3-Cyano-7-hydroxycoumarin CAS 19088-73-4

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Physical properties

Molecular weight	187.15
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	43000
Excitation (nm)	406
Emission (nm)	450

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

19088-73-4

Molecular weight

187.15

Extinction coefficient (cm ^-1 M ^-1)

43000

Excitation (nm)

406

Emission (nm)

450

3-Cyano-7-hydroxycoumarin is used as a calibration standard for 3-cyano-7-hydroxycoumarin-based enzyme substrates. It has pH-dependent fluorescence.

Platform

Fluorescence microplate reader

Excitation	408 nm
Emission	450 nm
Cutoff	420 nm
Recommended plate	Solid black

Example protocol

AT A GLANCE

Important

Store at -20 °C, desiccated and protected from light. Expiration date is one year from the date of receipt.

PREPARATION OF STOCK SOLUTION

Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.

1. 3-Cyano-7-hydroxycoumarin stock solution (1mM):
Add 5.343 µL DMSO to 1 mg 3-Cyano-7-hydroxycoumarin to make 1 mM stock solution. Note: Add appropriate amount of DMSO to make appropriate concentration of stock solution.

PREPARATION OF WORKING SOLUTION

1. 3-Cyano-7-hydroxycoumarin working solution:
Working solution can be diluted in phosphate or Tris buffer, pH 7.4-7.5. Note: Make appropriate concentration of working solution as per needed.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 3-Cyano-7-hydroxycoumarin *CAS 19088-73-4* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	534.331 µL	2.672 mL	5.343 mL	26.717 mL	53.433 mL
5 mM	106.866 µL	534.331 µL	1.069 mL	5.343 mL	10.687 mL
10 mM	53.433 µL	267.165 µL	534.331 µL	2.672 mL	5.343 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	43000
Excitation (nm)	406
Emission (nm)	450

Images

Figure 1. Chemical structure for 3-Cyano-7-hydroxycoumarin *CAS 19088-73-4*

References

View all 3 references: Citation Explorer

Characterization of asymmetric fluorogenic phosphonates as probes for developing organophosphorus hydrolases with broader stereoselectivity
Authors: Amitai G, Adani R, Limanovich O, Teitlboim S, Yishay S, Tveria L, Yacov G, Meshulam H, Raveh L.
Journal: Chem Biol Interact (2008): 249

Asymmetric fluorogenic organophosphates for the development of active organophosphate hydrolases with reversed stereoselectivity
Authors: Amitai G, Adani R, Yacov G, Yishay S, Teitlboim S, Tveria L, Limanovich O, Kushnir M, Meshulam H.
Journal: Toxicology (2007): 187

Rapid determination of enzyme activities of recombinant human cytochromes P450, human liver microsomes and hepatocytes
Authors: Ghosal A, Hapangama N, Yuan Y, Lu X, Horne D, Patrick JE, Zbaida S.
Journal: Biopharm Drug Dispos (2003): 375