5(6)-CR6G, SE [5-(and 6)-Carboxyrhodamine 6G, succinimidyl ester] Mixed isomers

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Physical properties

Molecular weight	555.59
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.24
Correction Factor (280 nm)	0.214
Extinction coefficient (cm ^-1 M ^-1)	94000
Excitation (nm)	522
Emission (nm)	546

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

555.59

Correction Factor (260 nm)

0.24

Correction Factor (280 nm)

0.214

Extinction coefficient (cm ^-1 M ^-1)

94000

Excitation (nm)

522

Emission (nm)

546

The absorption maxima of CR6G conjugates match well with the 514 nm spectral line of the argon-ion laser that is used in most of fluorescence instruments. In addition, the excitation and emission peaks of rhodamine 6G conjugates falling between those of fluorescein and tetramethylrhodamine provide another color choice for the multicolor imaging applications. 5(6)-CR6G, SE is the amine-reactive form of 5(6)-CR6G. Although 5(6)-CR6G, SE has excellent fluorescence properties, it has very poor solubility in buffers, DMSO or DMF, limiting its utility. iFluor® 514 has the essentially same spectral properties to 5(6)-CR6G, making iFluor® 514 an excellent replacement for 5(6)-CR6G.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5(6)-CR6G, SE [5-(and 6)-Carboxyrhodamine 6G, succinimidyl ester] *Mixed isomers* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	179.989 µL	899.944 µL	1.8 mL	8.999 mL	17.999 mL
5 mM	35.998 µL	179.989 µL	359.978 µL	1.8 mL	3.6 mL
10 mM	17.999 µL	89.994 µL	179.989 µL	899.944 µL	1.8 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (260 nm)	0.24
Correction Factor (280 nm)	0.214
Extinction coefficient (cm ^-1 M ^-1)	94000
Excitation (nm)	522
Emission (nm)	546

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (260 nm)	Correction Factor (280 nm)
5(6)-TAMRA, SE [5-(and-6)-Carboxytetramethylrhodamine, succinimidyl ester] CAS 246256-50-8	552	578	90000	0.32	0.178
5(6)-ROX, SE [5-(and-6)-Carboxy-X-rhodamine, succinimidyl ester] Mixed isomers	578	604	82000	-	0.168
5(6)-FAM, SE [5-(and-6)-Carboxyfluorescein, succinimidyl ester] CAS 117548-22-8	493	517	83000	0.32	0.178

Images

Figure 1. Chemical structure for 5(6)-CR6G, SE [5-(and 6)-Carboxyrhodamine 6G, succinimidyl ester] *Mixed isomers*

Citations

View all 1 citations: Citation Explorer

Rotational motion of rhodamine 6G tethered to actin through oligo (ethylene glycol) linkers studied by frequency-domain fluorescence anisotropy
Authors: Wazawa, Tetsuichi and Morimoto, Nobuyuki and Nagai, Takeharu and Suzuki, Makoto
Journal: Biophysics and Physicobiology (2015): 87--102

References

View all 6 references: Citation Explorer

Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926

High-power blue/UV light-emitting diodes as excitation sources for sensitive detection
Authors: Kuo JS, Kuyper CL, Allen PB, Fiorini GS, Chiu DT.
Journal: Electrophoresis (2004): 3796

Modifying the adsorption behavior of polyamidoamine dendrimers at silica surfaces investigated by total internal reflection fluorescence correlation spectroscopy
Authors: McCain KS, Schluesche P, Harris JM.
Journal: Anal Chem (2004): 930

Efficient and high fidelity incorporation of dye-terminators by a novel archaeal DNA polymerase mutant
Authors: Arezi B, Hansen CJ, Hogrefe HH.
Journal: J Mol Biol (2002): 719

Fluorescence resonance energy transfer (FRET) and competing processes in donor-acceptor substituted DNA strands: a comparative study of ensemble and single-molecule data
Authors: Dietrich A, Buschmann V, Muller C, Sauer M.
Journal: J Biotechnol (2002): 211

Optimization of spectroscopic and electrophoretic properties of energy transfer primers
Authors: Hung SC, Mathies RA, Glazer AN.
Journal: Anal Biochem (1997): 78