Alkyne Modifier II, NHS ester [PEG4 alkyne NHS ester]

Ordering information

Price
Catalog Number
Unit Size
Quantity

Add to cart

Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	401.41
Solvent	DMF

Storage, safety and handling

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Overview SDS Protocol

CAS

1393330-40-9

Molecular weight

401.41

Alkyne Modifier II, NHS ester is an alkyne-containing reagent that reacts with primary amines at pH 7-9 forming a stale amide bond. The terminal alkyne group reacts with azides by a mechanism known as the Cu-catalyzed click reaction enabling efficient and specific conjugation of derivatized molecules in biological samples. It is widely used to introduce an alkynyl group into amino-modified oligonucleotides, peptides, proteins and other biological molecules. Compared to Alkyne Modifier I, NHS ester (4531), it adds a longer PEG4 spacer arm to the modified molecules.

Calculators

Common stock solution preparation

Table 1. Volume of DMF needed to reconstitute specific mass of Alkyne Modifier II, NHS ester [PEG4 alkyne NHS ester] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	249.122 µL	1.246 mL	2.491 mL	12.456 mL	24.912 mL
5 mM	49.824 µL	249.122 µL	498.244 µL	2.491 mL	4.982 mL
10 mM	24.912 µL	124.561 µL	249.122 µL	1.246 mL	2.491 mL