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5(6)-CR6G, SE [5-(and 6)-Carboxyrhodamine 6G, succinimidyl ester] *Mixed isomers*

Chemical structure for 5(6)-CR6G, SE [5-(and 6)-Carboxyrhodamine 6G, succinimidyl ester] *Mixed isomers*
Chemical structure for 5(6)-CR6G, SE [5-(and 6)-Carboxyrhodamine 6G, succinimidyl ester] *Mixed isomers*
Ordering information
Price ()Discontinued
Catalog Number340
Unit Size
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Additional ordering information
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Fax1-408-733-1304
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ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight555.59
SolventDMSO
Spectral properties
Correction Factor (260 nm)0.24
Correction Factor (280 nm)0.214
Extinction coefficient (cm -1 M -1)94000
Excitation (nm)522
Emission (nm)546
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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5(6)-CR6G [5-(and 6)-Carboxyrhodamine 6G]
5-CR6G [5-Carboxyrhodamine 6G] *Single isomer*
6-CR6G [6-Carboxyrhodamine 6G] *Single isomer*
6-CR6G, SE [6-Carboxyrhodamine 6G, succinimidyl ester] *Single isomer*
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Rhodamine aldehyde [5-TAMRA aldehyde]
Show More (55)

OverviewpdfSDSpdfProtocol


Molecular weight
555.59
Correction Factor (260 nm)
0.24
Correction Factor (280 nm)
0.214
Extinction coefficient (cm -1 M -1)
94000
Excitation (nm)
522
Emission (nm)
546
The absorption maxima of CR6G conjugates match well with the 514 nm spectral line of the argon-ion laser that is used in most of fluorescence instruments. In addition, the excitation and emission peaks of rhodamine 6G conjugates falling between those of fluorescein and tetramethylrhodamine provide another color choice for the multicolor imaging applications. 5(6)-CR6G, SE is the amine-reactive form of 5(6)-CR6G. Although 5(6)-CR6G, SE has excellent fluorescence properties, it has very poor solubility in buffers, DMSO or DMF, limiting its utility. iFluor® 514 has the essentially same spectral properties to 5(6)-CR6G, making iFluor® 514 an excellent replacement for 5(6)-CR6G.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5(6)-CR6G, SE [5-(and 6)-Carboxyrhodamine 6G, succinimidyl ester] *Mixed isomers* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM179.989 µL899.944 µL1.8 mL8.999 mL17.999 mL
5 mM35.998 µL179.989 µL359.978 µL1.8 mL3.6 mL
10 mM17.999 µL89.994 µL179.989 µL899.944 µL1.8 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Correction Factor (260 nm)0.24
Correction Factor (280 nm)0.214
Extinction coefficient (cm -1 M -1)94000
Excitation (nm)522
Emission (nm)546

Product family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
5(6)-TAMRA, SE [5-(and-6)-Carboxytetramethylrhodamine, succinimidyl ester] *CAS 246256-50-8*552578900000.320.178
5(6)-ROX, SE [5-(and-6)-Carboxy-X-rhodamine, succinimidyl ester] *Mixed isomers*57860482000-0.168
5(6)-FAM, SE [5-(and-6)-Carboxyfluorescein, succinimidyl ester] *CAS 117548-22-8*493517830000.320.178

Citations


View all 1 citations: Citation Explorer
Rotational motion of rhodamine 6G tethered to actin through oligo (ethylene glycol) linkers studied by frequency-domain fluorescence anisotropy
Authors: Wazawa, Tetsuichi and Morimoto, Nobuyuki and Nagai, Takeharu and Suzuki, Makoto
Journal: Biophysics and Physicobiology (2015): 87--102

References


View all 6 references: Citation Explorer
Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926
High-power blue/UV light-emitting diodes as excitation sources for sensitive detection
Authors: Kuo JS, Kuyper CL, Allen PB, Fiorini GS, Chiu DT.
Journal: Electrophoresis (2004): 3796
Modifying the adsorption behavior of polyamidoamine dendrimers at silica surfaces investigated by total internal reflection fluorescence correlation spectroscopy
Authors: McCain KS, Schluesche P, Harris JM.
Journal: Anal Chem (2004): 930
Efficient and high fidelity incorporation of dye-terminators by a novel archaeal DNA polymerase mutant
Authors: Arezi B, Hansen CJ, Hogrefe HH.
Journal: J Mol Biol (2002): 719
Fluorescence resonance energy transfer (FRET) and competing processes in donor-acceptor substituted DNA strands: a comparative study of ensemble and single-molecule data
Authors: Dietrich A, Buschmann V, Muller C, Sauer M.
Journal: J Biotechnol (2002): 211
Optimization of spectroscopic and electrophoretic properties of energy transfer primers
Authors: Hung SC, Mathies RA, Glazer AN.
Journal: Anal Biochem (1997): 78