Name: 5(6)-TAMRA, SE [5-(and-6)-Carboxytetramethylrhodamine, succinimidyl ester] *CAS 246256-50-8*
Brand: AAT Bioquest
SKU: 370
Availability: InStock

5(6)-TAMRA, SE

5-(and-6)-Carboxytetramethylrhodamine, succinimidyl ester; CAS 246256-50-8

The succinimidyl esters of 5-TAMRA, 6-TAMRA or the mixed isomers are the primary labeling reagents for the preparation of orange fluorescent bioconjugates, including peptide, protein, nucleotide and nucleic acid conjugates, especially fluorescent antibodies and avidin derivatives used in immunochemistry. TMR dyes have also been widely used as acceptors for FAM fluorophores in a variety of FRET studies.

Protocol

COO

COA

Catalog	Size	Price
370	25 mg	Price
371	100 mg	Price
372	1 g	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5(6)-TAMRA, SE [5-(and-6)-Carboxytetramethylrhodamine, succinimidyl ester] *CAS 246256-50-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	189.563 µL	947.813 µL	1.896 mL	9.478 mL	18.956 mL
5 mM	37.913 µL	189.563 µL	379.125 µL	1.896 mL	3.791 mL
10 mM	18.956 µL	94.781 µL	189.563 µL	947.813 µL	1.896 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	527.53 g/mol	=	mL	x	mM	=	mmol

Citations

View all 9 citations: Citation Explorer

Chemistry-driven translocation of glycosylated proteins in mice

Authors:

Yamada, Kenshiro and Mukaimine, Akari and Nakamura, Akiko and Kusakari, Yuriko and Pradipta, Ambara R and Chang, Tsung-Che and Tanaka, Katsunori

Journal:

Nature Communications (2024): 7409

Biofunctionalized dissolvable hydrogel microbeads enable efficient characterization of native protein complexes

Authors:

Shao, Xinyang and Tian, Meng and Yin, Junlong and Duan, Haifeng and Tian, Ye and Wang, Hui and Xia, Changsheng and Wang, Ziwei and Zhu, Yanxi and Wang, Yifan and others,

Journal:

Nature Communications (2024): 8633

Three-dimensional printing of click functionalized, peptide patterned scaffolds for osteochondral tissue engineering

Authors:

Guo, Jason L and Diaz-Gomez, Luis and Xie, Virginia Y and Bittner, Sean M and Jiang, Emily Y and Wang, Bonnie and Mikos, Antonios G

Journal:

Bioprinting (2021): e00136

Cancer Discrimination By On-cell N-glycan Ligation

Authors:

Shogo, Nomura and Yasuko, Egawa and Sayaka, Urano and Tsuyoshi, Tahara and Yasuyoshi, Watanabe and Katsunori, Tanaka

Journal:

Communications Chemistry (2020)

Cancer discrimination by on-cell N-glycan ligation

Authors:

Nomura, Shogo and Egawa, Yasuko and Urano, Sayaka and Tahara, Tsuyoshi and Watanabe, Yasuyoshi and Tanaka, Katsunori

Journal:

Communications Chemistry (2020): 1--7

References

View all 96 references: Citation Explorer

Masking oligonucleotides improve sensitivity of mutation detection based on guanine quenching

Authors:

Maruyama T, Shinohara T, Hosogi T, Ichinose H, Kamiya N, Goto M.

Journal:

Anal Biochem (2006): 8

G Quadruplex-Based FRET Probes with the Thrombin-Binding Aptamer (TBA) Sequence Designed for the Efficient Fluorometric Detection of the Potassium Ion

Authors:

Nagatoishi S, Nojima T, Galezowska E, Juskowiak B, Takenaka S.

Journal:

Chembiochem (2006): 1730

A targeted protease substrate for a quantitative determination of protease activities in the endolysosomal pathway

Authors:

Fischer R, Bachle D, Fotin-Mleczek M, Jung G, Kalbacher H, Brock R.

Journal:

Chembiochem (2006): 1428

Electrostatic-gated transport in chemically modified glass nanopore electrodes

Authors:

Wang G, Zhang B, Wayment JR, Harris JM, White HS.

Journal:

J Am Chem Soc (2006): 7679

Fluorescence anisotropy and FRET studies of G-quadruplex formation in presence of different cations

Authors:

Juskowiak B, Galezowska E, Zawadzka A, Gluszynska A, Takenaka S.

Journal:

Spectrochim Acta A Mol Biomol Spectrosc (2006): 835

Physical properties

Molecular weight	527.53
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	90000
Excitation (nm)	552
Emission (nm)	578

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	246256-50-8

Documents

Protocol
Safety Data Sheet (Catalog 370)
Safety Data Sheet (Catalog 371)
Safety Data Sheet (Catalog 372)
Certificate of Origin
Certificate of Analysis

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Page updated on October 24, 2025