei and Nunomura, Akihiko and Smith, Mark A and Perry, George
Journal: Methods in molecular biology (Clifton, N.J.) (2010): 419-34
Authors: Moreira, Paula I and Sayre, Lawrence M and Zhu, Xiongwei and Nunomura, Akihiko and Smith, Mark A and Perry, George
Journal: Methods in molecular biology (Clifton, N.J.) (2010): 419-34
A non-chromatographic method for the purification of a bivalently active monoclonal IgG antibody from biological fluids.
Authors: Bilgiçer, Başar and Thomas, Samuel W and Shaw, Bryan F and Kaufman, George K and Krishnamurthy, Vijay M and Estroff, Lara A and Yang, Jerry and Whitesides, George M
Journal: Journal of the American Chemical Society (2009): 9361-7
Authors: Bilgiçer, Başar and Thomas, Samuel W and Shaw, Bryan F and Kaufman, George K and Krishnamurthy, Vijay M and Estroff, Lara A and Yang, Jerry and Whitesides, George M
Journal: Journal of the American Chemical Society (2009): 9361-7
An antibody-recruiting small molecule that targets HIV gp120.
Authors: Parker, Christopher G and Domaoal, Robert A and Anderson, Karen S and Spiegel, David A
Journal: Journal of the American Chemical Society (2009): 16392-4
Authors: Parker, Christopher G and Domaoal, Robert A and Anderson, Karen S and Spiegel, David A
Journal: Journal of the American Chemical Society (2009): 16392-4
Mutation of superoxide dismutase elevates reactive species: comparison of nitration and oxidation of proteins in different brain regions of transgenic mice with amyotrophic lateral sclerosis.
Authors: Liu, D and Bao, F and Wen, J and Liu, J
Journal: Neuroscience (2007): 255-64
Authors: Liu, D and Bao, F and Wen, J and Liu, J
Journal: Neuroscience (2007): 255-64
Nonprotein-bound iron and plasma protein oxidative stress at birth.
Authors: Marzocchi, Barbara and Perrone, Serafina and Paffetti, Patrizia and Magi, Barbara and Bini, Luca and Tani, Chiara and Longini, Mariangela and Buonocore, Giuseppe
Journal: Pediatric research (2005): 1295-9
Authors: Marzocchi, Barbara and Perrone, Serafina and Paffetti, Patrizia and Magi, Barbara and Bini, Luca and Tani, Chiara and Longini, Mariangela and Buonocore, Giuseppe
Journal: Pediatric research (2005): 1295-9
Marginal zone B cell enrichment and strong follicular B cell reduction correlate with a delayed IgG response in a light chain diversity restricted mouse model.
Authors: Amrani, Yacine M and Voegtlé, Danielle and Barbier, Eliane and Six, Adrien and Cazenave, Pierre-André
Journal: European journal of immunology (2004): 2812-23
Authors: Amrani, Yacine M and Voegtlé, Danielle and Barbier, Eliane and Six, Adrien and Cazenave, Pierre-André
Journal: European journal of immunology (2004): 2812-23