5(6)-TRITC [Tetramethylrhodamine-5-(and-6)-isothiocyanate] *CAS 95197-95-8*

5(6)-TRITC

Tetramethylrhodamine-5-(and-6)-isothiocyanate; CAS 95197-95-8

5(6)-TRITC is an amino-reactive labeling reagent that is widely used in preparing bioconjugates of proteins and nucleic acids. The resultant conjugates have similar spectral properties to those prepared from 5(6)-TAMRA, SE. However, the latter conjugates are much more stable. Cautions must be exercised for the storage of TRITC conjugates.

Protocol

COO

COA

Catalog	Size	Price	Quantity
409	25 mg	Price
410	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5(6)-TRITC [Tetramethylrhodamine-5-(and-6)-isothiocyanate] *CAS 95197-95-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	208.342 µL	1.042 mL	2.083 mL	10.417 mL	20.834 mL
5 mM	41.668 µL	208.342 µL	416.684 µL	2.083 mL	4.167 mL
10 mM	20.834 µL	104.171 µL	208.342 µL	1.042 mL	2.083 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	479.98 g/mol	=	mL	x	mM	=	mmol

References

View all 45 references: Citation Explorer

Recombinant human erythropoietin prevents ischemia-induced apoptosis and necrosis in explant cultures of the rat organ of Corti

Authors:

Andreeva N, Nyamaa A, Haupt H, Gross J, Mazurek B.

Journal:

Neurosci Lett (2006): 86

Mechanism of advanced glycation end products-induced hyperpermeability in endothelial cells.

Authors:

Guo XH, Huang QB, Chen B, Wang SY, Hou FF, Fu N.

Journal:

Sheng Li Xue Bao (2005): 205

Musculature of an illoricate predatory rotifer Asplanchnopus multiceps as revealed by phalloidin fluorescence and confocal microscopy

Authors:

Kotikova EA, Raikova OI, Reuter M, Gustafsson MK.

Journal:

Tissue Cell (2004): 189

Resonance energy transfer nanobiosensors based on affinity binding between apo-enzyme and its substrate

Authors:

Chinnayelka S, McShane MJ.

Journal:

Biomacromolecules (2004): 1657

Nontypeable Haemophilus influenzae strain 2019 produces a biofilm containing N-acetylneuraminic acid that may mimic sialylated O-linked glycans

Authors:

Greiner LL, Watanabe H, Phillips NJ, Shao J, Morgan A, Zaleski A, Gibson BW, Apicella MA.

Journal:

Infect Immun (2004): 4249

Physical properties

Molecular weight	479.98
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.27
Correction factor (280 nm)	0.34
Extinction coefficient (cm ^-1 M ^-1)	100000
Excitation (nm)	544
Emission (nm)	570

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	95197-95-8

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Page updated on September 23, 2025