5(6)-ROX, SE [5-(and-6)-Carboxy-X-rhodamine, succinimidyl ester] Mixed isomers

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Physical properties

Molecular weight	631.67
Solvent	DMSO

Spectral properties

Correction Factor (280 nm)	0.168
Extinction coefficient (cm ^-1 M ^-1)	82000
Excitation (nm)	578
Emission (nm)	604
Quantum yield	0.94¹

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

114616-32-9

Molecular weight

631.67

Correction Factor (280 nm)

0.168

Extinction coefficient (cm ^-1 M ^-1)

82000

Excitation (nm)

578

Emission (nm)

604

Quantum yield

0.94¹

5(6)-ROX, SE is the succinimidyl esters of 5(6)-ROX. It is the mixed isomers of ROX dye. The ratio of 6-isomer/5-isomer is in the range 3/1 to 6/1. This product is the primary labeling reagents for preparing various ROX-based bioconjugates. Compared to other rhodamines, ROX is very unstable. Cautions must be exercised to prevent the deterioration during storage.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5(6)-ROX, SE [5-(and-6)-Carboxy-X-rhodamine, succinimidyl ester] *Mixed isomers* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	158.311 µL	791.553 µL	1.583 mL	7.916 mL	15.831 mL
5 mM	31.662 µL	158.311 µL	316.621 µL	1.583 mL	3.166 mL
10 mM	15.831 µL	79.155 µL	158.311 µL	791.553 µL	1.583 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (280 nm)	0.168
Extinction coefficient (cm ^-1 M ^-1)	82000
Excitation (nm)	578
Emission (nm)	604
Quantum yield	0.94¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (260 nm)	Correction Factor (280 nm)
5(6)-CR6G, SE [5-(and 6)-Carboxyrhodamine 6G, succinimidyl ester] Mixed isomers	522	546	94000	0.24	0.214
5(6)-TAMRA, SE [5-(and-6)-Carboxytetramethylrhodamine, succinimidyl ester] CAS 246256-50-8	552	578	90000	0.32	0.178
5(6)-FAM, SE [5-(and-6)-Carboxyfluorescein, succinimidyl ester] CAS 117548-22-8	493	517	83000	0.32	0.178
6-ROX-AHA, SE	578	604	82000	-	0.168

Images

Figure 1. Chemical structure for 5(6)-ROX, SE [5-(and-6)-Carboxy-X-rhodamine, succinimidyl ester] *Mixed isomers*

References

View all 11 references: Citation Explorer

Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926

Adsorption of oligonucleotides on PMMA/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence
Authors: Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C.
Journal: Langmuir (2004): 6834

Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry
Authors: Seo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2004): 5488

Evaluation of surface-enhanced resonance Raman scattering for quantitative DNA analysis
Authors: Faulds K, Smith WE, Graham D.
Journal: Anal Chem (2004): 412

Blue light-induced generation of reactive oxygen species in photoreceptor ellipsoids requires mitochondrial electron transport
Authors: Yang JH, Basinger SF, Gross RL, Wu SM.
Journal: Invest Ophthalmol Vis Sci (2003): 1312

HLA-DRB fluorotyping by dark quenching and automated analysis
Authors: Slateva K, Elsner HA, Albis-Camps M, Blasczyk R.
Journal: Tissue Antigens (2001): 250

Influence of fluorophor dye labels on the migration behavior of polymerase chain reaction--amplified short tandem repeats during denaturing capillary electrophoresis
Authors: Hahn M, Wilhelm J, Pingoud A.
Journal: Electrophoresis (2001): 2691

Design, synthesis, and spectroscopic properties of peptide-bridged fluorescence energy-transfer cassettes
Authors: Li Y, Glazer AN.
Journal: Bioconjug Chem (1999): 241

Differential display with carboxy-X-rhodamine-labeled primers and the selection of differentially amplified cDNA fragments without cloning
Authors: Yoshikawa Y, Mukai H, Asada K, Hino F, Kato I.
Journal: Anal Biochem (1998): 82

Comparison of fluorescence energy transfer primers with different donor-acceptor dye combinations
Authors: Hung SC, Mathies RA, Glazer AN.
Journal: Anal Biochem (1998): 32