5(6)-ROX [5-(and 6)-Carboxy-X-rhodamine] *CAS 198978-94-8*
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Physical properties
Molecular weight | 534.60 |
Solvent | DMSO |
Spectral properties
Correction Factor (280 nm) | 0.168 |
Extinction coefficient (cm -1 M -1) | 82000 |
Excitation (nm) | 578 |
Emission (nm) | 604 |
Quantum yield | 0.941 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
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See also: Rhodamines and Rhodamine Derivatives
CAS 198978-94-8 | Molecular weight 534.60 | Correction Factor (280 nm) 0.168 | Extinction coefficient (cm -1 M -1) 82000 | Excitation (nm) 578 | Emission (nm) 604 | Quantum yield 0.941 |
ROX dyes are strongly red fluorescent. They have longer excitation and emission wavelengths than the 'conventional' rhodamines. These dyes are used to label peptides, proteins, and other biological ligands. 5-ROX, 6-ROX and their mixture 5(6)-ROX are used to label biomolecules by EDC-mediated reactions. The ratio of 6-isomer/5-isomer is in the range 3/1 to 6/1. Compared to other rhodamines, ROX is very unstable. Cautions must be exercised to prevent the deterioration during storage.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 5(6)-ROX [5-(and 6)-Carboxy-X-rhodamine] *CAS 198978-94-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 187.056 µL | 935.279 µL | 1.871 mL | 9.353 mL | 18.706 mL |
5 mM | 37.411 µL | 187.056 µL | 374.111 µL | 1.871 mL | 3.741 mL |
10 mM | 18.706 µL | 93.528 µL | 187.056 µL | 935.279 µL | 1.871 mL |
Molarity calculator
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Spectrum
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Spectral properties
Correction Factor (280 nm) | 0.168 |
Extinction coefficient (cm -1 M -1) | 82000 |
Excitation (nm) | 578 |
Emission (nm) | 604 |
Quantum yield | 0.941 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (280 nm) |
5(6)-CR6G [5-(and 6)-Carboxyrhodamine 6G] | 522 | 546 | 94000 | - | 0.214 |
5(6)-TAMRA [5(6)-Carboxytetramethylrhodamine] *CAS 98181-63-6* | 552 | 578 | 90000 | - | 0.178 |
5-ROX, SE [5-Carboxy-X-rhodamine, succinimidyl ester] *CAS 209734-74-7* | 578 | 604 | 82000 | 0.941 | 0.168 |
6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester] *CAS#: 216699-36-4* | 578 | 604 | 82000 | 0.941 | 0.168 |
5(6)-TRITC [Tetramethylrhodamine-5-(and-6)-isothiocyanate] *CAS 95197-95-8* | 544 | 570 | 100000 | - | 0.34 |
5(6)-FAM [5-(and-6)-Carboxyfluorescein] *CAS 72088-94-9* | 493 | 517 | 83000 | - | 0.178 |
6-ROX glycine *25 uM fluorescence reference solution for PCR reactions* | 578 | 604 | 82000 | 0.941 | 0.168 |
5-ROX glycine *Fluorescence reference standard for PCR reactions* | 578 | 604 | 82000 | 0.941 | 0.168 |
6-ROX azide | 578 | 604 | 82000 | 0.941 | 0.168 |
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References
View all 11 references: Citation Explorer
Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926
Adsorption of oligonucleotides on PMMA/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence
Authors: Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C.
Journal: Langmuir (2004): 6834
Authors: Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C.
Journal: Langmuir (2004): 6834
Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry
Authors: Seo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2004): 5488
Authors: Seo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2004): 5488
Evaluation of surface-enhanced resonance Raman scattering for quantitative DNA analysis
Authors: Faulds K, Smith WE, Graham D.
Journal: Anal Chem (2004): 412
Authors: Faulds K, Smith WE, Graham D.
Journal: Anal Chem (2004): 412
Blue light-induced generation of reactive oxygen species in photoreceptor ellipsoids requires mitochondrial electron transport
Authors: Yang JH, Basinger SF, Gross RL, Wu SM.
Journal: Invest Ophthalmol Vis Sci (2003): 1312
Authors: Yang JH, Basinger SF, Gross RL, Wu SM.
Journal: Invest Ophthalmol Vis Sci (2003): 1312
HLA-DRB fluorotyping by dark quenching and automated analysis
Authors: Slateva K, Elsner HA, Albis-Camps M, Blasczyk R.
Journal: Tissue Antigens (2001): 250
Authors: Slateva K, Elsner HA, Albis-Camps M, Blasczyk R.
Journal: Tissue Antigens (2001): 250
Influence of fluorophor dye labels on the migration behavior of polymerase chain reaction--amplified short tandem repeats during denaturing capillary electrophoresis
Authors: Hahn M, Wilhelm J, Pingoud A.
Journal: Electrophoresis (2001): 2691
Authors: Hahn M, Wilhelm J, Pingoud A.
Journal: Electrophoresis (2001): 2691
Design, synthesis, and spectroscopic properties of peptide-bridged fluorescence energy-transfer cassettes
Authors: Li Y, Glazer AN.
Journal: Bioconjug Chem (1999): 241
Authors: Li Y, Glazer AN.
Journal: Bioconjug Chem (1999): 241
Differential display with carboxy-X-rhodamine-labeled primers and the selection of differentially amplified cDNA fragments without cloning
Authors: Yoshikawa Y, Mukai H, Asada K, Hino F, Kato I.
Journal: Anal Biochem (1998): 82
Authors: Yoshikawa Y, Mukai H, Asada K, Hino F, Kato I.
Journal: Anal Biochem (1998): 82
Comparison of fluorescence energy transfer primers with different donor-acceptor dye combinations
Authors: Hung SC, Mathies RA, Glazer AN.
Journal: Anal Biochem (1998): 32
Authors: Hung SC, Mathies RA, Glazer AN.
Journal: Anal Biochem (1998): 32
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