Nile Red *CAS 7385-67-3*
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Physical properties
Molecular weight | 318.37 |
Solvent | DMSO |
Spectral properties
Absorbance (nm) | 560 |
Extinction coefficient (cm -1 M -1) | 38000 |
Excitation (nm) | 559 |
Emission (nm) | 635 |
Quantum yield | 0.70001 |
Storage, safety and handling
Certificate of Origin | Download PDF |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | SDSProtocol |
CAS 7385-67-3 | Molecular weight 318.37 | Absorbance (nm) 560 | Extinction coefficient (cm -1 M -1) 38000 | Excitation (nm) 559 | Emission (nm) 635 | Quantum yield 0.70001 |
Nile red (also known as Nile blue oxazone) is a lipophilic stain. It has environment-sensitive fluorescence. Nile red is intensely fluorescent in a lipid-rich environment while it has minimal fluorescence in aqueous media. It is an excellent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytofluorometry. Nile red stains intracellular lipid droplets red. Better selectivity for cytoplasmic lipid droplets can be obtained when the cells are viewed for yellow-gold fluorescence (450-500 nm excitation; >528 nm emission) rather than red fluorescence (515-560 nm excitation; >590 nm emission). It is strongly fluorescent, but only in a hydrophobic environment.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Nile Red *CAS 7385-67-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 314.1 µL | 1.57 mL | 3.141 mL | 15.705 mL | 31.41 mL |
5 mM | 62.82 µL | 314.1 µL | 628.2 µL | 3.141 mL | 6.282 mL |
10 mM | 31.41 µL | 157.05 µL | 314.1 µL | 1.57 mL | 3.141 mL |
Molarity calculator
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Absorbance (nm) | 560 |
Extinction coefficient (cm -1 M -1) | 38000 |
Excitation (nm) | 559 |
Emission (nm) | 635 |
Quantum yield | 0.70001 |
Images
Figure 1. Glucose-induced accumulation of neutral lipids in MPCCs after 10 days of treatment. Phase contrast images of MPCCs in hypo- (A), normo- (B) and hyperglycemic (C) culture medium after 10 days of treatment. Nile red (neutral lipids) staining of MPCCs in hypo- (D), normo- (E) and hyperglycemic (F) culture medium after 10 days of treatment. Circles highlight hepatocyte island location and scale bar is 400 µm. Source: Long-term exposure to abnormal glucose levels alters drug metabolism pathways and insulin sensitivity in primary human hepatocytes by Davidson et al., Scientific Reports, June 2016.
Figure 2. D. komarekii were stained with Nile red. Arrowheads indicate the large unicell populations. (a, b) Control group: (a) lipids visualized by Nile red staining and (b) bright field. (c, d) Cultured under 500 µM IAA: (c) lipids visualized by Nile red staining and (d) bright field. The yellow fluorescence was from oil bodies and the red fluorescence was from chloroplasts. Bar: 20 μm. Source: Indole-3-acetic-acid-induced phenotypic plasticity in Desmodesmus algae by Chung et al., Scientific Reports, July 2018.
Citations
View all 11 citations: Citation Explorer
SPG15 protein deficits are at the crossroads between lysosomal abnormalities, altered lipid metabolism and synaptic dysfunction
Authors: Marrone, Lara and Marchi, Paolo M and Webster, Christopher P and Marroccella, Raffaele and Coldicott, Ian and Reynolds, Steven and Alves-Cruzeiro, Jo{\~a}o and Yang, Zih-Liang and Higginbottom, Adrian and Khundadze, Mukhran and others,
Journal: Human Molecular Genetics (2022): 2693--2710
Authors: Marrone, Lara and Marchi, Paolo M and Webster, Christopher P and Marroccella, Raffaele and Coldicott, Ian and Reynolds, Steven and Alves-Cruzeiro, Jo{\~a}o and Yang, Zih-Liang and Higginbottom, Adrian and Khundadze, Mukhran and others,
Journal: Human Molecular Genetics (2022): 2693--2710
Dairy Cattle Nutrition and its Effect on Production, Milk, and Human Health
Authors: Swanson, Katherine L
Journal: (2018)
Authors: Swanson, Katherine L
Journal: (2018)
Depot-specific characteristics of adipose tissue-derived stromal cells in thyroid-associated orbitopathy
Authors: Wong, Janice Siu Chong and Chu, Wai Kit and Li, Benjamin Fuk-Loi and Pang, Chi-Pui and Chong, Kelvin Kam-lung
Journal: British Journal of Ophthalmology (2018): bjophthalmol--2017
Authors: Wong, Janice Siu Chong and Chu, Wai Kit and Li, Benjamin Fuk-Loi and Pang, Chi-Pui and Chong, Kelvin Kam-lung
Journal: British Journal of Ophthalmology (2018): bjophthalmol--2017
CRISPR/Cas9--mediated Tspo gene mutations lead to reduced mitochondrial membrane potential and steroid formation in MA-10 mouse tumor Leydig cells
Authors: Fan, Jinjiang and Wang, Kevin and Zirkin, Barry and Papadopoulos, Vassilios
Journal: Endocrinology (2018): 1130--1146
Authors: Fan, Jinjiang and Wang, Kevin and Zirkin, Barry and Papadopoulos, Vassilios
Journal: Endocrinology (2018): 1130--1146
Indole-3-acetic-acid-induced phenotypic plasticity in Desmodesmus algae
Authors: Chung, Tan-Ya and Kuo, Chih-Yen and Lin, Wei-Jiun and Wang, Wei-Lung and Chou, Jui-Yu
Journal: Scientific reports (2018): 10270
Authors: Chung, Tan-Ya and Kuo, Chih-Yen and Lin, Wei-Jiun and Wang, Wei-Lung and Chou, Jui-Yu
Journal: Scientific reports (2018): 10270
CRISPR/CAS9-mediated Tspo gene mutations lead to reduced mitochondrial membrane potential and STEROID FORMation in MA-10 mouse tumor Leydig cells
Authors: Fan, Jinjiang and Wang, Kevin and Zirkin, Barry and Papadopoulos, Vassilios
Journal: Endocrinology (2017)
Authors: Fan, Jinjiang and Wang, Kevin and Zirkin, Barry and Papadopoulos, Vassilios
Journal: Endocrinology (2017)
Long-term exposure to abnormal glucose levels alters drug metabolism pathways and insulin sensitivity in primary human hepatocytes
Authors: Davidson, Matthew D and Ballinger, Kimberly R and Khetani, Salman R
Journal: Scientific Reports (2016)
Authors: Davidson, Matthew D and Ballinger, Kimberly R and Khetani, Salman R
Journal: Scientific Reports (2016)
Hormone and drug-mediated modulation of glucose metabolism in a microscale model of the human liver
Authors: Davidson, Matthew D and Lehrer, Michael and Khetani, Salman R
Journal: Tissue Engineering Part C: Methods (2015): 716--725
Authors: Davidson, Matthew D and Lehrer, Michael and Khetani, Salman R
Journal: Tissue Engineering Part C: Methods (2015): 716--725
Hepatocyte nuclear factor 4α and downstream secreted phospholipase A2 GXIIB regulate production of infectious hepatitis C virus
Authors: Li, Xinlei and Jiang, Hanfang and Qu, Linbing and Yao, Wenxia and Cai, Hua and Chen, Ling and Peng, Tao
Journal: Journal of virology (2014): 612--627
Authors: Li, Xinlei and Jiang, Hanfang and Qu, Linbing and Yao, Wenxia and Cai, Hua and Chen, Ling and Peng, Tao
Journal: Journal of virology (2014): 612--627
Modulation of host cell translation and metabolism by Kaposi's sarcoma-associated herpesvirus
Authors: Bhatt, Aadra
Journal: (2013)
Authors: Bhatt, Aadra
Journal: (2013)
References
View all 73 references: Citation Explorer
Molten globule formation in apomyoglobin monitored by the fluorescent probe Nile Red
Authors: Polverini E, Cugini G, Annoni F, Abbruzzetti S, Viappiani C, Gensch T.
Journal: Biochemistry (2006): 5111
Authors: Polverini E, Cugini G, Annoni F, Abbruzzetti S, Viappiani C, Gensch T.
Journal: Biochemistry (2006): 5111
A clinical flow cytometric biomarker strategy: validation of peripheral leukocyte phospholipidosis using Nile red
Authors: Halstead BW, Zwickl CM, Morgan RE, Monteith DK, Thomas CE, Bowers RK, Berridge BR.
Journal: J Appl Toxicol (2006): 169
Authors: Halstead BW, Zwickl CM, Morgan RE, Monteith DK, Thomas CE, Bowers RK, Berridge BR.
Journal: J Appl Toxicol (2006): 169
A broad-spectrum fluorescence-based peptide library for the rapid identification of protease substrates
Authors: Thomas DA, Francis P, Smith C, Ratcliffe S, Ede NJ, Kay C, Wayne G, Martin SL, Moore K, Amour A, Hooper NM.
Journal: Proteomics (2006): 2112
Authors: Thomas DA, Francis P, Smith C, Ratcliffe S, Ede NJ, Kay C, Wayne G, Martin SL, Moore K, Amour A, Hooper NM.
Journal: Proteomics (2006): 2112
Membrane localization and dynamics of Nile Red: Effect of cholesterol
Authors: Mukherjee S, Raghuraman H, Chattopadhyay A.
Journal: Biochim Biophys Acta. (2006)
Authors: Mukherjee S, Raghuraman H, Chattopadhyay A.
Journal: Biochim Biophys Acta. (2006)
West Nile virus antibody prevalence in red-winged blackbirds (Agelaius phoeniceus) from North Dakota, USA (2003-2004)
Authors: Sullivan H, Linz G, Clark L, Salman M.
Journal: Vector Borne Zoonotic Dis (2006): 305
Authors: Sullivan H, Linz G, Clark L, Salman M.
Journal: Vector Borne Zoonotic Dis (2006): 305
Cationic liposomes in mixed didodecyldimethylammonium bromide and dioctadecyldimethylammonium bromide aqueous dispersions studied by differential scanning calorimetry, nile red fluorescence, and turbidity
Authors: Feitosa E, Alves FR, Niemiec A, Real Oliveira ME, Castanheira EM, Baptista AL.
Journal: Langmuir (2006): 3579
Authors: Feitosa E, Alves FR, Niemiec A, Real Oliveira ME, Castanheira EM, Baptista AL.
Journal: Langmuir (2006): 3579
Spectroscopic characterization of poly(amidoamine) dendrimers as selective uptake devices: Phenol blue versus Nile red
Authors: Morgan EJ, Rippey JM, Tucker SA.
Journal: Appl Spectrosc (2006): 551
Authors: Morgan EJ, Rippey JM, Tucker SA.
Journal: Appl Spectrosc (2006): 551
West Nile virus epizootiology, central Red River Valley, North Dakota and Minnesota, 2002-2005
Authors: Bell JA, Brewer CM, Mickelson NJ, Garman GW, Vaughan JA.
Journal: Emerg Infect Dis (2006): 1245
Authors: Bell JA, Brewer CM, Mickelson NJ, Garman GW, Vaughan JA.
Journal: Emerg Infect Dis (2006): 1245
Nile Red-adsorbed gold nanoparticle matrixes for determining aminothiols through surface-assisted laser desorption/ionization mass spectrometry
Authors: Huang YF, Chang HT.
Journal: Anal Chem (2006): 1485
Authors: Huang YF, Chang HT.
Journal: Anal Chem (2006): 1485
Studies on the interaction of Nile red with horseradish peroxidase in solution
Authors: Hungerford G, Rei A, Ferreira MI.
Journal: Febs J (2005): 6161
Authors: Hungerford G, Rei A, Ferreira MI.
Journal: Febs J (2005): 6161