Alkyne Modifier I, NHS ester [PEG1 alkyne NHS ester]

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Physical properties

Molecular weight	225.20
Solvent	DMF

Storage, safety and handling

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Overview SDS Protocol

CAS

1174157-65-3

Molecular weight

225.20

Alkyne Modifier I, NHS ester (propargyl-NHS ester) is an alkyne-containing reagent that reacts with primary amines at pH 7-9 forming a stale amide bond. The terminal alkyne group reacts with azides by a mechanism known as the Cu-catalyzed click reaction enabling efficient and specific conjugation of derivatized molecules in biological samples. A short spacer arm adds minimal mass to modified molecules. It is widely used to introduce an alkynyl group into amino-modified oligonucleotides, peptides, proteins and other biological molecules.

Calculators

Common stock solution preparation

Table 1. Volume of DMF needed to reconstitute specific mass of Alkyne Modifier I, NHS ester [PEG1 alkyne NHS ester] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	444.05 µL	2.22 mL	4.44 mL	22.202 mL	44.405 mL
5 mM	88.81 µL	444.05 µL	888.099 µL	4.44 mL	8.881 mL
10 mM	44.405 µL	222.025 µL	444.05 µL	2.22 mL	4.44 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Images

Figure 1. Chemical structure for Alkyne Modifier I, NHS ester [PEG1 alkyne NHS ester].

Citations

View all 34 citations: Citation Explorer

Synthesis and Fabrication of Surface-Active Microparticles Using a Membrane Emulsion Technique and Conjugation of Model Protein via Strain-Promoted Azide-Alkyne Click Chemistry in Physiological Conditions
Authors: Walden, G., Liao, X., Riley, G., Donell, S., Raxworthy, M. J., Saeed, A.
Journal: Bioconjug Chem (2019): 531-535

Ultrasound-assisted Cu(I)-catalyzed azide-alkyne click cycloaddition as polymer-analogous transformation in chitosan chemistry. High antibacterial and transfection activity of novel triazol betaine chitosan derivatives and their nanoparticles
Authors: Kritchenkov, A. S., Egorov, A. R., Dysin, A. P., Volkova, O. V., Zabodalova, L. A., Suchkova, E. P., Kurliuk, A. V., Shakola, T. V.
Journal: Int J Biol Macromol (2019): 592-603

Injectable Catalyst-Free Poly(Propylene Fumarate) System Cross-Linked by Strain Promoted Alkyne-Azide Cycloaddition Click Chemistry for Spine Defect Filling
Authors: Liu, X., Miller, A. L., 2nd, Xu, H., Waletzki, B. E., Lu, L.
Journal: Biomacromolecules (2019): 3352-3365

Correction: CuAAC click chemistry for the enhanced detection of novel alkyne-based natural product toxins
Authors: Hems, E. S., Wagstaff, B. A., Saalbach, G., Field, R. A.
Journal: Chem Commun (Camb) (2018): 13161

Site-Specific Surface Functionalization via Microchannel Cantilever Spotting (microCS): Comparison between Azide-Alkyne and Thiol-Alkyne Click Chemistry Reactions
Authors: Dadfar, S. M. M., Sekula-Neuner, S., Bog, U., Trouillet, V., Hirtz, M.
Journal: Small (2018): e1800131

O-GlcNAcylation site mapping by (azide-alkyne) click chemistry and mass spectrometry following intensive fractionation of skeletal muscle cells proteins
Authors: Deracinois, B., Camoin, L., Lambert, M., Boyer, J. B., Dupont, E., Bastide, B., Cieniewski-Bernard, C.
Journal: J Proteomics (2018): 83-97

Ribosome-Templated Azide-Alkyne Cycloadditions Using Resistant Bacteria as Reaction Vessels: in Cellulo Click Chemistry
Authors: Jin, X., Daher, S. S., Lee, M., Buttaro, B., Andrade, R. B.
Journal: ACS Med Chem Lett (2018): 907-911

CuAAC click chemistry for the enhanced detection of novel alkyne-based natural product toxins
Authors: Hems, E. S., Wagstaff, B. A., Saalbach, G., Field, R. A.
Journal: Chem Commun (Camb) (2018): 12234-12237

Detection of Alkynes via Click Chemistry with a Brominated Coumarin Azide by Simultaneous Fluorescence and Isotopic Signatures in Mass Spectrometry
Authors: Yang, L., Chumsae, C., Kaplan, J. B., Moulton, K. R., Wang, D., Lee, D. H., Zhou, Z. S.
Journal: Bioconjug Chem (2017): 2302-2309

Synthesis of Polystyrene and Poly(4-vinylpyridine) Mixed Grafted Silica Nanoparticles via a Combination of ATRP and Cu(I) -Catalyzed Azide-Alkyne Click Chemistry
Authors: Wu, L., Glebe, U., Boker, A.
Journal: Macromol Rapid Commun (2017): se name="4531.enl" path="C:\Users\aatbi\Dropb