Name: 4-Methylumbelliferyl-beta-D-glucoside *CAS 18997-57-4*
Brand: AAT Bioquest
SKU: 14014
Price: 90 USD
Availability: InStock

4-Methylumbelliferyl-beta-D-glucoside

CAS 18997-57-4

4-Methylumbelliferyl-beta-D-glucoside is a fluorogenic MU substrate for detecting glucosidase in cell extracts and purified enzyme preparations using a fluorescence microplate reader or fluorometer. The organic and inorganic esters and glycosides of 7-hydroxy-4-methylcoumarin (4-methylumbelliferone, MU) are among the most commonly used fluorogenic substrates. These substrates yield water-soluble blue fluorescent coumarin fluorophores. MU develops maximum fluorescence above pH 10.

Protocol

SDS

Catalog	Size	Price	Quantity
14014	25 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 4-Methylumbelliferyl-beta-D-glucoside *CAS 18997-57-4* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	295.578 µL	1.478 mL	2.956 mL	14.779 mL	29.558 mL
5 mM	59.116 µL	295.578 µL	591.156 µL	2.956 mL	5.912 mL
10 mM	29.558 µL	147.789 µL	295.578 µL	1.478 mL	2.956 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	338.32 g/mol	=	mL	x	mM	=	mmol

References

View all 5 references: Citation Explorer

Examination of lectins, polysaccharopeptide, polysaccharide, alkaloid, coumarin and trypsin inhibitors for inhibitory activity against human immunodeficiency virus reverse transcriptase and glycohydrolases

Authors:

Wang HX, Ng TB.

Journal:

Planta Med (2001): 669

Novel, lipophilic derivatives of 2,5-dideoxy-2,5-imino-D-mannitol (DMDP) are powerful beta-glucosidase inhibitors

Authors:

Wrodnigg TM, Withers SG, Stutz AE.

Journal:

Bioorg Med Chem Lett (2001): 1063

3-Hydroxycoumarins: first direct preparation from coumarins using a Cu2(+)-ascorbic acid-O2 system, and their potent bioactivities

Authors:

Aihara K, Higuchi T, Hirobe M.

Journal:

Biochem Biophys Res Commun (1990): 169

Subcellular Localization of 2-(beta-d-Glucosyloxy)-Cinnamic Acids and the Related beta-glucosidase in Leaves of Melilotus alba Desr

Authors:

Oba K, Conn EE, Canut H, Boudet AM.

Journal:

Plant Physiol (1981): 1359

Effect of coumarin on growth and metabolism of Pythium

Authors:

Neto FC, Dietrich SM.

Journal:

Appl Environ Microbiol (1977): 258

Physical properties

Molecular weight	338.32
Solvent	DMSO

Spectral properties

Excitation (nm)	341
Emission (nm)	441

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	18997-57-4

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Enzyme Probes & Assay Kits

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Page updated on October 28, 2025