4-Azido-2,3,5,6-tetrafluorobenzoic Acid, Succinimidyl Ester
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Additional ordering information
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Physical properties
Molecular weight | 332.17 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Overview | SDSProtocol |
CAS 126695-58-7 | Molecular weight 332.17 |
Phenyl azides are a well-known class of photoaffinity probes that require photoactivation at short wavelengths (∼260 nm). Unfortunately, 260 nm UV causes damage to proteins, nucleic acids and other biological samples. This fluorinated phenyl azide can be effectively photoactivated at ∼300 nm, thus minimizing the photodamages caused by the short UV light. 4-Azido-2,3,5,6-tetrafluorobenzoic acid succinimidyl ester is an excellent building block to introduce photoaffinity function into proteins and other amino-containing biomolecules such as peptides and amino-modified oligos. Phenyl azides are known for their ability to undergo photochemical reactions when exposed to ultraviolet (UV) light, specifically by forming highly reactive carbene intermediates that react with nearby molecules, forming covalent bonds. The singlet nitrene intermediate formed on photoactivation is highly reactive. This property makes phenyl azides useful for studying protein-protein, protein-nucleic acid interactions, ligand-receptor binding, and other biomolecular interactions.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 4-Azido-2,3,5,6-tetrafluorobenzoic Acid, Succinimidyl Ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 301.051 µL | 1.505 mL | 3.011 mL | 15.053 mL | 30.105 mL |
5 mM | 60.21 µL | 301.051 µL | 602.101 µL | 3.011 mL | 6.021 mL |
10 mM | 30.105 µL | 150.525 µL | 301.051 µL | 1.505 mL | 3.011 mL |
Molarity calculator
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Images
Figure 1. 4-Azido-2,3,5,6-tetrafluorobenzoic acid succinimidyl ester is an excellent building block to introduce photoaffinity function into proteins and other amino-containing biomolecules such as peptides and amino-modified oligos. Phenyl azides are known for their ability to undergo photochemical reactions when exposed to ultraviolet (UV) light, specifically by forming highly reactive carbene intermediates that react with nearby molecules, forming covalent bonds. The singlet nitrene intermediate formed on photoactivation is highly reactive.
References
View all 7 references: Citation Explorer
Charting the Chemical and Mechanistic Scope of Light-Triggered Protein Ligation.
Authors: Earley, Daniel F and Guillou, Amaury and Klingler, Simon and Fay, Rachael and Gut, Melanie and d'Orchymont, Faustine and Behmaneshfar, Shamisa and Reichert, Linus and Holland, Jason P
Journal: JACS Au (2022): 646-664
Authors: Earley, Daniel F and Guillou, Amaury and Klingler, Simon and Fay, Rachael and Gut, Melanie and d'Orchymont, Faustine and Behmaneshfar, Shamisa and Reichert, Linus and Holland, Jason P
Journal: JACS Au (2022): 646-664
Asymmetric Photocatalysis with Bis-cyclometalated Rhodium Complexes.
Authors: Huang, Xiaoqiang and Meggers, Eric
Journal: Accounts of chemical research (2019): 833-847
Authors: Huang, Xiaoqiang and Meggers, Eric
Journal: Accounts of chemical research (2019): 833-847
The effect of photogenerated site-directed free radicals on surface dihydropyridine binding sites identified with photoaffinity probe (-)-[3H]-azidopine on cultured monkey renal cells.
Authors: Drímal, J and Bohácik, L
Journal: Methods and findings in experimental and clinical pharmacology (1994): 397-404
Authors: Drímal, J and Bohácik, L
Journal: Methods and findings in experimental and clinical pharmacology (1994): 397-404
Phenyl azide substituted and benzophenone-substituted phosphonamides of 7-methylguanosine 5'-triphosphate as photoaffinity probes for protein synthesis initiation factor eIF-4E and a proteolytic fragment containing the cap-binding site.
Authors: Chavan, A J and Rychlik, W and Blaas, D and Kuechler, E and Watt, D S and Rhoads, R E
Journal: Biochemistry (1990): 5521-9
Authors: Chavan, A J and Rychlik, W and Blaas, D and Kuechler, E and Watt, D S and Rhoads, R E
Journal: Biochemistry (1990): 5521-9
Heterobifunctional cross-linking agents incorporating perfluorinated aryl azides.
Authors: Crocker, P J and Imai, N and Rajagopalan, K and Boggess, M A and Kwiatkowski, S and Dwyer, L D and Vanaman, T C and Watt, D S
Journal: Bioconjugate chemistry (1990): 419-24
Authors: Crocker, P J and Imai, N and Rajagopalan, K and Boggess, M A and Kwiatkowski, S and Dwyer, L D and Vanaman, T C and Watt, D S
Journal: Bioconjugate chemistry (1990): 419-24
Detection of antimycin-binding subunits of complex III by photoaffinity-labeling with an azido derivative of antimycin.
Authors: Ho, S H and Das Gupta, U and Rieske, J S
Journal: Journal of bioenergetics and biomembranes (1985): 269-82
Authors: Ho, S H and Das Gupta, U and Rieske, J S
Journal: Journal of bioenergetics and biomembranes (1985): 269-82
Affinity labelling of isoelectrofocused fractions from a DNP antibody preparation with the photoactive labels 2,4-dinitrophenyl-1-azide and 2,4-dinitrophenyl-epsilon-aminocaproyldiazoketone.
Authors: Cannon, L E and Woodard, D K and Woehler, M E and Lovins, R E
Journal: Immunology (1974): 1183-94
Authors: Cannon, L E and Woodard, D K and Woehler, M E and Lovins, R E
Journal: Immunology (1974): 1183-94