Name: 3'-(6-Fluorescein) CPG *1000 Å*
Brand: AAT Bioquest
SKU: 6014
Price: 465 USD
Availability: InStock

3'-(6-Fluorescein) CPG

1000 Å

There are several ways of labeling an oligonucleotide with fluorescein. The choice of label is diversified further, depending on the spectral requirements. 5'-fluorescein-CE phosphoramidite, derived from the single isomer 6-carboxyfluorescein, 5'-hexachlorofluorescein-CE phosphoramidite (HEX) and 5'-tetrachlorofluorescein-CE phosphoramidite (TET) can all be used to efficiently label an oligonucleotide at the 5'-end. Labeling the 3'-end of an oligo with fluorescein can be achieved using 3'-Fluorescein CPGs. Standard cleavage and deprotection with ammonium hydroxide liberates the fluorescein-labeled oligo when using any of these supports.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
6014	1 g	Price

References

View all 13 references: Citation Explorer

Sequence-specific, self-reporting hairpin inversion probes

Authors:

Browne KA., undefined

Journal:

J Am Chem Soc (2005): 1989

Modified oligonucleotides containing lithocholic acid in their backbones: their enhanced cellular uptake and their mimicking of hairpin structures

Authors:

Kim SJ, Bang EK, Kwon HJ, Shim JS, Kim BH.

Journal:

Chembiochem (2004): 1517

Synthesis of HyBeacons and dual-labelled probes containing 2'-fluorescent groups for use in genetic analysis

Authors:

Dobson N, McDowell DG, French DJ, Brown LJ, Mellor JM, Brown T.

Journal:

Chem Commun (Camb) (2003): 1234

Reduced aggregation and improved specificity of G-rich oligodeoxyribonucleotides containing pyrazolo[3,4-d]pyrimidine guanine bases

Authors:

Kutyavin IV, Lokhov SG, Afonina IA, Dempcy R, Gall AA, Gorn VV, Lukhtanov E, Metcalf M, Mills A, Reed MW, S and ers S, Shishkina I, Vermeulen NM.

Journal:

Nucleic Acids Res (2002): 4952

Diels--Alder bioconjugation of diene-modified oligonucleotides

Authors:

Hill KW, Taunton-Rigby J, Carter JD, Kropp E, Vagle K, Pieken W, McGee DP, Husar GM, Leuck M, Anziano DJ, Sebesta DP.

Journal:

J Org Chem (2001): 5352

Physical properties

Solvent

N/A

Spectral properties

Absorbance (nm)	487
Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000 ¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900 ¹ , 0.95 ²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 9, 2025