5(6)-ROX [5-(and 6)-Carboxy-X-rhodamine] CAS 198978-94-8

Ordering information

Price
Catalog Number
Unit Size
Quantity

Add to cart

Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	534.60
Solvent	DMSO

Spectral properties

Correction Factor (280 nm)	0.168
Extinction coefficient (cm ^-1 M ^-1)	82000
Excitation (nm)	578
Emission (nm)	604
Quantum yield	0.94¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

198978-94-8

Molecular weight

534.60

Correction Factor (280 nm)

0.168

Extinction coefficient (cm ^-1 M ^-1)

82000

Excitation (nm)

578

Emission (nm)

604

Quantum yield

0.94¹

ROX dyes are strongly red fluorescent. They have longer excitation and emission wavelengths than the 'conventional' rhodamines. These dyes are used to label peptides, proteins, and other biological ligands. 5-ROX, 6-ROX and their mixture 5(6)-ROX are used to label biomolecules by EDC-mediated reactions. The ratio of 6-isomer/5-isomer is in the range 3/1 to 6/1. Compared to other rhodamines, ROX is very unstable. Cautions must be exercised to prevent the deterioration during storage.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5(6)-ROX [5-(and 6)-Carboxy-X-rhodamine] *CAS 198978-94-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	187.056 µL	935.279 µL	1.871 mL	9.353 mL	18.706 mL
5 mM	37.411 µL	187.056 µL	374.111 µL	1.871 mL	3.741 mL
10 mM	18.706 µL	93.528 µL	187.056 µL	935.279 µL	1.871 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (280 nm)	0.168
Extinction coefficient (cm ^-1 M ^-1)	82000
Excitation (nm)	578
Emission (nm)	604
Quantum yield	0.94¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (280 nm)
5(6)-CR6G [5-(and 6)-Carboxyrhodamine 6G]	522	546	94000	-	0.214
5(6)-TAMRA [5(6)-Carboxytetramethylrhodamine] CAS 98181-63-6	552	578	90000	-	0.178
5-ROX, SE [5-Carboxy-X-rhodamine, succinimidyl ester] CAS 209734-74-7	578	604	82000	0.94¹	0.168
6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester] CAS#: 216699-36-4	578	604	82000	0.94¹	0.168
5(6)-TRITC [Tetramethylrhodamine-5-(and-6)-isothiocyanate] CAS 95197-95-8	544	570	100000	-	0.34
5(6)-FAM [5-(and-6)-Carboxyfluorescein] CAS 72088-94-9	493	517	83000	-	0.178
6-ROX glycine 25 uM fluorescence reference solution for PCR reactions	578	604	82000	0.94¹	0.168
5-ROX glycine Fluorescence reference standard for PCR reactions	578	604	82000	0.94¹	0.168
6-ROX azide	578	604	82000	0.94¹	0.168
6-ROX alkyne	578	604	82000	0.94¹	0.168
Show More (1)

Images

Figure 1. Chemical structure for 5(6)-ROX [5-(and 6)-Carboxy-X-rhodamine] *CAS 198978-94-8*

References

View all 11 references: Citation Explorer

Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926

Adsorption of oligonucleotides on PMMA/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence
Authors: Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C.
Journal: Langmuir (2004): 6834

Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry
Authors: Seo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2004): 5488

Evaluation of surface-enhanced resonance Raman scattering for quantitative DNA analysis
Authors: Faulds K, Smith WE, Graham D.
Journal: Anal Chem (2004): 412

Blue light-induced generation of reactive oxygen species in photoreceptor ellipsoids requires mitochondrial electron transport
Authors: Yang JH, Basinger SF, Gross RL, Wu SM.
Journal: Invest Ophthalmol Vis Sci (2003): 1312

HLA-DRB fluorotyping by dark quenching and automated analysis
Authors: Slateva K, Elsner HA, Albis-Camps M, Blasczyk R.
Journal: Tissue Antigens (2001): 250

Influence of fluorophor dye labels on the migration behavior of polymerase chain reaction--amplified short tandem repeats during denaturing capillary electrophoresis
Authors: Hahn M, Wilhelm J, Pingoud A.
Journal: Electrophoresis (2001): 2691

Design, synthesis, and spectroscopic properties of peptide-bridged fluorescence energy-transfer cassettes
Authors: Li Y, Glazer AN.
Journal: Bioconjug Chem (1999): 241

Differential display with carboxy-X-rhodamine-labeled primers and the selection of differentially amplified cDNA fragments without cloning
Authors: Yoshikawa Y, Mukai H, Asada K, Hino F, Kato I.
Journal: Anal Biochem (1998): 82

Comparison of fluorescence energy transfer primers with different donor-acceptor dye combinations
Authors: Hung SC, Mathies RA, Glazer AN.
Journal: Anal Biochem (1998): 32