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5(6)-TAMRA ethylenediamine

5(6)-TAMRA ethylenediamine is a building block for developing TAMRA bioconjugates. TAMRA is one of the most popular bright orange fluorophores used in various bioconjugations. 5(6)-TAMRA is the mixture of two carboxy tetramethylrhodamine (TMR) isomers.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5(6)-TAMRA ethylenediamine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM142.739 µL713.694 µL1.427 mL7.137 mL14.274 mL
5 mM28.548 µL142.739 µL285.478 µL1.427 mL2.855 mL
10 mM14.274 µL71.369 µL142.739 µL713.694 µL1.427 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
5(6)-FAM ethylenediamine493517830000.320.178
5(6)-TAMRA cadaverine552578900000.320.178
5(6)-TAMRA, SE [5-(and-6)-Carboxytetramethylrhodamine, succinimidyl ester] *CAS 246256-50-8*552578900000.320.178
5(6)-TAMRA Maleimide [Tetramethylrhodamine-5-(and-6)-maleimide] *Mixed isomers*552578900000.320.178

Citations

View all 4 citations: Citation Explorer
Quick-Responsive Polymer-Based Thermo-Sensitive Liposomes for Controlled Doxorubicin Release and Chemotherapy
Authors: Mo, Yulin and Du, Hongliang and Chen, Binlong and Liu, Dechun and Yin, Qingqing and Yan, Yue and Wang, Zenghui and Wan, Fangjie and Qi, Tong and Wang, Yaoqi and others, undefined
Journal: ACS Biomaterials Science & Engineering (2019)
Design and Characterization of Two Bifunctional Cryptophane A-Based Host Molecules for Xenon Magnetic Resonance Imaging Applications
Authors: Rossella, Federica and Rose, Honor May and Witte, Christopher and Jayapaul, Jabadurai and Schröder, Leif
Journal: ChemPlusChem (2014): 1463--1471
Human erythrocytes as drug carriers: loading efficiency and side effects of hypotonic dialysis, chlorpromazine treatment and fusion with liposomes
Authors: Favretto, ME and Cluitmans, JCA and Bosman, GJCGM and Brock, R
Journal: Journal of Controlled Release (2013): 343--351
Advances in Quantitative FRET-Based Methods for Studying Nucleic Acids
Authors: Preus, Søren and Wilhelmsson, L Marcus
Journal: ChemBioChem (2012): 1990--2001

References

View all 96 references: Citation Explorer
Metal-enhanced fluorescence-based RNA sensing
Authors: Aslan K, Huang J, Wilson GM, Geddes CD.
Journal: J Am Chem Soc (2006): 4206
G Quadruplex-Based FRET Probes with the Thrombin-Binding Aptamer (TBA) Sequence Designed for the Efficient Fluorometric Detection of the Potassium Ion
Authors: Nagatoishi S, Nojima T, Galezowska E, Juskowiak B, Takenaka S.
Journal: Chembiochem (2006): 1730
A targeted protease substrate for a quantitative determination of protease activities in the endolysosomal pathway
Authors: Fischer R, Bachle D, Fotin-Mleczek M, Jung G, Kalbacher H, Brock R.
Journal: Chembiochem (2006): 1428
Electrostatic-gated transport in chemically modified glass nanopore electrodes
Authors: Wang G, Zhang B, Wayment JR, Harris JM, White HS.
Journal: J Am Chem Soc (2006): 7679
Fluorescence anisotropy and FRET studies of G-quadruplex formation in presence of different cations
Authors: Juskowiak B, Galezowska E, Zawadzka A, Gluszynska A, Takenaka S.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2006): 835
Page updated on December 11, 2024

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Physical properties

Molecular weight

700.58

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.32

Correction Factor (280 nm)

0.178

Extinction coefficient (cm -1 M -1)

90000

Excitation (nm)

552

Emission (nm)

578

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501