3-Maleimidopropionic acid N-hydroxysuccinimide ester *CAS 55750-62-4*
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Physical properties
Molecular weight | 266.21 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C) |
UNSPSC | 12352200 |
Overview | SDSProtocol |
CAS 55750-62-4 | Molecular weight 266.21 |
3-Maleimidopropionic acid N-hydroxysuccinimide ester is a non-cleavable and membrane permeable crosslinker. It contains an amine-reactive succinimidyl ester (SE) and a sulfhydryl-reactive maleimide group. NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. The SE group of 3-maleimidopropionic acid N-hydroxysuccinimide ester is reacted with lysine residues on the carrier protein, converting them to reactive maleimides. 3-Maleimidopropionic acid N-hydroxysuccinimide ester is a reagent that is widely used for generating stable maleimide-activated carrier proteins that can spontaneously react with sulfhydryls. Alternatively these relatively stable maleimide-activated intermediates may be lyophilized and stored for later conjugation to a hapten.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 3-Maleimidopropionic acid N-hydroxysuccinimide ester *CAS 55750-62-4* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 375.643 µL | 1.878 mL | 3.756 mL | 18.782 mL | 37.564 mL |
5 mM | 75.129 µL | 375.643 µL | 751.287 µL | 3.756 mL | 7.513 mL |
10 mM | 37.564 µL | 187.822 µL | 375.643 µL | 1.878 mL | 3.756 mL |
Molarity calculator
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Citations
View all 1 citations: Citation Explorer
N-glycan targeted gene delivery to the dendritic cell SIGN receptor
Authors: Anderson, Kevin and Fern, undefined and ez, Christian and Rice, Kevin G
Journal: Bioconjugate chemistry (2010): 1479--1485
Authors: Anderson, Kevin and Fern, undefined and ez, Christian and Rice, Kevin G
Journal: Bioconjugate chemistry (2010): 1479--1485
References
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