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3-Cyano-7-hydroxycoumarin *CAS 19088-73-4*

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Physical properties
Molecular weight187.15
SolventDMSO
Spectral properties
Extinction coefficient (cm -1 M -1)43000
Excitation (nm)406
Emission (nm)450
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


See also: Coumarins
CAS
19088-73-4
Molecular weight
187.15
Extinction coefficient (cm -1 M -1)
43000
Excitation (nm)
406
Emission (nm)
450
3-Cyano-7-hydroxycoumarin is used as a calibration standard for 3-cyano-7-hydroxycoumarin-based enzyme substrates. It has pH-dependent fluorescence.

Platform


Fluorescence microplate reader

Excitation408 nm
Emission450 nm
Cutoff420 nm
Recommended plateSolid black

Example protocol


AT A GLANCE

Important

Store at -20 °C, desiccated and protected from light. Expiration date is one year from the date of receipt.

PREPARATION OF STOCK SOLUTION

Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.

1. 3-Cyano-7-hydroxycoumarin stock solution (1mM):
Add 5.343 µL DMSO to 1 mg 3-Cyano-7-hydroxycoumarin to make 1 mM stock solution.  Note: Add appropriate amount of DMSO to make appropriate concentration of stock solution.

PREPARATION OF WORKING SOLUTION

1. 3-Cyano-7-hydroxycoumarin working solution:
Working solution can be diluted in phosphate or Tris buffer, pH 7.4-7.5. Note: Make appropriate concentration of working solution as per needed.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 3-Cyano-7-hydroxycoumarin *CAS 19088-73-4* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM534.331 µL2.672 mL5.343 mL26.717 mL53.433 mL
5 mM106.866 µL534.331 µL1.069 mL5.343 mL10.687 mL
10 mM53.433 µL267.165 µL534.331 µL2.672 mL5.343 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)43000
Excitation (nm)406
Emission (nm)450

Images


References


View all 3 references: Citation Explorer
Characterization of asymmetric fluorogenic phosphonates as probes for developing organophosphorus hydrolases with broader stereoselectivity
Authors: Amitai G, Adani R, Limanovich O, Teitlboim S, Yishay S, Tveria L, Yacov G, Meshulam H, Raveh L.
Journal: Chem Biol Interact (2008): 249
Asymmetric fluorogenic organophosphates for the development of active organophosphate hydrolases with reversed stereoselectivity
Authors: Amitai G, Adani R, Yacov G, Yishay S, Teitlboim S, Tveria L, Limanovich O, Kushnir M, Meshulam H.
Journal: Toxicology (2007): 187
Rapid determination of enzyme activities of recombinant human cytochromes P450, human liver microsomes and hepatocytes
Authors: Ghosal A, Hapangama N, Yuan Y, Lu X, Horne D, Patrick JE, Zbaida S.
Journal: Biopharm Drug Dispos (2003): 375