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3-Cyano-7-hydroxycoumarin *CAS 19088-73-4*
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Application notes
3-Cyano-7-hydroxycoumarin is used as a calibration standard for 3-cyano-7-hydroxycoumarin-based enzyme substrates. It has pH-dependent fluorescence.
Example protocol
AT A GLANCE
Important
Store at -20 °C, desiccated and protected from light. Expiration date is one year from the date of receipt.
PREPARATION OF STOCK SOLUTION
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.
1. 3-Cyano-7-hydroxycoumarin stock solution (1mM):
Add 5.343 µL DMSO to 1 mg 3-Cyano-7-hydroxycoumarin to make 1 mM stock solution. Note: Add appropriate amount of DMSO to make appropriate concentration of stock solution.
PREPARATION OF WORKING SOLUTION
1. 3-Cyano-7-hydroxycoumarin working solution:
Working solution can be diluted in phosphate or Tris buffer, pH 7.4-7.5. Note: Make appropriate concentration of working solution as per needed.
Spectrum
References
View all 3 references: Citation Explorer
Characterization of asymmetric fluorogenic phosphonates as probes for developing organophosphorus hydrolases with broader stereoselectivity
Authors: Amitai G, Adani R, Limanovich O, Teitlboim S, Yishay S, Tveria L, Yacov G, Meshulam H, Raveh L.
Journal: Chem Biol Interact (2008): 249
Authors: Amitai G, Adani R, Limanovich O, Teitlboim S, Yishay S, Tveria L, Yacov G, Meshulam H, Raveh L.
Journal: Chem Biol Interact (2008): 249
Asymmetric fluorogenic organophosphates for the development of active organophosphate hydrolases with reversed stereoselectivity
Authors: Amitai G, Adani R, Yacov G, Yishay S, Teitlboim S, Tveria L, Limanovich O, Kushnir M, Meshulam H.
Journal: Toxicology (2007): 187
Authors: Amitai G, Adani R, Yacov G, Yishay S, Teitlboim S, Tveria L, Limanovich O, Kushnir M, Meshulam H.
Journal: Toxicology (2007): 187
Rapid determination of enzyme activities of recombinant human cytochromes P450, human liver microsomes and hepatocytes
Authors: Ghosal A, Hapangama N, Yuan Y, Lu X, Horne D, Patrick JE, Zbaida S.
Journal: Biopharm Drug Dispos (2003): 375
Authors: Ghosal A, Hapangama N, Yuan Y, Lu X, Horne D, Patrick JE, Zbaida S.
Journal: Biopharm Drug Dispos (2003): 375