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Nile Red
CAS 7385-67-3
Nile red (also known as Nile blue oxazone) is a lipophilic stain. It has environment-sensitive fluorescence. Nile red is intensely fluorescent in a lipid-rich environment while it has minimal fluorescence in aqueous media. It is an excellent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytofluorometry. Nile red stains intracellular lipid droplets red. Better selectivity for cytoplasmic lipid droplets can be obtained when the cells are viewed for yellow-gold fluorescence (450-500 nm excitation; >528 nm emission) rather than red fluorescence (515-560 nm excitation; >590 nm emission). It is strongly fluorescent, but only in a hydrophobic environment.
Glucose-induced accumulation of neutral lipids in MPCCs after 10 days of treatment. Phase contrast images of MPCCs in hypo- (A), normo- (B) and hyperglycemic (C) culture medium after 10 days of treatment. Nile red (neutral lipids) staining of MPCCs in hypo- (D), normo- (E) and hyperglycemic (F) culture medium after 10 days of treatment. Circles highlight hepatocyte island location and scale bar is 400 &micro;m. Source: <strong>Long-term exposure to abnormal glucose levels alters drug metabolism pathways and insulin sensitivity in primary human hepatocytes </strong>by Davidson et al., <em>Scientific Reports</em>, June 2016.
Glucose-induced accumulation of neutral lipids in MPCCs after 10 days of treatment. Phase contrast images of MPCCs in hypo- (A), normo- (B) and hyperglycemic (C) culture medium after 10 days of treatment. Nile red (neutral lipids) staining of MPCCs in hypo- (D), normo- (E) and hyperglycemic (F) culture medium after 10 days of treatment. Circles highlight hepatocyte island location and scale bar is 400 &micro;m. Source: <strong>Long-term exposure to abnormal glucose levels alters drug metabolism pathways and insulin sensitivity in primary human hepatocytes </strong>by Davidson et al., <em>Scientific Reports</em>, June 2016.
CatalogSize
Price
Quantity
2219025 mg
Price
 
Physical properties

Molecular weight318.37
SolventDMSO
Spectral properties

Absorbance (nm)560
Extinction coefficient (cm -1 M -1)
38000
Excitation (nm)559
Emission (nm)635
Quantum yield
0.7000
1
Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
CAS7385-67-3
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Page updated on October 13, 2025