Screen Quest™ Fluorimetric ELISA cAMP Assay Kit

Screen Quest™ Fluorescence ELISA cAMP Assay Kit provides an optimized assay method for monitoring the activation of adenylyl cyclase in G-protein coupled receptor systems. The assay is based on the competition for a fixed number of antibody binding sites between HRP-labeled cAMP and non-labeled cAMP. HRP-cAMP is displaced from the HRP-cAMP/anti-cAMP antibody complex by unlabeled free cAMP. In the absence of cAMP, HRP-cAMP conjugate is bound to anti-cAMP antibody exclusively. However, the unlabeled free cAMP in the test sample competes for anti-cAMP antibody with the HRP-cAMP antibody conjugate, therefore inhibits the binding of HRP-cAMP to anti-cAMP antibody. Our Screen Quest™ Fluorometric cAMP Assay Kit provides a sensitive method for detecting adenylate cyclase activity. Compared to other commercial ELISA cAMP assay kits, this cAMP assay kit only requires a single wash step to remove unbound material prior to the development step. It also eliminates the tedious acetylation step. The kit uses Amplite® Red as a fluorogenic HRP substrate to quantify the HRP activity. The fluorescent product formed is proportional to the activity of HRP-cAMP conjugate.

Protocol

Catalog	Size	Price	Quantity
36373	1 plate	Price
36374	10 plates	Price

Components

Catalog 36373 (1 plate)

Component A: cAMP Standard	1 vial (33 µg)
Component B: Assay Buffer	1 bottle (20 mL)
Component C: HRP-cAMP Conjugate	1 vial
Component D: 10X Wash Solution	1 bottle (10 mL)
Component E: Cell Lysis Buffer	1 bottle (10 mL)
Component F: 3% H2O2	1 vial (50 µL)
Component G: Amplite® Red	1 vial
Component H: Anti-cAMP Ab Coated 96-Well Plate	1 plate
Component I: Substrate Buffer	1 bottle (10 mL)

Catalog 36374 (10 plates)

Component A: cAMP Standard	1 vial (33 µg)
Component B: Assay Buffer	1 bottle (100 mL)
Component C: HRP-cAMP Conjugate	5 vials
Component D: 10X Wash Solution	1 bottle (100 mL)
Component E: Cell Lysis Buffer	1 bottle (100 mL)
Component F: 3% H2O2	1 vial (250 µL)
Component G: Amplite® Red	1 vial
Component H: Anti-cAMP Ab Coated 96-Well Plate	10 plates
Component I: Substrate Buffer	1 bottle (100 mL)

Citations

View all 5 citations: Citation Explorer

Propionate suppresses colitis-associated colorectal cancer in mice by Or51e2

Authors:

Kim, Ji-Sun and Jeong, Mi-Young and Yoon, Ye Eun and Kim, Yeonji and Lee, InRyeong and Choi, Jung-Won and Lee, Ha Lim and Shin, Min-Jeong and Hong, SungHoi and Chi, Sung-Gil and others,

Journal:

Biomedicine \& Pharmacotherapy (2025): 118268

OR2AT4, an Ectopic Olfactory Receptor, Suppresses Oxidative Stress-Induced Senescence in Human Keratinocytes

Authors:

Kim, Ji-Sun and Lee, Ha Lim and Jeong, Ji Hyun and Yoon, Ye Eun and Lee, In-Ryeong and Kim, Ji Min and Wu, Chunyan and Lee, Sung-Joon

Journal:

Antioxidants (2022): 2180

Albanin A, Derived from the Root Bark of Morus alba L., Depresses Glutamate Release in the Rat Cerebrocortical Nerve Terminals via Ca2+/Calmodulin/Adenylate Cyclase 1 Suppression

Authors:

Chang, Yi and Hung, Chi Feng and Ko, Horng Huey and Wang, Su Jane

Journal:

Journal of Medicinal Food (2021): 209--217

Activation of P2X7 and P2Y11 purinergic receptors inhibits migration and normalizes tumor-derived endothelial cells via cAMP signaling

Authors:

Avanzato, D and Genova, T and Pla, A Fiorio and Bernardini, M and Bianco, S and Bussolati, B and Mancardi, D and Giraudo, E and Maione, F and Cassoni, P and others, undefined

Journal:

Scientific Reports (2016)

The M2 muscarinic receptors are essential for signaling in the heart left ventricle during restraint stress in mice

Authors:

Tomankova, Hana and Valuskova, Paulina and Varejkova, Eva and Rotkova, Jana and Benes, Jan and Myslivecek, Jaromir

Journal:

Stress (2015)

References

View all 132 references: Citation Explorer

cAMP-Induced Histones H3 Dephosphorylation Is Independent of PKA and MAP Kinase Activations and Correlates With mTOR Inactivation

Authors:

Rodriguez P, Rojas J.

Journal:

J Cell Biochem (2016): 741

Changes in the Arabidopsis thaliana Proteome Implicate cAMP in Biotic and Abiotic Stress Responses and Changes in Energy Metabolism

Authors:

Alqurashi M, Gehring C, Marondedze C.

Journal:

Int J Mol Sci (2016): 852

Role of the cAMP Pathway in Glucose and Lipid Metabolism

Authors:

Ravnskjaer K, Madiraju A, Montminy M.

Journal:

Handb Exp Pharmacol (2016): 29

Odor-induced cAMP production in Drosophila melanogaster olfactory sensory neurons

Authors:

Miazzi F, Hansson BS, Wicher D.

Journal:

J Exp Biol (2016): 1798

A cardiac mitochondrial cAMP signaling pathway regulates calcium accumulation, permeability transition and cell death

Authors:

Wang Z, Liu D, Varin A, Nicolas V, Courilleau D, Mateo P, Caubere C, Rouet P, Gomez AM, V and ecasteele G, Fischmeister R, Brenner C.

Journal:

Cell Death Dis (2016): e2198

Spectral properties

Excitation (nm)	571
Emission (nm)	584

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black (Component H)

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on July 21, 2026