Screen Quest™ Fluorimetric ELISA cAMP Assay Kit

Screen Quest™ Fluorimetric ELISA cAMP Assay Kit is a competitive enzyme-linked immunosorbent assay for measuring cAMP using an HRP-mediated fluorescence readout.

Competitive cAMP detection: Sample cAMP competes with HRP-labeled cAMP for binding to immobilized anti-cAMP antibodies
Fluorogenic readout: Amplite® Red detects antibody-bound HRP-cAMP, with fluorescence decreasing as sample cAMP increases
Streamlined ELISA format: Requires no sample acetylation and uses one washing step before fluorescence development
Research applications: Suitable for measuring cAMP responses in GPCR signaling and evaluating compounds that modulate cellular cAMP levels

cAMP dose response was measured with Screen Quest™ Fluorimetric ELISA cAMP Assay Kit in a solid black 96- well plate with a Gemini microplate reader.

Protocol

Catalog	Size	Price	Quantity
36373	1 plate	Price
36374	10 plates	Price

Overview

Screen Quest™ Fluorimetric ELISA cAMP Assay Kit measures cAMP through competition between unlabeled sample cAMP and HRP-labeled cAMP for binding to immobilized anti-cAMP antibodies. Higher sample cAMP concentrations reduce the amount of antibody-bound HRP-cAMP. HRP catalyzes the oxidation of Amplite® Red in the presence of hydrogen peroxide to generate a fluorescent product, producing a signal that decreases as sample cAMP increases.

The kit uses an anti-cAMP antibody-coated 96-well plate, requires no sample acetylation, and includes one washing stage to remove unbound material before fluorescence development. Recommended fluorescence reader settings are Ex/Em = 540/590 nm, and cAMP concentrations are determined against a cAMP standard curve. The kit supports studies of GPCR-mediated cAMP signaling and compound effects on cellular cAMP levels.

Components

Catalog 36373 (1 plate)

Component A: cAMP Standard	1 vial (33 µg)
Component B: Assay Buffer	1 bottle (20 mL)
Component C: HRP-cAMP Conjugate	1 vial
Component D: 10X Wash Solution	1 bottle (10 mL)
Component E: Cell Lysis Buffer	1 bottle (10 mL)
Component F: 3% H2O2	1 vial (50 µL)
Component G: Amplite® Red	1 vial
Component H: Anti-cAMP Ab Coated 96-Well Plate	1 plate
Component I: Substrate Buffer	1 bottle (10 mL)

Catalog 36374 (10 plates)

Component A: cAMP Standard	1 vial (33 µg)
Component B: Assay Buffer	1 bottle (100 mL)
Component C: HRP-cAMP Conjugate	5 vials
Component D: 10X Wash Solution	1 bottle (100 mL)
Component E: Cell Lysis Buffer	1 bottle (100 mL)
Component F: 3% H2O2	1 vial (250 µL)
Component G: Amplite® Red	1 vial
Component H: Anti-cAMP Ab Coated 96-Well Plate	10 plates
Component I: Substrate Buffer	1 bottle (100 mL)

Citations

View all 5 citations: Citation Explorer

Propionate suppresses colitis-associated colorectal cancer in mice by Or51e2

Authors:

Kim, Ji-Sun and Jeong, Mi-Young and Yoon, Ye Eun and Kim, Yeonji and Lee, InRyeong and Choi, Jung-Won and Lee, Ha Lim and Shin, Min-Jeong and Hong, SungHoi and Chi, Sung-Gil and others,

Journal:

Biomedicine \& Pharmacotherapy (2025): 118268

OR2AT4, an Ectopic Olfactory Receptor, Suppresses Oxidative Stress-Induced Senescence in Human Keratinocytes

Authors:

Kim, Ji-Sun and Lee, Ha Lim and Jeong, Ji Hyun and Yoon, Ye Eun and Lee, In-Ryeong and Kim, Ji Min and Wu, Chunyan and Lee, Sung-Joon

Journal:

Antioxidants (2022): 2180

Albanin A, Derived from the Root Bark of Morus alba L., Depresses Glutamate Release in the Rat Cerebrocortical Nerve Terminals via Ca2+/Calmodulin/Adenylate Cyclase 1 Suppression

Authors:

Chang, Yi and Hung, Chi Feng and Ko, Horng Huey and Wang, Su Jane

Journal:

Journal of Medicinal Food (2021): 209--217

Activation of P2X7 and P2Y11 purinergic receptors inhibits migration and normalizes tumor-derived endothelial cells via cAMP signaling

Authors:

Avanzato, D and Genova, T and Pla, A Fiorio and Bernardini, M and Bianco, S and Bussolati, B and Mancardi, D and Giraudo, E and Maione, F and Cassoni, P and others, undefined

Journal:

Scientific Reports (2016)

The M2 muscarinic receptors are essential for signaling in the heart left ventricle during restraint stress in mice

Authors:

Tomankova, Hana and Valuskova, Paulina and Varejkova, Eva and Rotkova, Jana and Benes, Jan and Myslivecek, Jaromir

Journal:

Stress (2015)

References

View all 132 references: Citation Explorer

cAMP-Induced Histones H3 Dephosphorylation Is Independent of PKA and MAP Kinase Activations and Correlates With mTOR Inactivation

Authors:

Rodriguez P, Rojas J.

Journal:

J Cell Biochem (2016): 741

Changes in the Arabidopsis thaliana Proteome Implicate cAMP in Biotic and Abiotic Stress Responses and Changes in Energy Metabolism

Authors:

Alqurashi M, Gehring C, Marondedze C.

Journal:

Int J Mol Sci (2016): 852

Role of the cAMP Pathway in Glucose and Lipid Metabolism

Authors:

Ravnskjaer K, Madiraju A, Montminy M.

Journal:

Handb Exp Pharmacol (2016): 29

Odor-induced cAMP production in Drosophila melanogaster olfactory sensory neurons

Authors:

Miazzi F, Hansson BS, Wicher D.

Journal:

J Exp Biol (2016): 1798

A cardiac mitochondrial cAMP signaling pathway regulates calcium accumulation, permeability transition and cell death

Authors:

Wang Z, Liu D, Varin A, Nicolas V, Courilleau D, Mateo P, Caubere C, Rouet P, Gomez AM, V and ecasteele G, Fischmeister R, Brenner C.

Journal:

Cell Death Dis (2016): e2198

Spectral properties

Excitation (nm)	571
Emission (nm)	584

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black (Component H)

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Page updated on October 4, 2026