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5(6)-TRITC [Tetramethylrhodamine-5-(and-6)-isothiocyanate] *CAS 95197-95-8*

5(6)-TRITC is an amino-reactive labeling reagent that is widely used in preparing bioconjugates of proteins and nucleic acids. The resultant conjugates have similar spectral properties to those prepared from 5(6)-TAMRA, SE. However, the latter conjugates are much more stable. Cautions must be exercised for the storage of TRITC conjugates.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5(6)-TRITC [Tetramethylrhodamine-5-(and-6)-isothiocyanate] *CAS 95197-95-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM208.342 µL1.042 mL2.083 mL10.417 mL20.834 mL
5 mM41.668 µL208.342 µL416.684 µL2.083 mL4.167 mL
10 mM20.834 µL104.171 µL208.342 µL1.042 mL2.083 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
5(6)-CR6G [5-(and 6)-Carboxyrhodamine 6G]522546940000.240.214
5(6)-TAMRA [5(6)-Carboxytetramethylrhodamine] *CAS 98181-63-6*552578900000.320.178
5(6)-ROX [5-(and 6)-Carboxy-X-rhodamine] *CAS 198978-94-8*57860482000-0.168
5(6)-FAM [5-(and-6)-Carboxyfluorescein] *CAS 72088-94-9*493517830000.320.178

References

View all 45 references: Citation Explorer
Recombinant human erythropoietin prevents ischemia-induced apoptosis and necrosis in explant cultures of the rat organ of Corti
Authors: Andreeva N, Nyamaa A, Haupt H, Gross J, Mazurek B.
Journal: Neurosci Lett (2006): 86
Mechanism of advanced glycation end products-induced hyperpermeability in endothelial cells.
Authors: Guo XH, Huang QB, Chen B, Wang SY, Hou FF, Fu N.
Journal: Sheng Li Xue Bao (2005): 205
Musculature of an illoricate predatory rotifer Asplanchnopus multiceps as revealed by phalloidin fluorescence and confocal microscopy
Authors: Kotikova EA, Raikova OI, Reuter M, Gustafsson MK.
Journal: Tissue Cell (2004): 189
Resonance energy transfer nanobiosensors based on affinity binding between apo-enzyme and its substrate
Authors: Chinnayelka S, McShane MJ.
Journal: Biomacromolecules (2004): 1657
Nontypeable Haemophilus influenzae strain 2019 produces a biofilm containing N-acetylneuraminic acid that may mimic sialylated O-linked glycans
Authors: Greiner LL, Watanabe H, Phillips NJ, Shao J, Morgan A, Zaleski A, Gibson BW, Apicella MA.
Journal: Infect Immun (2004): 4249
Page updated on December 11, 2024

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25 mg
5 mg
Catalog Number
409410
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Physical properties

Molecular weight

479.98

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.27

Correction Factor (280 nm)

0.34

Extinction coefficient (cm -1 M -1)

100000

Excitation (nm)

544

Emission (nm)

570

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

CAS

95197-95-8