logo
AAT Bioquest

5(6)-TAMRA [5(6)-Carboxytetramethylrhodamine] *CAS 98181-63-6*

Product Image
Product Image
Gallery Image 1
Ordering information
Price
Catalog Number
Unit Size
Quantity
Add to cart
Additional ordering information
Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
Request quotation
Physical properties
Molecular weight430.45
SolventDMSO
Spectral properties
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.178
Extinction coefficient (cm -1 M -1)90000
Excitation (nm)552
Emission (nm)578
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Related products
Rhodamine 700 *CAS 63561-42-2*
Rhodamine 800 *CAS 137993-41-0*
Rhodamine B *CAS 81-88-9*
Rhodamine 6G *CAS 989-38-8*
Sulforhodamine 101, sodium salt
Sulforhodamine B *CAS 3520-42-1*
Sulforhodamine G *CAS 5873-16-5*
Rhodamine 101 Inner Salt *CAS 116450-56-7*
Rhodamine 101 Chloride Salt *CAS 64339-18-0*
Sulforhodamine 101 sodium salt *CAS 465544-24-5*
Rhodamine 110 *CAS 13558-31-1*
5-CR110 [5-Carboxyrhodamine 110] *Single isomer*
6-CR110 [6-Carboxyrhodamine 110] *Single isomer*
5-CR6G [5-Carboxyrhodamine 6G] *Single isomer*
6-CR6G [6-Carboxyrhodamine 6G] *Single isomer*
5(6)-CR6G, SE [5-(and 6)-Carboxyrhodamine 6G, succinimidyl ester] *Mixed isomers*
6-CR6G, SE [6-Carboxyrhodamine 6G, succinimidyl ester] *Single isomer*
5(6)-Carboxyrhodamine 6G cadaverine
5(6)-Carboxyrhodamine 6G ethylenediamine
5-CR6G, SE [5-Carboxyrhodamine 6G, succinimidyl ester] *Single isomer*
5-TAMRA [5-Carboxytetramethylrhodamine] *CAS 91809-66-4*
6-TAMRA [6-Carboxytetramethylrhodamine] *CAS 91809-67-5*
5(6)-TAMRA, SE [5-(and-6)-Carboxytetramethylrhodamine, succinimidyl ester] *CAS 246256-50-8*
5-ROX [5-Carboxy-X-rhodamine] *CAS 216699-35-3*
6-ROX [6-Carboxy-X-rhodamine] *CAS 194785-18-7*
5(6)-ROX, SE [5-(and-6)-Carboxy-X-rhodamine, succinimidyl ester] *Mixed isomers*
5-ROX, SE [5-Carboxy-X-rhodamine, succinimidyl ester] *CAS 209734-74-7*
6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester] *CAS#: 216699-36-4*
5(6)-TAMRA Maleimide [Tetramethylrhodamine-5-(and-6)-maleimide] *Mixed isomers*
5-TRITC [Tetramethylrhodamine-5-isothiocyanate] *CAS 80724-19-2*
6-TRITC [Tetramethylrhodamine-6-isothiocyanate] *CAS 80724-20-5*
Lissamine Rhodamine B Sulfonyl Chloride [Sulforhodamine B sulfonyl chloride] *CAS 62796-29-6*
Sulforhodamine 101 sulfonyl chloride [Texas Red®] *CAS 82354-19-6*
SRB-VAD-FMK [Sulforhodamine B-VAD-FMK]
Dihydrorhodamine 123 *CAS 109244-58-8*
Tetramethylrhodamine-dUTP *1 mM in Tris Buffer (pH 7.5)*
Rhodamine 123 *CAS 62669-70-9*
Rhodamine B, hexyl ester, perchlorate
TMRE [Tetramethylrhodamine ethyl ester] *CAS#: 115532-52-0*
TMRM [Tetramethylrhodamine methyl ester] *CAS#: 115532-50-8*
Phalloidin-Tetramethylrhodamine Conjugate
5-dR6G [5-Carboxy-4,7-dichlororhodamine 6G]
6-dR6G [6-Carboxy-4,7-dichlororhodamine 6G]
5-dR110 [5-Carboxy-4,7-dichlororhodamine 110]
6-dR110 [6-Carboxy-4,7-dichlororhodamine 110]
Bis-L-Aspartic acid Beta-rhodamine 110
Bis-Arg-rhodamine 110
Sulforhodamine 101 *CAS 60311-02-6*
5-Carboxyrhodamine 6G maleimide
Rhodamine aldehyde [5-TAMRA aldehyde]
Show More (40)

OverviewpdfSDSpdfProtocol


CAS
98181-63-6
Molecular weight
430.45
Correction Factor (260 nm)
0.32
Correction Factor (280 nm)
0.178
Extinction coefficient (cm -1 M -1)
90000
Excitation (nm)
552
Emission (nm)
578
TAMRA is one of the most popular fluorophores used in various bioconjugations. TMR is a bright orange fluorophore. 5(6)-TAMRA is the mixture of two carboxy tetramethylrhodamine (TMR) isomers. It is used to modify amino and hydroxy groups using EDC-mediated couplings. It can be readily convert to the amine-reactive 5(6)-TAMRA, SE.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5(6)-TAMRA [5(6)-Carboxytetramethylrhodamine] *CAS 98181-63-6* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM232.315 µL1.162 mL2.323 mL11.616 mL23.232 mL
5 mM46.463 µL232.315 µL464.63 µL2.323 mL4.646 mL
10 mM23.232 µL116.158 µL232.315 µL1.162 mL2.323 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.178
Extinction coefficient (cm -1 M -1)90000
Excitation (nm)552
Emission (nm)578

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
5(6)-CR6G [5-(and 6)-Carboxyrhodamine 6G]522546940000.240.214
5-TAMRA, SE [5-Carboxytetramethylrhodamine, succinimidyl ester] *CAS#: 150810-68-7*552578900000.320.178
6-TAMRA, SE [6-Carboxytetramethylrhodamine, succinimidyl ester] *CAS#: 150810-69-8*552578900000.320.178
5(6)-ROX [5-(and 6)-Carboxy-X-rhodamine] *CAS 198978-94-8*57860482000-0.168
5(6)-TRITC [Tetramethylrhodamine-5-(and-6)-isothiocyanate] *CAS 95197-95-8*5445701000000.270.34
6-TAMRA Maleimide [Tetramethylrhodamine-6-maleimide] *CAS 174568-68-4*552578900000.320.178
5-TAMRA Maleimide [Tetramethylrhodamine-5-maleimide] *CAS 154480-30-5*552578900000.320.178
5(6)-FAM [5-(and-6)-Carboxyfluorescein] *CAS 72088-94-9*493517830000.320.178
5-TAMRA cadaverine552578900000.320.178
6-TAMRA cadaverine552578900000.320.178
5-TAMRA ethylenediamine552578900000.320.178
6-TAMRA ethylenediamine552578900000.320.178
5-TAMRA azide552578900000.320.178
5-TAMRA alkyne552578900000.320.178
6-TAMRA azide552578900000.320.178
6-TAMRA alkyne552578900000.320.178
6-TAMRA CPG *1000 Å*552578900000.320.178
Show More (8)

Images


Citations


View all 8 citations: Citation Explorer
Adjusting Heterodimeric Coiled-Coils (K/E Zipper) to Connect Autophagy-Inducing Peptide with Cell-Penetrating Peptide
Authors: Hakata, Yoshiyuki and Yamashita, Kazuma and Hashimoto, Sonoko and Ohtsuki, Takashi and Miyazawa, Masaaki and Kitamatsu, Mizuki
Journal: Pharmaceutics (2023): 1048
Complementary leucine zippering system for effective intracellular delivery of proteins by cell-penetrating peptides
Authors: Kitamatsu, Mizuki and Yuasa, Hiroki and Ohtsuki, Takashi and Michiue, Hiroyuki
Journal: Bioorganic \& Medicinal Chemistry (2021): 116036
Intracellular delivery of a peptide nucleic acid-based hybrid of an autophagy inducing peptide with a cell-penetrating peptide
Authors: Hakata, Yoshiyuki and Ishikawa, Suzuka and Ohtsuki, Takashi and Miyazawa, Masaaki and Kitamatsu, Mizuki
Journal: Organic \& Biomolecular Chemistry (2020): 1978--1986
Amikacin liposome inhalation suspension (ALIS) penetrates non-tuberculous mycobacterial biofilms and enhances amikacin uptake into macrophages
Authors: Zhang, Jimin and Leifer, Franziska and Rose, Sasha and Chun, Dung Yu and Thaisz, Jill and Herr, Tracey and Nashed, Mary and Joseph, Jayanthi and Perkins, Walter R and DiPetrillo, Keith
Journal: Frontiers in microbiology (2018)
CCR2 signal facilitates thymic egress by priming thymocyte responses to sphingosine-1-phosphate
Authors: Aili, Abudureyimujiang and Zhang, Jie and Wu, Jia and Wu, Haoming and Sun, Xiuyuan and He, Qihua and Jin, Rong and Zhang, Yu
Journal: Frontiers in immunology (2018): 1263
Design and Characterization of Two Bifunctional Cryptophane A-Based Host Molecules for Xenon Magnetic Resonance Imaging Applications
Authors: Rossella, Federica and Rose, Honor May and Witte, Christopher and Jayapaul, Jabadurai and Schr&ouml;der, Leif
Journal: ChemPlusChem (2014): 1463--1471
Human erythrocytes as drug carriers: loading efficiency and side effects of hypotonic dialysis, chlorpromazine treatment and fusion with liposomes
Authors: Favretto, ME and Cluitmans, JCA and Bosman, GJCGM and Brock, R
Journal: Journal of Controlled Release (2013): 343--351
Advances in Quantitative FRET-Based Methods for Studying Nucleic Acids
Authors: Preus, S&oslash;ren and Wilhelmsson, L Marcus
Journal: ChemBioChem (2012): 1990--2001

References


View all 96 references: Citation Explorer
Optical detection of DNA hybridization based on fluorescence quenching of tagged oligonucleotide probes by gold nanoparticles
Authors: Wu ZS, Jiang JH, Fu L, Shen GL, Yu RQ.
Journal: Anal Biochem (2006): 22
Masking oligonucleotides improve sensitivity of mutation detection based on guanine quenching
Authors: Maruyama T, Shinohara T, Hosogi T, Ichinose H, Kamiya N, Goto M.
Journal: Anal Biochem (2006): 8
A fluorescence polarization assay for screening inhibitors against the ribonuclease H activity of HIV-1 reverse transcriptase
Authors: Nakayama GR, Bingham P, Tan D, Maegley KA.
Journal: Anal Biochem (2006): 260
High efficiency micellar electrokinetic chromatography of hydrophobic analytes on poly(dimethylsiloxane) microchips
Authors: Roman GT, McDaniel K, Culbertson CT.
Journal: Analyst (2006): 194
Metal-enhanced fluorescence-based RNA sensing
Authors: Aslan K, Huang J, Wilson GM, Geddes CD.
Journal: J Am Chem Soc (2006): 4206
Fluorescence anisotropy and FRET studies of G-quadruplex formation in presence of different cations
Authors: Juskowiak B, Galezowska E, Zawadzka A, Gluszynska A, Takenaka S.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2006): 835
Electrostatic-gated transport in chemically modified glass nanopore electrodes
Authors: Wang G, Zhang B, Wayment JR, Harris JM, White HS.
Journal: J Am Chem Soc (2006): 7679
A targeted protease substrate for a quantitative determination of protease activities in the endolysosomal pathway
Authors: Fischer R, Bachle D, Fotin-Mleczek M, Jung G, Kalbacher H, Brock R.
Journal: Chembiochem (2006): 1428
G Quadruplex-Based FRET Probes with the Thrombin-Binding Aptamer (TBA) Sequence Designed for the Efficient Fluorometric Detection of the Potassium Ion
Authors: Nagatoishi S, Nojima T, Galezowska E, Juskowiak B, Takenaka S.
Journal: Chembiochem (2006): 1730
Fluorescence properties of fluorescein, tetramethylrhodamine and Texas Red linked to a DNA aptamer
Authors: Unruh JR, Gokulrangan G, Wilson GS, Johnson CK.
Journal: Photochem Photobiol (2005): 682