Calculator
5-dR110, succinimidyl ester
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 540.31 |
| Solvent | DMSO |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
| Overview |  SDSProtocol |
See also: Amine Reactive Dyes and Probes for Conjugation, Rhodamines and Rhodamine Derivatives, Chromatin Immunoprecipitation (ChIP), Fluoresceins, Gene Expression Analysis & Genotyping, Genome Editing & CRISPR, Next Generation Sequencing (NGS), Nucleic Acid Building Blocks
Molecular weight 540.31 |
Compared to fluorescein labeling reagents such as FITC and FAM, 5-dR110 [5-carboxy-4,7-dichlororhodamine 110] reagents give more photostable and pH-independent bioconjugates that have the absorption maximum around the preferred 488 nm excitation wavelength. They are photostable alternative reagents superior to FITC and FAM. 5-carboxy-4,7-dichlororhodamine (dR110) is one of the four critical 4,7-dichloro-rhodamine dyes used as the BigDye terminators for DNA sequencing in combination with d6G, dTMR and dROX. ABI PRISM® BigDye™ Terminator Cycle Sequencing is the classic method for DNA sequencing. 5-dR110, succinimidyl ester is the most convenient building block for preparing the dR110 color of BigDye Sanger sequencing fluorophore set.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 5-dR110, succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 185.079 µL | 925.395 µL | 1.851 mL | 9.254 mL | 18.508 mL |
| 5 mM | 37.016 µL | 185.079 µL | 370.158 µL | 1.851 mL | 3.702 mL |
| 10 mM | 18.508 µL | 92.539 µL | 185.079 µL | 925.395 µL | 1.851 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Images
Figure 1. Chemical structure for 5-dR110, succinimidyl ester.