Name: 5(6)-CR6G [5-(and 6)-Carboxyrhodamine 6G]
Brand: AAT Bioquest
SKU: 330
Price: 232 USD
Availability: InStock

5(6)-CR6G

5-(and 6)-Carboxyrhodamine 6G

In general, rhodamine 6G conjugates have higher fluorescence quantum yields than tetramethylrhodamine conjugates, as well as good photostability. 5(6)-CR6G is the mixture of two carboxy rhodamine 6G isomers. It is used to modify amino and hydroxy groups using EDC-mediated couplings when there are difficulties in using 5(6)-CR6G, SE. The excitation and emission wavelengths of rhodamine 6G fall between those of fluorescein and tetramethylrhodamine derivatives, making 5(6)-CR6G another color choice for the multicolor fluorescence imaging applications.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
330	25 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5(6)-CR6G [5-(and 6)-Carboxyrhodamine 6G] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	202.032 µL	1.01 mL	2.02 mL	10.102 mL	20.203 mL
5 mM	40.406 µL	202.032 µL	404.065 µL	2.02 mL	4.041 mL
10 mM	20.203 µL	101.016 µL	202.032 µL	1.01 mL	2.02 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	494.97 g/mol	=	mL	x	mM	=	mmol

Citations

View all 2 citations: Citation Explorer

Simultaneous membrane interaction of amphipathic peptide monomers, self-aggregates and cargo complexes detected by fluorescence correlation spectroscopy

Authors:

Vasconcelos, Luis and Lehto, T{\~o}nis and Madani, Fatemeh and Radoi, Vlad and H{\"a}llbrink, Mattias and Vukojevi{\'c}, Vladana and Langel, {\"U}lo

Journal:

Biochimica et Biophysica Acta (BBA)-Biomembranes (2018): 491--504

Rotational motion of rhodamine 6G tethered to actin through oligo (ethylene glycol) linkers studied by frequency-domain fluorescence anisotropy

Authors:

Wazawa, Tetsuichi and Morimoto, Nobuyuki and Nagai, Takeharu and Suzuki, Makoto

Journal:

Biophysics and Physicobiology (2015): 87--102

References

View all 6 references: Citation Explorer

Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides

Authors:

Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.

Journal:

Proc Natl Acad Sci U S A (2005): 5926

Modifying the adsorption behavior of polyamidoamine dendrimers at silica surfaces investigated by total internal reflection fluorescence correlation spectroscopy

Authors:

McCain KS, Schluesche P, Harris JM.

Journal:

Anal Chem (2004): 930

High-power blue/UV light-emitting diodes as excitation sources for sensitive detection

Authors:

Kuo JS, Kuyper CL, Allen PB, Fiorini GS, Chiu DT.

Journal:

Electrophoresis (2004): 3796

Fluorescence resonance energy transfer (FRET) and competing processes in donor-acceptor substituted DNA strands: a comparative study of ensemble and single-molecule data

Authors:

Dietrich A, Buschmann V, Muller C, Sauer M.

Journal:

J Biotechnol (2002): 211

Efficient and high fidelity incorporation of dye-terminators by a novel archaeal DNA polymerase mutant

Authors:

Arezi B, Hansen CJ, Hogrefe HH.

Journal:

J Mol Biol (2002): 719

Physical properties

Molecular weight	494.97
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.24
Correction factor (280 nm)	0.214
Extinction coefficient (cm ^-1 M ^-1)	94000
Excitation (nm)	522
Emission (nm)	546

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 12, 2025