Name: Alkyne Modifier I, NHS ester [PEG1 alkyne NHS ester]
Brand: AAT Bioquest
SKU: 4531
Price: 115 USD
Availability: InStock

Alkyne Modifier I, NHS ester

PEG1 alkyne NHS ester

Alkyne Modifier I, NHS ester (propargyl-NHS ester) is an alkyne-containing reagent that reacts with primary amines at pH 7-9 forming a stale amide bond. The terminal alkyne group reacts with azides by a mechanism known as the Cu-catalyzed click reaction enabling efficient and specific conjugation of derivatized molecules in biological samples. A short spacer arm adds minimal mass to modified molecules. It is widely used to introduce an alkynyl group into amino-modified oligonucleotides, peptides, proteins and other biological molecules.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
4531	25 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMF needed to reconstitute specific mass of Alkyne Modifier I, NHS ester [PEG1 alkyne NHS ester] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	444.05 µL	2.22 mL	4.44 mL	22.202 mL	44.405 mL
5 mM	88.81 µL	444.05 µL	888.099 µL	4.44 mL	8.881 mL
10 mM	44.405 µL	222.025 µL	444.05 µL	2.22 mL	4.44 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	225.2 g/mol	=	mL	x	mM	=	mmol

Citations

View all 34 citations: Citation Explorer

Synthesis and Fabrication of Surface-Active Microparticles Using a Membrane Emulsion Technique and Conjugation of Model Protein via Strain-Promoted Azide-Alkyne Click Chemistry in Physiological Conditions

Authors:

Walden, G., Liao, X., Riley, G., Donell, S., Raxworthy, M. J., Saeed, A.

Journal:

Bioconjug Chem (2019): 531-535

Ultrasound-assisted Cu(I)-catalyzed azide-alkyne click cycloaddition as polymer-analogous transformation in chitosan chemistry. High antibacterial and transfection activity of novel triazol betaine chitosan derivatives and their nanoparticles

Authors:

Kritchenkov, A. S., Egorov, A. R., Dysin, A. P., Volkova, O. V., Zabodalova, L. A., Suchkova, E. P., Kurliuk, A. V., Shakola, T. V.

Journal:

Int J Biol Macromol (2019): 592-603

Injectable Catalyst-Free Poly(Propylene Fumarate) System Cross-Linked by Strain Promoted Alkyne-Azide Cycloaddition Click Chemistry for Spine Defect Filling

Authors:

Liu, X., Miller, A. L., 2nd, Xu, H., Waletzki, B. E., Lu, L.

Journal:

Biomacromolecules (2019): 3352-3365

CuAAC click chemistry for the enhanced detection of novel alkyne-based natural product toxins

Authors:

Hems, E. S., Wagstaff, B. A., Saalbach, G., Field, R. A.

Journal:

Chem Commun (Camb) (2018): 12234-12237

Ribosome-Templated Azide-Alkyne Cycloadditions Using Resistant Bacteria as Reaction Vessels: in Cellulo Click Chemistry

Authors:

Jin, X., Daher, S. S., Lee, M., Buttaro, B., Andrade, R. B.

Journal:

ACS Med Chem Lett (2018): 907-911

Physical properties

Molecular weight	225.20
Solvent	DMF

Storage, safety and handling

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure
CAS	1174157-65-3

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 19, 2025