Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak.  Learn more >>

Nile Red *CAS 7385-67-3*

Glucose-induced accumulation of neutral lipids in MPCCs after 10 days of treatment. Phase contrast images of MPCCs in hypo- (A), normo- (B) and hyperglycemic (C) culture medium after 10 days of treatment. Nile red (neutral lipids) staining of MPCCs in hypo- (D), normo- (E) and hyperglycemic (F) culture medium after 10 days of treatment. Circles highlight hepatocyte island location and scale bar is 400 µm. Source: <strong>Long-term exposure to abnormal glucose levels alters drug metabolism pathways and insulin sensitivity in primary human hepatocytes </strong>by Davidson et al., <em>Scientific Reports</em>, June 2016.
Glucose-induced accumulation of neutral lipids in MPCCs after 10 days of treatment. Phase contrast images of MPCCs in hypo- (A), normo- (B) and hyperglycemic (C) culture medium after 10 days of treatment. Nile red (neutral lipids) staining of MPCCs in hypo- (D), normo- (E) and hyperglycemic (F) culture medium after 10 days of treatment. Circles highlight hepatocyte island location and scale bar is 400 µm. Source: <strong>Long-term exposure to abnormal glucose levels alters drug metabolism pathways and insulin sensitivity in primary human hepatocytes </strong>by Davidson et al., <em>Scientific Reports</em>, June 2016.
<em>D. komarekii </em>were stained with Nile red. Arrowheads indicate the large unicell populations. (a, b) Control group: (a) lipids visualized by Nile red staining and (b) bright field. (c, d) Cultured under 500 µM IAA: (c) lipids visualized by Nile red staining and (d) bright field. The yellow fluorescence was from oil bodies and the red fluorescence was from chloroplasts. Bar: 20 μm. Source: <strong>Indole-3-acetic-acid-induced phenotypic plasticity in <em>Desmodesmus</em> algae</strong> by Chung et al., <em>Scientific Reports</em>, July 2018.
Chemical structure for Nile Red *CAS 7385-67-3*
Ordering information
Price ()
Catalog Number22190
Unit Size
Find Distributor
Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight318.37
SolventDMSO
Spectral properties
Absorbance (nm)560
Extinction coefficient (cm -1 M -1)38000
Excitation (nm)559
Emission (nm)635
Quantum yield0.70001
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


CAS
7385-67-3
Molecular weight
318.37
Absorbance (nm)
560
Extinction coefficient (cm -1 M -1)
38000
Excitation (nm)
559
Emission (nm)
635
Quantum yield
0.70001
Nile red (also known as Nile blue oxazone) is a lipophilic stain. It has environment-sensitive fluorescence. Nile red is intensely fluorescent in a lipid-rich environment while it has minimal fluorescence in aqueous media. It is an excellent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytofluorometry. Nile red stains intracellular lipid droplets red. Better selectivity for cytoplasmic lipid droplets can be obtained when the cells are viewed for yellow-gold fluorescence (450-500 nm excitation; >528 nm emission) rather than red fluorescence (515-560 nm excitation; >590 nm emission). It is strongly fluorescent, but only in a hydrophobic environment.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Nile Red *CAS 7385-67-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM314.1 µL1.57 mL3.141 mL15.705 mL31.41 mL
5 mM62.82 µL314.1 µL628.2 µL3.141 mL6.282 mL
10 mM31.41 µL157.05 µL314.1 µL1.57 mL3.141 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Absorbance (nm)560
Extinction coefficient (cm -1 M -1)38000
Excitation (nm)559
Emission (nm)635
Quantum yield0.70001

Product family


NameExcitation (nm)Emission (nm)
Amplite® Red571584
LysoBrite™ Red576596
Congo Red *UltraPure grade* *CAS 573-58-0*--

Citations


View all 11 citations: Citation Explorer
SPG15 protein deficits are at the crossroads between lysosomal abnormalities, altered lipid metabolism and synaptic dysfunction
Authors: Marrone, Lara and Marchi, Paolo M and Webster, Christopher P and Marroccella, Raffaele and Coldicott, Ian and Reynolds, Steven and Alves-Cruzeiro, Jo{\~a}o and Yang, Zih-Liang and Higginbottom, Adrian and Khundadze, Mukhran and others,
Journal: Human Molecular Genetics (2022): 2693--2710
Dairy Cattle Nutrition and its Effect on Production, Milk, and Human Health
Authors: Swanson, Katherine L
Journal: (2018)
Depot-specific characteristics of adipose tissue-derived stromal cells in thyroid-associated orbitopathy
Authors: Wong, Janice Siu Chong and Chu, Wai Kit and Li, Benjamin Fuk-Loi and Pang, Chi-Pui and Chong, Kelvin Kam-lung
Journal: British Journal of Ophthalmology (2018): bjophthalmol--2017
CRISPR/Cas9--mediated Tspo gene mutations lead to reduced mitochondrial membrane potential and steroid formation in MA-10 mouse tumor Leydig cells
Authors: Fan, Jinjiang and Wang, Kevin and Zirkin, Barry and Papadopoulos, Vassilios
Journal: Endocrinology (2018): 1130--1146
Indole-3-acetic-acid-induced phenotypic plasticity in Desmodesmus algae
Authors: Chung, Tan-Ya and Kuo, Chih-Yen and Lin, Wei-Jiun and Wang, Wei-Lung and Chou, Jui-Yu
Journal: Scientific reports (2018): 10270
CRISPR/CAS9-mediated Tspo gene mutations lead to reduced mitochondrial membrane potential and STEROID FORMation in MA-10 mouse tumor Leydig cells
Authors: Fan, Jinjiang and Wang, Kevin and Zirkin, Barry and Papadopoulos, Vassilios
Journal: Endocrinology (2017)
Long-term exposure to abnormal glucose levels alters drug metabolism pathways and insulin sensitivity in primary human hepatocytes
Authors: Davidson, Matthew D and Ballinger, Kimberly R and Khetani, Salman R
Journal: Scientific Reports (2016)
Hormone and drug-mediated modulation of glucose metabolism in a microscale model of the human liver
Authors: Davidson, Matthew D and Lehrer, Michael and Khetani, Salman R
Journal: Tissue Engineering Part C: Methods (2015): 716--725
Hepatocyte nuclear factor 4α and downstream secreted phospholipase A2 GXIIB regulate production of infectious hepatitis C virus
Authors: Li, Xinlei and Jiang, Hanfang and Qu, Linbing and Yao, Wenxia and Cai, Hua and Chen, Ling and Peng, Tao
Journal: Journal of virology (2014): 612--627

References


View all 73 references: Citation Explorer
Molten globule formation in apomyoglobin monitored by the fluorescent probe Nile Red
Authors: Polverini E, Cugini G, Annoni F, Abbruzzetti S, Viappiani C, Gensch T.
Journal: Biochemistry (2006): 5111
A clinical flow cytometric biomarker strategy: validation of peripheral leukocyte phospholipidosis using Nile red
Authors: Halstead BW, Zwickl CM, Morgan RE, Monteith DK, Thomas CE, Bowers RK, Berridge BR.
Journal: J Appl Toxicol (2006): 169
A broad-spectrum fluorescence-based peptide library for the rapid identification of protease substrates
Authors: Thomas DA, Francis P, Smith C, Ratcliffe S, Ede NJ, Kay C, Wayne G, Martin SL, Moore K, Amour A, Hooper NM.
Journal: Proteomics (2006): 2112
Membrane localization and dynamics of Nile Red: Effect of cholesterol
Authors: Mukherjee S, Raghuraman H, Chattopadhyay A.
Journal: Biochim Biophys Acta. (2006)
West Nile virus antibody prevalence in red-winged blackbirds (Agelaius phoeniceus) from North Dakota, USA (2003-2004)
Authors: Sullivan H, Linz G, Clark L, Salman M.
Journal: Vector Borne Zoonotic Dis (2006): 305
Cationic liposomes in mixed didodecyldimethylammonium bromide and dioctadecyldimethylammonium bromide aqueous dispersions studied by differential scanning calorimetry, nile red fluorescence, and turbidity
Authors: Feitosa E, Alves FR, Niemiec A, Real Oliveira ME, Castanheira EM, Baptista AL.
Journal: Langmuir (2006): 3579
Spectroscopic characterization of poly(amidoamine) dendrimers as selective uptake devices: Phenol blue versus Nile red
Authors: Morgan EJ, Rippey JM, Tucker SA.
Journal: Appl Spectrosc (2006): 551
West Nile virus epizootiology, central Red River Valley, North Dakota and Minnesota, 2002-2005
Authors: Bell JA, Brewer CM, Mickelson NJ, Garman GW, Vaughan JA.
Journal: Emerg Infect Dis (2006): 1245
Nile Red-adsorbed gold nanoparticle matrixes for determining aminothiols through surface-assisted laser desorption/ionization mass spectrometry
Authors: Huang YF, Chang HT.
Journal: Anal Chem (2006): 1485
Studies on the interaction of Nile red with horseradish peroxidase in solution
Authors: Hungerford G, Rei A, Ferreira MI.
Journal: Febs J (2005): 6161