5(6)-TAMRA, SE [5-(and-6)-Carboxytetramethylrhodamine, succinimidyl ester] CAS 246256-50-8

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Physical properties

Molecular weight	527.53
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	90000
Excitation (nm)	552
Emission (nm)	578

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

246256-50-8

Molecular weight

527.53

Correction Factor (260 nm)

0.32

Correction Factor (280 nm)

0.178

Extinction coefficient (cm ^-1 M ^-1)

90000

Excitation (nm)

552

Emission (nm)

578

The succinimidyl esters of 5-TAMRA, 6-TAMRA or the mixed isomers are the primary labeling reagents for the preparation of orange fluorescent bioconjugates, including peptide, protein, nucleotide and nucleic acid conjugates, especially fluorescent antibodies and avidin derivatives used in immunochemistry. TMR dyes have also been widely used as acceptors for FAM fluorophores in a variety of FRET studies.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5(6)-TAMRA, SE [5-(and-6)-Carboxytetramethylrhodamine, succinimidyl ester] *CAS 246256-50-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	189.563 µL	947.813 µL	1.896 mL	9.478 mL	18.956 mL
5 mM	37.913 µL	189.563 µL	379.125 µL	1.896 mL	3.791 mL
10 mM	18.956 µL	94.781 µL	189.563 µL	947.813 µL	1.896 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	90000
Excitation (nm)	552
Emission (nm)	578

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (260 nm)	Correction Factor (280 nm)
5(6)-CR6G, SE [5-(and 6)-Carboxyrhodamine 6G, succinimidyl ester] Mixed isomers	522	546	94000	0.24	0.214
5(6)-ROX, SE [5-(and-6)-Carboxy-X-rhodamine, succinimidyl ester] Mixed isomers	578	604	82000	-	0.168
5(6)-TAMRA Maleimide [Tetramethylrhodamine-5-(and-6)-maleimide] Mixed isomers	552	578	90000	0.32	0.178
5(6)-FAM, SE [5-(and-6)-Carboxyfluorescein, succinimidyl ester] CAS 117548-22-8	493	517	83000	0.32	0.178
5(6)-TAMRA ethylenediamine	552	578	90000	0.32	0.178
5(6)-TAMRA cadaverine	552	578	90000	0.32	0.178

Images

Figure 1. Chemical structure for 5(6)-TAMRA, SE [5-(and-6)-Carboxytetramethylrhodamine, succinimidyl ester] *CAS 246256-50-8*

Citations

View all 7 citations: Citation Explorer

Three-dimensional printing of click functionalized, peptide patterned scaffolds for osteochondral tissue engineering
Authors: Guo, Jason L and Diaz-Gomez, Luis and Xie, Virginia Y and Bittner, Sean M and Jiang, Emily Y and Wang, Bonnie and Mikos, Antonios G
Journal: Bioprinting (2021): e00136

Cancer Discrimination By On-cell N-glycan Ligation
Authors: Shogo, Nomura and Yasuko, Egawa and Sayaka, Urano and Tsuyoshi, Tahara and Yasuyoshi, Watanabe and Katsunori, Tanaka
Journal: Communications Chemistry (2020)

Cancer discrimination by on-cell N-glycan ligation
Authors: Nomura, Shogo and Egawa, Yasuko and Urano, Sayaka and Tahara, Tsuyoshi and Watanabe, Yasuyoshi and Tanaka, Katsunori
Journal: Communications Chemistry (2020): 1--7

Amikacin liposome inhalation suspension (ALIS) penetrates non-tuberculous mycobacterial biofilms and enhances amikacin uptake into macrophages
Authors: Zhang, Jimin and Leifer, Franziska and Rose, Sasha and Chun, Dung Yu and Thaisz, Jill and Herr, Tracey and Nashed, Mary and Joseph, Jayanthi and Perkins, Walter R and DiPetrillo, Keith
Journal: Frontiers in microbiology (2018)

Design and Characterization of Two Bifunctional Cryptophane A-Based Host Molecules for Xenon Magnetic Resonance Imaging Applications
Authors: Rossella, Federica and Rose, Honor May and Witte, Christopher and Jayapaul, Jabadurai and Schröder, Leif
Journal: ChemPlusChem (2014): 1463--1471

Human erythrocytes as drug carriers: loading efficiency and side effects of hypotonic dialysis, chlorpromazine treatment and fusion with liposomes
Authors: Favretto, ME and Cluitmans, JCA and Bosman, GJCGM and Brock, R
Journal: Journal of Controlled Release (2013): 343--351

Advances in Quantitative FRET-Based Methods for Studying Nucleic Acids
Authors: Preus, Søren and Wilhelmsson, L Marcus
Journal: ChemBioChem (2012): 1990--2001

References

View all 96 references: Citation Explorer

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Masking oligonucleotides improve sensitivity of mutation detection based on guanine quenching
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Journal: Anal Biochem (2006): 8

A fluorescence polarization assay for screening inhibitors against the ribonuclease H activity of HIV-1 reverse transcriptase
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High efficiency micellar electrokinetic chromatography of hydrophobic analytes on poly(dimethylsiloxane) microchips
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Metal-enhanced fluorescence-based RNA sensing
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Fluorescence anisotropy and FRET studies of G-quadruplex formation in presence of different cations
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Journal: Spectrochim Acta A Mol Biomol Spectrosc (2006): 835

Electrostatic-gated transport in chemically modified glass nanopore electrodes
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Journal: J Am Chem Soc (2006): 7679

A targeted protease substrate for a quantitative determination of protease activities in the endolysosomal pathway
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Journal: Chembiochem (2006): 1428

G Quadruplex-Based FRET Probes with the Thrombin-Binding Aptamer (TBA) Sequence Designed for the Efficient Fluorometric Detection of the Potassium Ion
Authors: Nagatoishi S, Nojima T, Galezowska E, Juskowiak B, Takenaka S.
Journal: Chembiochem (2006): 1730

Fluorescence properties of fluorescein, tetramethylrhodamine and Texas Red linked to a DNA aptamer
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Journal: Photochem Photobiol (2005): 682