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5(6)-TAMRA, SE [5-(and-6)-Carboxytetramethylrhodamine, succinimidyl ester] *CAS 246256-50-8*
The succinimidyl esters of 5-TAMRA, 6-TAMRA or the mixed isomers are the primary labeling reagents for the preparation of orange fluorescent bioconjugates, including peptide, protein, nucleotide and nucleic acid conjugates, especially fluorescent antibodies and avidin derivatives used in immunochemistry. TMR dyes have also been widely used as acceptors for FAM fluorophores in a variety of FRET studies.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 5(6)-TAMRA, SE [5-(and-6)-Carboxytetramethylrhodamine, succinimidyl ester] *CAS 246256-50-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 189.563 µL | 947.813 µL | 1.896 mL | 9.478 mL | 18.956 mL |
| 5 mM | 37.913 µL | 189.563 µL | 379.125 µL | 1.896 mL | 3.791 mL |
| 10 mM | 18.956 µL | 94.781 µL | 189.563 µL | 947.813 µL | 1.896 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
| 5(6)-CR6G, SE [5-(and 6)-Carboxyrhodamine 6G, succinimidyl ester] *Mixed isomers* | 522 | 546 | 94000 | 0.24 | 0.214 |
| 5(6)-ROX, SE [5-(and-6)-Carboxy-X-rhodamine, succinimidyl ester] *Mixed isomers* | 578 | 604 | 82000 | - | 0.168 |
| 5(6)-TAMRA Maleimide [Tetramethylrhodamine-5-(and-6)-maleimide] *Mixed isomers* | 552 | 578 | 90000 | 0.32 | 0.178 |
| 5(6)-FAM, SE [5-(and-6)-Carboxyfluorescein, succinimidyl ester] *CAS 117548-22-8* | 493 | 517 | 83000 | 0.32 | 0.178 |
| 5(6)-TAMRA ethylenediamine | 552 | 578 | 90000 | 0.32 | 0.178 |
| 5(6)-TAMRA cadaverine | 552 | 578 | 90000 | 0.32 | 0.178 |
Citations
View all 9 citations: Citation Explorer
Chemistry-driven translocation of glycosylated proteins in mice
Authors: Yamada, Kenshiro and Mukaimine, Akari and Nakamura, Akiko and Kusakari, Yuriko and Pradipta, Ambara R and Chang, Tsung-Che and Tanaka, Katsunori
Journal: Nature Communications (2024): 7409
Authors: Yamada, Kenshiro and Mukaimine, Akari and Nakamura, Akiko and Kusakari, Yuriko and Pradipta, Ambara R and Chang, Tsung-Che and Tanaka, Katsunori
Journal: Nature Communications (2024): 7409
Biofunctionalized dissolvable hydrogel microbeads enable efficient characterization of native protein complexes
Authors: Shao, Xinyang and Tian, Meng and Yin, Junlong and Duan, Haifeng and Tian, Ye and Wang, Hui and Xia, Changsheng and Wang, Ziwei and Zhu, Yanxi and Wang, Yifan and others,
Journal: Nature Communications (2024): 8633
Authors: Shao, Xinyang and Tian, Meng and Yin, Junlong and Duan, Haifeng and Tian, Ye and Wang, Hui and Xia, Changsheng and Wang, Ziwei and Zhu, Yanxi and Wang, Yifan and others,
Journal: Nature Communications (2024): 8633
Three-dimensional printing of click functionalized, peptide patterned scaffolds for osteochondral tissue engineering
Authors: Guo, Jason L and Diaz-Gomez, Luis and Xie, Virginia Y and Bittner, Sean M and Jiang, Emily Y and Wang, Bonnie and Mikos, Antonios G
Journal: Bioprinting (2021): e00136
Authors: Guo, Jason L and Diaz-Gomez, Luis and Xie, Virginia Y and Bittner, Sean M and Jiang, Emily Y and Wang, Bonnie and Mikos, Antonios G
Journal: Bioprinting (2021): e00136
Cancer Discrimination By On-cell N-glycan Ligation
Authors: Shogo, Nomura and Yasuko, Egawa and Sayaka, Urano and Tsuyoshi, Tahara and Yasuyoshi, Watanabe and Katsunori, Tanaka
Journal: Communications Chemistry (2020)
Authors: Shogo, Nomura and Yasuko, Egawa and Sayaka, Urano and Tsuyoshi, Tahara and Yasuyoshi, Watanabe and Katsunori, Tanaka
Journal: Communications Chemistry (2020)
Cancer discrimination by on-cell N-glycan ligation
Authors: Nomura, Shogo and Egawa, Yasuko and Urano, Sayaka and Tahara, Tsuyoshi and Watanabe, Yasuyoshi and Tanaka, Katsunori
Journal: Communications Chemistry (2020): 1--7
Authors: Nomura, Shogo and Egawa, Yasuko and Urano, Sayaka and Tahara, Tsuyoshi and Watanabe, Yasuyoshi and Tanaka, Katsunori
Journal: Communications Chemistry (2020): 1--7
References
View all 96 references: Citation Explorer
Masking oligonucleotides improve sensitivity of mutation detection based on guanine quenching
Authors: Maruyama T, Shinohara T, Hosogi T, Ichinose H, Kamiya N, Goto M.
Journal: Anal Biochem (2006): 8
Authors: Maruyama T, Shinohara T, Hosogi T, Ichinose H, Kamiya N, Goto M.
Journal: Anal Biochem (2006): 8
G Quadruplex-Based FRET Probes with the Thrombin-Binding Aptamer (TBA) Sequence Designed for the Efficient Fluorometric Detection of the Potassium Ion
Authors: Nagatoishi S, Nojima T, Galezowska E, Juskowiak B, Takenaka S.
Journal: Chembiochem (2006): 1730
Authors: Nagatoishi S, Nojima T, Galezowska E, Juskowiak B, Takenaka S.
Journal: Chembiochem (2006): 1730
A targeted protease substrate for a quantitative determination of protease activities in the endolysosomal pathway
Authors: Fischer R, Bachle D, Fotin-Mleczek M, Jung G, Kalbacher H, Brock R.
Journal: Chembiochem (2006): 1428
Authors: Fischer R, Bachle D, Fotin-Mleczek M, Jung G, Kalbacher H, Brock R.
Journal: Chembiochem (2006): 1428
Electrostatic-gated transport in chemically modified glass nanopore electrodes
Authors: Wang G, Zhang B, Wayment JR, Harris JM, White HS.
Journal: J Am Chem Soc (2006): 7679
Authors: Wang G, Zhang B, Wayment JR, Harris JM, White HS.
Journal: J Am Chem Soc (2006): 7679
Fluorescence anisotropy and FRET studies of G-quadruplex formation in presence of different cations
Authors: Juskowiak B, Galezowska E, Zawadzka A, Gluszynska A, Takenaka S.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2006): 835
Authors: Juskowiak B, Galezowska E, Zawadzka A, Gluszynska A, Takenaka S.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2006): 835
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