5(6)-TRITC [Tetramethylrhodamine-5-(and-6)-isothiocyanate] *CAS 95197-95-8*
5(6)-TRITC is an amino-reactive labeling reagent that is widely used in preparing bioconjugates of proteins and nucleic acids. The resultant conjugates have similar spectral properties to those prepared from 5(6)-TAMRA, SE. However, the latter conjugates are much more stable. Cautions must be exercised for the storage of TRITC conjugates.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 5(6)-TRITC [Tetramethylrhodamine-5-(and-6)-isothiocyanate] *CAS 95197-95-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 208.342 µL | 1.042 mL | 2.083 mL | 10.417 mL | 20.834 mL |
5 mM | 41.668 µL | 208.342 µL | 416.684 µL | 2.083 mL | 4.167 mL |
10 mM | 20.834 µL | 104.171 µL | 208.342 µL | 1.042 mL | 2.083 mL |
Molarity calculator
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Spectrum
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
5(6)-CR6G [5-(and 6)-Carboxyrhodamine 6G] | 522 | 546 | 94000 | 0.24 | 0.214 |
5(6)-TAMRA [5(6)-Carboxytetramethylrhodamine] *CAS 98181-63-6* | 552 | 578 | 90000 | 0.32 | 0.178 |
5(6)-ROX [5-(and 6)-Carboxy-X-rhodamine] *CAS 198978-94-8* | 578 | 604 | 82000 | - | 0.168 |
5(6)-FAM [5-(and-6)-Carboxyfluorescein] *CAS 72088-94-9* | 493 | 517 | 83000 | 0.32 | 0.178 |
References
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Journal: Biomacromolecules (2004): 1657
Nontypeable Haemophilus influenzae strain 2019 produces a biofilm containing N-acetylneuraminic acid that may mimic sialylated O-linked glycans
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