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4-Methylumbelliferyl-beta-D-glucoside *CAS 18997-57-4*

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Physical properties
Molecular weight338.32
SolventDMSO
Spectral properties
Excitation (nm)341
Emission (nm)441
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


See also: Coumarins
CAS
18997-57-4
Molecular weight
338.32
Excitation (nm)
341
Emission (nm)
441
4-Methylumbelliferyl-beta-D-glucoside is a fluorogenic MU substrate for detecting glucosidase in cell extracts and purified enzyme preparations using a fluorescence microplate reader or fluorometer. The organic and inorganic esters and glycosides of 7-hydroxy-4-methylcoumarin (4-methylumbelliferone, MU) are among the most commonly used fluorogenic substrates. These substrates yield water-soluble blue fluorescent coumarin fluorophores. MU develops maximum fluorescence above pH 10.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 4-Methylumbelliferyl-beta-D-glucoside *CAS 18997-57-4* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM295.578 µL1.478 mL2.956 mL14.779 mL29.558 mL
5 mM59.116 µL295.578 µL591.156 µL2.956 mL5.912 mL
10 mM29.558 µL147.789 µL295.578 µL1.478 mL2.956 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Excitation (nm)341
Emission (nm)441

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References


View all 5 references: Citation Explorer
Novel, lipophilic derivatives of 2,5-dideoxy-2,5-imino-D-mannitol (DMDP) are powerful beta-glucosidase inhibitors
Authors: Wrodnigg TM, Withers SG, Stutz AE.
Journal: Bioorg Med Chem Lett (2001): 1063
Examination of lectins, polysaccharopeptide, polysaccharide, alkaloid, coumarin and trypsin inhibitors for inhibitory activity against human immunodeficiency virus reverse transcriptase and glycohydrolases
Authors: Wang HX, Ng TB.
Journal: Planta Med (2001): 669
3-Hydroxycoumarins: first direct preparation from coumarins using a Cu2(+)-ascorbic acid-O2 system, and their potent bioactivities
Authors: Aihara K, Higuchi T, Hirobe M.
Journal: Biochem Biophys Res Commun (1990): 169
Subcellular Localization of 2-(beta-d-Glucosyloxy)-Cinnamic Acids and the Related beta-glucosidase in Leaves of Melilotus alba Desr
Authors: Oba K, Conn EE, Canut H, Boudet AM.
Journal: Plant Physiol (1981): 1359
Effect of coumarin on growth and metabolism of Pythium
Authors: Neto FC, Dietrich SM.
Journal: Appl Environ Microbiol (1977): 258