Nile Red *CAS 7385-67-3*

Nile Red

CAS 7385-67-3

Nile red (also known as Nile blue oxazone) is a lipophilic stain. It has environment-sensitive fluorescence. Nile red is intensely fluorescent in a lipid-rich environment while it has minimal fluorescence in aqueous media. It is an excellent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytofluorometry. Nile red stains intracellular lipid droplets red. Better selectivity for cytoplasmic lipid droplets can be obtained when the cells are viewed for yellow-gold fluorescence (450-500 nm excitation; >528 nm emission) rather than red fluorescence (515-560 nm excitation; >590 nm emission). It is strongly fluorescent, but only in a hydrophobic environment.

Glucose-induced accumulation of neutral lipids in MPCCs after 10 days of treatment. Phase contrast images of MPCCs in hypo- (A), normo- (B) and hyperglycemic (C) culture medium after 10 days of treatment. Nile red (neutral lipids) staining of MPCCs in hypo- (D), normo- (E) and hyperglycemic (F) culture medium after 10 days of treatment. Circles highlight hepatocyte island location and scale bar is 400 µm. Source: <strong>Long-term exposure to abnormal glucose levels alters drug metabolism pathways and insulin sensitivity in primary human hepatocytes </strong>by Davidson et al., <em>Scientific Reports</em>, June 2016.

Catalog	Size	Price	Quantity
22190	25 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Nile Red *CAS 7385-67-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	314.1 µL	1.57 mL	3.141 mL	15.705 mL	31.41 mL
5 mM	62.82 µL	314.1 µL	628.2 µL	3.141 mL	6.282 mL
10 mM	31.41 µL	157.05 µL	314.1 µL	1.57 mL	3.141 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	318.37 g/mol	=	mL	x	mM	=	mmol

Citations

View all 13 citations: Citation Explorer

Bovine milk consumption affects the transcriptome of porcine adipose stem cells: Do exosomes play any role?

Authors:

Swanson, Katherine and Bell, Jimmy and Hendrix, David and Jiang, Duo and Kutzler, Michelle and Batty, Brandon and Hanlon, Melanie and Bionaz, Massimo

Journal:

PloS one (2024): e0302702

Characterizing $\alpha$-Synuclein Polymorphs Through AFM, SAA, Spectrophotometry and Digestion Assay

Authors:

Chen, W

SPG15 protein deficits are at the crossroads between lysosomal abnormalities, altered lipid metabolism and synaptic dysfunction

Authors:

Marrone, Lara and Marchi, Paolo M and Webster, Christopher P and Marroccella, Raffaele and Coldicott, Ian and Reynolds, Steven and Alves-Cruzeiro, Jo{\~a}o and Yang, Zih-Liang and Higginbottom, Adrian and Khundadze, Mukhran and others,

Journal:

Human Molecular Genetics (2022): 2693--2710

Dairy Cattle Nutrition and its Effect on Production, Milk, and Human Health

Authors:

Swanson, Katherine L

CRISPR/Cas9--mediated Tspo gene mutations lead to reduced mitochondrial membrane potential and steroid formation in MA-10 mouse tumor Leydig cells

Authors:

Fan, Jinjiang and Wang, Kevin and Zirkin, Barry and Papadopoulos, Vassilios

Journal:

Endocrinology (2018): 1130--1146

References

View all 73 references: Citation Explorer

Nile Red-adsorbed gold nanoparticle matrixes for determining aminothiols through surface-assisted laser desorption/ionization mass spectrometry

Authors:

Huang YF, Chang HT.

Journal:

Anal Chem (2006): 1485

West Nile virus epizootiology, central Red River Valley, North Dakota and Minnesota, 2002-2005

Authors:

Bell JA, Brewer CM, Mickelson NJ, Garman GW, Vaughan JA.

Journal:

Emerg Infect Dis (2006): 1245

Spectroscopic characterization of poly(amidoamine) dendrimers as selective uptake devices: Phenol blue versus Nile red

Authors:

Morgan EJ, Rippey JM, Tucker SA.

Journal:

Appl Spectrosc (2006): 551

Cationic liposomes in mixed didodecyldimethylammonium bromide and dioctadecyldimethylammonium bromide aqueous dispersions studied by differential scanning calorimetry, nile red fluorescence, and turbidity

Authors:

Feitosa E, Alves FR, Niemiec A, Real Oliveira ME, Castanheira EM, Baptista AL.

Journal:

Langmuir (2006): 3579

West Nile virus antibody prevalence in red-winged blackbirds (Agelaius phoeniceus) from North Dakota, USA (2003-2004)

Authors:

Sullivan H, Linz G, Clark L, Salman M.

Journal:

Vector Borne Zoonotic Dis (2006): 305

Physical properties

Molecular weight	318.37
Solvent	DMSO

Spectral properties

Absorbance (nm)	560
Extinction coefficient (cm ^-1 M ^-1)	38000
Excitation (nm)	559
Emission (nm)	635
Quantum yield	0.7000 ¹

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	7385-67-3

Documents

Protocol
Safety Data Sheet
Certificate of Origin
Certificate of Analysis

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Page updated on October 20, 2025