Screen Quest™ Fluorimetric ELISA cAMP Assay Kit

Screen Quest™ Fluorescence ELISA cAMP Assay Kit provides an optimized assay method for monitoring the activation of adenylyl cyclase in G-protein coupled receptor systems. The assay is based on the competition for a fixed number of antibody binding sites between HRP-labeled cAMP and non-labeled cAMP. HRP-cAMP is displaced from the HRP-cAMP/anti-cAMP antibody complex by unlabeled free cAMP. In the absence of cAMP, HRP-cAMP conjugate is bound to anti-cAMP antibody exclusively. However, the unlabeled free cAMP in the test sample competes for anti-cAMP antibody with the HRP-cAMP antibody conjugate, therefore inhibits the binding of HRP-cAMP to anti-cAMP antibody. Our Screen Quest™ Fluorometric cAMP Assay Kit provides a sensitive method for detecting adenylate cyclase activity. Compared to other commercial ELISA cAMP assay kits, this cAMP assay kit only requires a single wash step to remove unbound material prior to the development step. It also eliminates the tedious acetylation step. The kit uses Amplite® Red as a fluorogenic HRP substrate to quantify the HRP activity. The fluorescent product formed is proportional to the activity of HRP-cAMP conjugate.

Protocol

COA

Catalog	Size	Price	Quantity
36373	1 plate	Price
36374	10 plates	Price

Components

Catalog 36373 (1 plate)

Component A: cAMP Standard	1 vial (33 µg)
Component B: Assay Buffer	1 bottle (20 mL)
Component C: HRP-cAMP Conjugate	1 vial
Component D: 10X Wash Solution	1 bottle (10 mL)
Component E: Cell Lysis Buffer	1 bottle (10 mL)
Component F: 3% H2O2	1 vial (50 µL)
Component G: Amplite™ Red	1 vial
Component H: Anti-cAMP Ab Coated 96-Well Plate	1 plate
Component I: Substrate Buffer	1 bottle (10 mL)

Catalog 36374 (10 plates)

Component A: cAMP Standard	1 vial (33 µg)
Component B: Assay Buffer	1 bottle (100 mL)
Component C: HRP-cAMP Conjugate	5 vials
Component D: 10X Wash Solution	1 bottle (100 mL)
Component E: Cell Lysis Buffer	1 bottle (100 mL)
Component F: 3% H2O2	1 vial (250 µL)
Component G: Amplite™ Red	1 vial
Component H: Anti-cAMP Ab Coated 96-Well Plate	10 plates
Component I: Substrate Buffer	1 bottle (100 mL)

Citations

View all 5 citations: Citation Explorer

Propionate suppresses colitis-associated colorectal cancer in mice by Or51e2

Authors:

Kim, Ji-Sun and Jeong, Mi-Young and Yoon, Ye Eun and Kim, Yeonji and Lee, InRyeong and Choi, Jung-Won and Lee, Ha Lim and Shin, Min-Jeong and Hong, SungHoi and Chi, Sung-Gil and others,

Journal:

Biomedicine \& Pharmacotherapy (2025): 118268

OR2AT4, an Ectopic Olfactory Receptor, Suppresses Oxidative Stress-Induced Senescence in Human Keratinocytes

Authors:

Kim, Ji-Sun and Lee, Ha Lim and Jeong, Ji Hyun and Yoon, Ye Eun and Lee, In-Ryeong and Kim, Ji Min and Wu, Chunyan and Lee, Sung-Joon

Journal:

Antioxidants (2022): 2180

Albanin A, Derived from the Root Bark of Morus alba L., Depresses Glutamate Release in the Rat Cerebrocortical Nerve Terminals via Ca2+/Calmodulin/Adenylate Cyclase 1 Suppression

Authors:

Chang, Yi and Hung, Chi Feng and Ko, Horng Huey and Wang, Su Jane

Journal:

Journal of Medicinal Food (2021): 209--217

Activation of P2X7 and P2Y11 purinergic receptors inhibits migration and normalizes tumor-derived endothelial cells via cAMP signaling

Authors:

Avanzato, D and Genova, T and Pla, A Fiorio and Bernardini, M and Bianco, S and Bussolati, B and Mancardi, D and Giraudo, E and Maione, F and Cassoni, P and others, undefined

Journal:

Scientific Reports (2016)

The M2 muscarinic receptors are essential for signaling in the heart left ventricle during restraint stress in mice

Authors:

Tomankova, Hana and Valuskova, Paulina and Varejkova, Eva and Rotkova, Jana and Benes, Jan and Myslivecek, Jaromir

Journal:

Stress (2015)

References

View all 132 references: Citation Explorer

cAMP-Induced Histones H3 Dephosphorylation Is Independent of PKA and MAP Kinase Activations and Correlates With mTOR Inactivation

Authors:

Rodriguez P, Rojas J.

Journal:

J Cell Biochem (2016): 741

Changes in the Arabidopsis thaliana Proteome Implicate cAMP in Biotic and Abiotic Stress Responses and Changes in Energy Metabolism

Authors:

Alqurashi M, Gehring C, Marondedze C.

Journal:

Int J Mol Sci (2016): 852

Role of the cAMP Pathway in Glucose and Lipid Metabolism

Authors:

Ravnskjaer K, Madiraju A, Montminy M.

Journal:

Handb Exp Pharmacol (2016): 29

Odor-induced cAMP production in Drosophila melanogaster olfactory sensory neurons

Authors:

Miazzi F, Hansson BS, Wicher D.

Journal:

J Exp Biol (2016): 1798

A cardiac mitochondrial cAMP signaling pathway regulates calcium accumulation, permeability transition and cell death

Authors:

Wang Z, Liu D, Varin A, Nicolas V, Courilleau D, Mateo P, Caubere C, Rouet P, Gomez AM, V and ecasteele G, Fischmeister R, Brenner C.

Journal:

Cell Death Dis (2016): e2198

Spectral properties

Excitation (nm)	571
Emission (nm)	584

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black (Component H)

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 13, 2025