3'-O-Azidomethyl-dCTP is one of the four essential dNTPs (A, C, G, T) that can be used for the DNA sequencing by synthesis (SBS). DNA sequencing is a fundamental tool in biological and medical research (especially for personalized medicine). Various DNA sequencing methods have been developed. Currently, the dominant method is sequencing by synthesis (SBS), an approach that determines DNA sequences during the polymerase reaction. The most widely used high-throughput SBS technology uses cleavable fluorescent reversible nucleotide terminator sequencing chemistry. The fluorescence based SBS suffers limited read length since the current cleavable fluorescent dNTPs used in SBS leave a large fluorescence tag on the base of the growing DNA strand. Conceptually, the much smaller azidomethyl group on the dNTPs may be used as a tag for sequencing. Due to the small size of the azido label, the 3′-O-azidomethyl-dNTPs are efficient substrates for the DNA polymerase. In the SBS cycles, the natural nucleotides are restored after each incorporation and cleavage, producing a growing DNA strand that bears no modifications and will not impede further polymerase reactions.