5(6)-TRITC [Tetramethylrhodamine-5-(and-6)-isothiocyanate] CAS 95197-95-8

Ordering information

Price
Catalog Number
Unit Size
Quantity

Add to cart

Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Request quotation

Physical properties

Molecular weight	479.98
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.27
Correction Factor (280 nm)	0.34
Extinction coefficient (cm ^-1 M ^-1)	100000
Excitation (nm)	544
Emission (nm)	570

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

95197-95-8

Molecular weight

479.98

Correction Factor (260 nm)

0.27

Correction Factor (280 nm)

0.34

Extinction coefficient (cm ^-1 M ^-1)

100000

Excitation (nm)

544

Emission (nm)

570

5(6)-TRITC is an amino-reactive labeling reagent that is widely used in preparing bioconjugates of proteins and nucleic acids. The resultant conjugates have similar spectral properties to those prepared from 5(6)-TAMRA, SE. However, the latter conjugates are much more stable. Cautions must be exercised for the storage of TRITC conjugates.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5(6)-TRITC [Tetramethylrhodamine-5-(and-6)-isothiocyanate] *CAS 95197-95-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	208.342 µL	1.042 mL	2.083 mL	10.417 mL	20.834 mL
5 mM	41.668 µL	208.342 µL	416.684 µL	2.083 mL	4.167 mL
10 mM	20.834 µL	104.171 µL	208.342 µL	1.042 mL	2.083 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (260 nm)	0.27
Correction Factor (280 nm)	0.34
Extinction coefficient (cm ^-1 M ^-1)	100000
Excitation (nm)	544
Emission (nm)	570

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (260 nm)	Correction Factor (280 nm)
5(6)-CR6G [5-(and 6)-Carboxyrhodamine 6G]	522	546	94000	0.24	0.214
5(6)-TAMRA [5(6)-Carboxytetramethylrhodamine] CAS 98181-63-6	552	578	90000	0.32	0.178
5(6)-ROX [5-(and 6)-Carboxy-X-rhodamine] CAS 198978-94-8	578	604	82000	-	0.168
5(6)-FAM [5-(and-6)-Carboxyfluorescein] CAS 72088-94-9	493	517	83000	0.32	0.178

Images

Figure 1. Chemical structure for 5(6)-TRITC [Tetramethylrhodamine-5-(and-6)-isothiocyanate] *CAS 95197-95-8*

References

View all 45 references: Citation Explorer

Recombinant human erythropoietin prevents ischemia-induced apoptosis and necrosis in explant cultures of the rat organ of Corti
Authors: Andreeva N, Nyamaa A, Haupt H, Gross J, Mazurek B.
Journal: Neurosci Lett (2006): 86

Mechanism of advanced glycation end products-induced hyperpermeability in endothelial cells.
Authors: Guo XH, Huang QB, Chen B, Wang SY, Hou FF, Fu N.
Journal: Sheng Li Xue Bao (2005): 205

Musculature of an illoricate predatory rotifer Asplanchnopus multiceps as revealed by phalloidin fluorescence and confocal microscopy
Authors: Kotikova EA, Raikova OI, Reuter M, Gustafsson MK.
Journal: Tissue Cell (2004): 189

Resonance energy transfer nanobiosensors based on affinity binding between apo-enzyme and its substrate
Authors: Chinnayelka S, McShane MJ.
Journal: Biomacromolecules (2004): 1657

Nontypeable Haemophilus influenzae strain 2019 produces a biofilm containing N-acetylneuraminic acid that may mimic sialylated O-linked glycans
Authors: Greiner LL, Watanabe H, Phillips NJ, Shao J, Morgan A, Zaleski A, Gibson BW, Apicella MA.
Journal: Infect Immun (2004): 4249

Fluorescent dyes modify properties of proteins used in microvascular research
Authors: Bingaman S, Huxley VH, Rumbaut RE.
Journal: Microcirculation (2003): 221

Maturity of pericytes in cerebral neocapillaries induced by growth factors: fluorescence immuno-histochemical analysis using confocal laser microscopy
Authors: Jariyapongskul A, Nakano A, Yamaguchi S, Nageswari K, Niimi H.
Journal: Clin Hemorheol Microcirc (2003): 417

The critical role of lymph nodes in corneal alloimmunization and graft rejection
Authors: Yamagami S, Dana MR.
Journal: Invest Ophthalmol Vis Sci (2001): 1293

C-terminal actin-binding sites of smooth muscle caldesmon switch actin between conformational states
Authors: Borovikov YS, Avrova SV, Vikhoreva NN, Vikhorev PG, Ermakov VS, Copel and O, Marston SB.
Journal: Int J Biochem Cell Biol (2001): 1151

Assessment of lectin-binding analysis for in situ detection of glycoconjugates in biofilm systems
Authors: Neu T, Swerhone GD, Lawrence JR.
Journal: Microbiology (2001): 299