Alkyne Modifier II, NHS ester [PEG4 alkyne NHS ester]

Alkyne Modifier II, NHS ester

PEG4 alkyne NHS ester

Alkyne Modifier II, NHS ester is an alkyne-containing reagent that reacts with primary amines at pH 7-9 forming a stale amide bond. The terminal alkyne group reacts with azides by a mechanism known as the Cu-catalyzed click reaction enabling efficient and specific conjugation of derivatized molecules in biological samples. It is widely used to introduce an alkynyl group into amino-modified oligonucleotides, peptides, proteins and other biological molecules. Compared to Alkyne Modifier I, NHS ester (4531), it adds a longer PEG4 spacer arm to the modified molecules.

General packaging for Cat. No. 4533. Your actual packaging may differ.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
4533	25 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMF needed to reconstitute specific mass of Alkyne Modifier II, NHS ester [PEG4 alkyne NHS ester] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	249.122 µL	1.246 mL	2.491 mL	12.456 mL	24.912 mL
5 mM	49.824 µL	249.122 µL	498.244 µL	2.491 mL	4.982 mL
10 mM	24.912 µL	124.561 µL	249.122 µL	1.246 mL	2.491 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	401.41 g/mol	=	mL	x	mM	=	mmol

Physical properties

Molecular weight	401.41
Solvent	DMF

Storage, safety and handling

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure
CAS	1393330-40-9

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on November 5, 2025