3-Maleimidopropionic acid N-hydroxysuccinimide ester *CAS 55750-62-4*

3-Maleimidopropionic acid N-hydroxysuccinimide ester

CAS 55750-62-4

3-Maleimidopropionic acid N-hydroxysuccinimide ester is a non-cleavable and membrane permeable crosslinker. It contains an amine-reactive succinimidyl ester (SE) and a sulfhydryl-reactive maleimide group. NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. The SE group of 3-maleimidopropionic acid N-hydroxysuccinimide ester is reacted with lysine residues on the carrier protein, converting them to reactive maleimides. 3-Maleimidopropionic acid N-hydroxysuccinimide ester is a reagent that is widely used for generating stable maleimide-activated carrier proteins that can spontaneously react with sulfhydryls. Alternatively these relatively stable maleimide-activated intermediates may be lyophilized and stored for later conjugation to a hapten.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
4507	100 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 3-Maleimidopropionic acid N-hydroxysuccinimide ester *CAS 55750-62-4* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	375.643 µL	1.878 mL	3.756 mL	18.782 mL	37.564 mL
5 mM	75.129 µL	375.643 µL	751.287 µL	3.756 mL	7.513 mL
10 mM	37.564 µL	187.822 µL	375.643 µL	1.878 mL	3.756 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	266.21 g/mol	=	mL	x	mM	=	mmol

References

View all 25 references: Citation Explorer

Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe

Authors:

Andreoni A, Bondani M, Nardo L.

Journal:

Photochem Photobiol Sci (2009): 1202

Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer

Authors:

Ogawa M, Kosaka N, Choyke PL, Kobayashi H.

Journal:

Bioconjug Chem (2009): 147

The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates

Authors:

Kim GI, Kim KW, Oh MK, Sung YM.

Journal:

Nanotechnology (2009): 175503

Development of a cell-based hepatitis C virus infection fluorescent resonance energy transfer assay for high-throughput antiviral compound screening

Authors:

Yu X, Sainz B, Jr., Uprichard SL.

Journal:

Antimicrob Agents Chemother (2009): 4311

An improved cell-penetrating, caspase-activatable, near-infrared fluorescent peptide for apoptosis imaging

Authors:

Maxwell D, Chang Q, Zhang X, Barnett EM, Piwnica-Worms D.

Journal:

Bioconjug Chem (2009): 702

Physical properties

Molecular weight	266.21
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C)
UNSPSC	12352200
CAS	55750-62-4

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