Phalloidin Conjugates | AAT Bioquest

Phalloidin-iFluor® 555 Conjugate

Phalloidin-iFluor® 555 Conjugate is an orange-fluorescent probe designed for selective detection and visualization of filamentous actin (F-actin) in fixed and permeabilized cells and tissues.

Binding Specificity: Selectively binds F-actin with high affinity.
Spectral Properties: Excitation/emission maxima of 557/570 nm.
Instrument Compatibility: Compatible with TRITC fluorescence microscope filter sets.
Applications: Suitable for actin cytoskeleton staining and multicolor fluorescence microscopy.

Multiplex fluorescence imaging of CPA47 cells stained with Phalloidin-iFluor® 555 Conjugate, MitoLite™ NIR FX690, and Nuclear Violet™ LCS1. Cells were fixed with 4% formaldehyde, permeabilized with 0.2% Triton X-100, and stained with Phalloidin-iFluor® 555 Conjugate (Cat. #23119) to visualize F-actin (red), MitoLite™ NIR FX690 (Cat. #22690) to label mitochondria (green), and Nuclear Violet™ LCS1 (Cat. #17543) to stain nuclei (blue). Images were acquired using Cy3-TRITC, Cy5, and DAPI filter sets and merged to generate the overlay image.

Protocol

SDS

Catalog	Size	Price	Quantity
23119	300 Tests	Price

Overview

This orange fluorescent phalloidin conjugate (equivalent to Alexa Fluor® 555-labeled phalloidin) selectively binds to F-actins. Used at nanomolar concentrations, phalloidin derivatives are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. Phalloidin binds to actin filaments much more tightly than to actin monomers, leading to a decrease in the rate constant for the dissociation of actin subunits from filament ends, essentially stabilizing actin filaments through the prevention of filament depolymerization. Moreover, phalloidin is found to inhibit the ATP hydrolysis activity of F-actin. Phalloidin functions differently at various concentrations in cells. When introduced into the cytoplasm at low concentrations, phalloidin recruits the less polymerized forms of cytoplasmic actin as well as filamin into stable "islands" of aggregated actin polymers, yet it does not interfere with stress fibers, i.e. thick bundles of microfilaments. The property of phalloidin is a useful tool for investigating the distribution of F-actin in cells by labeling phalloidin with fluorescent analogs and using them to stain actin filaments for light microscopy. Fluorescent derivatives of phalloidin have turned out to be enormously useful in localizing actin filaments in living or fixed cells as well as for visualizing individual actin filaments in vitro. Fluorescent phalloidin derivatives have been used as an important tool in the study of actin networks at high resolution. AAT Bioquest offers a variety of fluorescent phalloidin derivatives with different colors for multicolor imaging applications.

Citations

View all 132 citations: Citation Explorer

A decline in skeletal muscle NOX4 abrogates exercise-induced adaptive homeostasis and exacerbates biological aging

Authors:

Xirouchaki, Chrysovalantou E and Garc{\'\i}a-Dom{\'\i}nguez, Esther and Coughlan, Eamon and McGrath, Meagan J and Giri, Saveen and Liang, Shuwei and Wiede, Florian and Bigot, Anne and Sadoshima, Junichi and Gomez-Cabrera, Maria C and others,

Journal:

Science Advances (2026): eadz1953

Imaging and safety profiling of inhaled siRNA RyR2 in human respiratory models

Authors:

Bettinsoli, Valeria and Erlacher, Magdalena and Galbiati, Valentina and Marinovich, Marina and Corsini, Emanuela and Wilflingseder, Doris

Journal:

Archives of Toxicology (2026): 1--9

PTBP1 supports mouse spermatogenesis by facilitating cytoskeletal organization through the mTORC2--PKC$\alpha$ pathway in Sertoli cells

Authors:

Ozawa, Manabu and Taguchi, Jumpei and Mori, Hideto and Kikuchi, Mio and Muratani, Masafumi and Yamamoto, Takuya and Yamada, Yasuhiro and Yoshida, Nobuaki and Ikawa, Masahito

Journal:

Communications Biology (2026)

Decoding the Role of Caveolin-1 in Morphological Diversity and Self-Renewal of Breast Cancer Cells

Authors:

Li, Shun and Duan, Hongyun and Yang, Lu and Jiang, Lingyi and Bian, Haocheng and Jiang, Yuqin and Zhang, Yixi and Yan, Wei and Yang, Qin and Li, Tingting and others,

Journal:

Cell Proliferation (2025): e70137

L-Asparagine is the essential factor for the susceptibility of Chinese pigs to Mycoplasma hyopneumoniae

Authors:

Zhao, Long and Hao, Fei and Xiong, Qiyan and Wei, Yanna and Zhang, Lei and Chen, Rong and Yu, Yanfei and Feng, Zhixin and Xie, Xing

Journal:

Food Production, Processing and Nutrition (2025): 17

References

View all 127 references: Citation Explorer

Improved penile histology by phalloidin stain: circular and longitudinal cavernous smooth muscles, dual-endothelium arteries, and erectile dysfunction-associated changes

Authors:

Lin G, Qiu X, F and el TM, Albersen M, Wang Z, Lue TF, Lin CS.

Journal:

Urology (2011): 970 e1

Phalloidin perturbs the interaction of human non-muscle myosin isoforms 2A and 2C1 with F-actin

Authors:

Diensthuber RP, Muller M, Heissler SM, Taft MH, Chizhov I, Manstein DJ.

Journal:

FEBS Lett (2011): 767

pH-(low)-insertion-peptide (pHLIP) translocation of membrane impermeable phalloidin toxin inhibits cancer cell proliferation

Authors:

An M, Wijesinghe D, Andreev OA, Reshetnyak YK, Engelman DM.

Journal:

Proc Natl Acad Sci U S A (2010): 20246

Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging

Authors:

Chazotte B., undefined

Journal:

Cold Spring Harb Protoc (2010): pdb prot4947

Protective effect of bile acid derivatives in phalloidin-induced rat liver toxicity

Authors:

Herraez E, Macias RI, Vazquez-Tato J, Hierro C, Monte MJ, Marin JJ.

Journal:

Toxicol Appl Pharmacol (2009): 21

Physical properties

Molecular weight	~1300
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.23
Correction factor (280 nm)	0.14
Extinction coefficient (cm ^-1 M ^-1)	100000 ¹
Excitation (nm)	557
Emission (nm)	570
Quantum yield	0.64 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 2, 2026