4-Methylumbelliferyl-beta-D-glucoside *CAS 18997-57-4*
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 338.32 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 341 |
Emission (nm) | 441 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | SDSProtocol |
See also: Coumarins
CAS 18997-57-4 | Molecular weight 338.32 | Excitation (nm) 341 | Emission (nm) 441 |
4-Methylumbelliferyl-beta-D-glucoside is a fluorogenic MU substrate for detecting glucosidase in cell extracts and purified enzyme preparations using a fluorescence microplate reader or fluorometer. The organic and inorganic esters and glycosides of 7-hydroxy-4-methylcoumarin (4-methylumbelliferone, MU) are among the most commonly used fluorogenic substrates. These substrates yield water-soluble blue fluorescent coumarin fluorophores. MU develops maximum fluorescence above pH 10.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 4-Methylumbelliferyl-beta-D-glucoside *CAS 18997-57-4* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 295.578 µL | 1.478 mL | 2.956 mL | 14.779 mL | 29.558 mL |
5 mM | 59.116 µL | 295.578 µL | 591.156 µL | 2.956 mL | 5.912 mL |
10 mM | 29.558 µL | 147.789 µL | 295.578 µL | 1.478 mL | 2.956 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
References
View all 5 references: Citation Explorer
Novel, lipophilic derivatives of 2,5-dideoxy-2,5-imino-D-mannitol (DMDP) are powerful beta-glucosidase inhibitors
Authors: Wrodnigg TM, Withers SG, Stutz AE.
Journal: Bioorg Med Chem Lett (2001): 1063
Authors: Wrodnigg TM, Withers SG, Stutz AE.
Journal: Bioorg Med Chem Lett (2001): 1063
Examination of lectins, polysaccharopeptide, polysaccharide, alkaloid, coumarin and trypsin inhibitors for inhibitory activity against human immunodeficiency virus reverse transcriptase and glycohydrolases
Authors: Wang HX, Ng TB.
Journal: Planta Med (2001): 669
Authors: Wang HX, Ng TB.
Journal: Planta Med (2001): 669
3-Hydroxycoumarins: first direct preparation from coumarins using a Cu2(+)-ascorbic acid-O2 system, and their potent bioactivities
Authors: Aihara K, Higuchi T, Hirobe M.
Journal: Biochem Biophys Res Commun (1990): 169
Authors: Aihara K, Higuchi T, Hirobe M.
Journal: Biochem Biophys Res Commun (1990): 169
Subcellular Localization of 2-(beta-d-Glucosyloxy)-Cinnamic Acids and the Related beta-glucosidase in Leaves of Melilotus alba Desr
Authors: Oba K, Conn EE, Canut H, Boudet AM.
Journal: Plant Physiol (1981): 1359
Authors: Oba K, Conn EE, Canut H, Boudet AM.
Journal: Plant Physiol (1981): 1359
Effect of coumarin on growth and metabolism of Pythium
Authors: Neto FC, Dietrich SM.
Journal: Appl Environ Microbiol (1977): 258
Authors: Neto FC, Dietrich SM.
Journal: Appl Environ Microbiol (1977): 258
Application notes
A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets