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5(6)-CR6G [5-(and 6)-Carboxyrhodamine 6G]

In general, rhodamine 6G conjugates have higher fluorescence quantum yields than tetramethylrhodamine conjugates, as well as good photostability. 5(6)-CR6G is the mixture of two carboxy rhodamine 6G isomers. It is used to modify amino and hydroxy groups using EDC-mediated couplings when there are difficulties in using 5(6)-CR6G, SE. The excitation and emission wavelengths of rhodamine 6G fall between those of fluorescein and tetramethylrhodamine derivatives, making 5(6)-CR6G another color choice for the multicolor fluorescence imaging applications.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5(6)-CR6G [5-(and 6)-Carboxyrhodamine 6G] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM202.032 µL1.01 mL2.02 mL10.102 mL20.203 mL
5 mM40.406 µL202.032 µL404.065 µL2.02 mL4.041 mL
10 mM20.203 µL101.016 µL202.032 µL1.01 mL2.02 mL

Molarity calculator

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Spectrum

Citations

View all 2 citations: Citation Explorer
Simultaneous membrane interaction of amphipathic peptide monomers, self-aggregates and cargo complexes detected by fluorescence correlation spectroscopy
Authors: Vasconcelos, Luis and Lehto, T{\~o}nis and Madani, Fatemeh and Radoi, Vlad and H{\"a}llbrink, Mattias and Vukojevi{\'c}, Vladana and Langel, {\"U}lo
Journal: Biochimica et Biophysica Acta (BBA)-Biomembranes (2018): 491--504
Rotational motion of rhodamine 6G tethered to actin through oligo (ethylene glycol) linkers studied by frequency-domain fluorescence anisotropy
Authors: Wazawa, Tetsuichi and Morimoto, Nobuyuki and Nagai, Takeharu and Suzuki, Makoto
Journal: Biophysics and Physicobiology (2015): 87--102

References

View all 6 references: Citation Explorer
Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926
Modifying the adsorption behavior of polyamidoamine dendrimers at silica surfaces investigated by total internal reflection fluorescence correlation spectroscopy
Authors: McCain KS, Schluesche P, Harris JM.
Journal: Anal Chem (2004): 930
High-power blue/UV light-emitting diodes as excitation sources for sensitive detection
Authors: Kuo JS, Kuyper CL, Allen PB, Fiorini GS, Chiu DT.
Journal: Electrophoresis (2004): 3796
Fluorescence resonance energy transfer (FRET) and competing processes in donor-acceptor substituted DNA strands: a comparative study of ensemble and single-molecule data
Authors: Dietrich A, Buschmann V, Muller C, Sauer M.
Journal: J Biotechnol (2002): 211
Efficient and high fidelity incorporation of dye-terminators by a novel archaeal DNA polymerase mutant
Authors: Arezi B, Hansen CJ, Hogrefe HH.
Journal: J Mol Biol (2002): 719
Page updated on December 11, 2024

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Physical properties

Molecular weight

494.97

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.24

Correction Factor (280 nm)

0.214

Extinction coefficient (cm -1 M -1)

94000

Excitation (nm)

522

Emission (nm)

546

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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